Sulfogalactosylglycerolipid is involved in human gamete interaction

Weerachatyanukul, Wattana; Rattanachaiyanont, Manee; Carmona, Eurı́dice; Furimsky, Anna; Mai, Andrew; Shoushtarian, Ali; Sirichotiyakul, Supatra; Ballakier, Hanna; Leader, Arthur; Tanphaichitr, Nongnuj

doi:10.1002/mrd.1122

Cited by 44 publications

(39 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). Previous work on sperm ARSA has led to the proposal that it initiates sperm-ZP engagement by virtue of its ability to act as a receptor for sulfated sugar ligands that decorate the ZP glycoproteins (Tanphaichitr et al, 1993;White et al, 2000;Weerachatyanukul et al, 2001;Tantibhedhyangkul et al, 2002;Carmona et al, 2002a). This notion is supported by the demonstration that ARSA has the ability to bind to polysulfated glycans (Huang et al, 1984;O'Rand et al, 1988;Jones and Williams, 1990;Jones et al, 1996) and that the components of the ZP are highly sulfated in nature (Prasad et al, 2000).…”

Section: Discussionmentioning

confidence: 55%

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition

Redgrove

Anderson²,

McLaughlin³

et al. 2012

Molecular Human Reproduction

View full text Add to dashboard Cite

A unique characteristic of mammalian spermatozoa is that, upon ejaculation, they are unable to recognize and bind to an ovulated oocyte. These functional attributes are only realized following the cells' ascent of the female reproductive tract whereupon they undergo a myriad of biochemical and biophysical changes collectively referred to as 'capacitation'. We have previously shown that this functional transformation is, in part, engineered by the modification of the sperm surface architecture leading to the assembly and/or presentation of multimeric sperm -oocyte receptor complexes. In this study, we have extended our findings through the characterization of one such complex containing arylsulfatase A (ARSA), sperm adhesion molecule 1 (SPAM1) and the molecular chaperone, heat shock 70kDa protein 2 (HSPA2). Through the application of flow cytometry we revealed that this complex undergoes a capacitation-associated translocation to facilitate the repositioning of ARSA to the apical region of the human sperm head, a location compatible with a role in the mediation of sperm -zona pellucida (ZP) interactions. Conversely, SPAM1 appears to reorient away from the sperm surface, possibly reflecting its primary role in cumulus matrix dispersal preceding sperm -ZP recognition. The dramatic relocation of the complex was completely abolished by incubation of capacitating spermatozoa in exogenous cholesterol or broad spectrum protein kinase A (PKA) and tyrosine kinase inhibitors suggesting that it may be driven by alterations in membrane fluidity characteristics and concurrently by the activation of a capacitation-associated signal transduction pathway. Collectively these data afford novel insights into the sub-cellular localization and potential functions of multimeric protein complexes in human spermatozoa.

show abstract

Section: Discussionmentioning

confidence: 55%

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition

Redgrove

Anderson²,

McLaughlin³

et al. 2012

Molecular Human Reproduction

View full text Add to dashboard Cite

show abstract

“…used ( 13,14 ). Azure A is a cationic dye that reacts with anionic lipids, including sulfolipids ( 15,16 ).…”

Section: Methodsmentioning

confidence: 99%

“…Azure A is also useful for staining sulfolipids on thin layer chromatography (TLC) plates. The limit of detection (LOD) of the colorimetric assay for sulfolipids is around 10 µg in the original method and, despite modifi cations and improvements, is still around 0.3 µg ( 13,17 ). The LOD of Azure A staining of chromatographed lipids on high-performance (HP)TLC plates is even higher: at least 1 µg of a sulfolipid is required for detection.…”

Section: Methodsmentioning

confidence: 99%

Quantification of seminolipid by LC-ESI-MS/MS-multiple reaction monitoring: compensatory levels in Cgt mice

Kongmanas

Yaghoubian

et al. 2010

Journal of Lipid Research

Self Cite

View full text Add to dashboard Cite

This article is available online at http://www.jlr.org Seminolipid, 1-O -alkyl-2-O -acyl[ ␤ -D-(3 ′ -sulfatoxy)galactopyranosyl(1'-3)] sn -glycerol, also known as sulfogalactosylglycerolipid (SGG) and SM4g ( Fig. 1A ), is present selectively and substantially in mammalian male germ cells, comprising about 10 mol% of total lipids ( 1 ). SGG consists of glycerol with a sn -1 alkyl and sn -2 acyl chain as the lipid backbone, and a (3 ′ -sulfo)-galactopyranose ␤ -linked to the sn -3 position. Consistently across mammalian species studied so far, C16:0/C16:0 SGG is the main molecular species ( 1, 2 ), although minor molecular species, such as C16:0/C14:0, C14:0/C16:0, C15:0/C16:0, C17:0/C16:0, C16:0/C18:0, C18:0/C16:0, and C17:0/C18:0 SGG, representing < 10% of the main species, have also been described ( 3, 4 ). Accumulated evidence has pointed to the signifi cance of SGG for mammalian male reproduction ( 1 ). SGG on the sperm surface is involved in sperm-zona pellucida (ZP) binding and sperm-egg plasma membrane binding ( 5, 6 ). As a structurally ordered lipid, SGG participates in the formation of lipid rafts in the sperm head, which act as platforms for ZP binding ( 7-10 ). Genetic deletion of Cgt and Cst , coding for two enzymes (ceramide galactosyltransferase and cerebroside sulfotransferase, respectively) in the SGG biosynthetic pathway, leads to spermatogenesis arrest in the primary spermatocyte stage and, thus, male infertility ( 11, 12 ).Due to its physiological signifi cance, an accurate, sensitive, specifi c method for SGG quantifi cation is needed. A simple colorimetric Azure A assay has been conventionally

show abstract

“…However, a limited number of studies indicate a role for carbohydrate moieties of the sperm glycocalyx in early gamete interactions. Tanphaichitr et al reported that Fab antibody fragments against sulfogalactosylglycerolipid (SGG), the major sulfoglycolipid in mammalian spermatozoa, inhibited human and mouse sperm binding and that SGG-bearing liposomes bound to the human and mouse zona pellucida [70,71]. Mahony et al [72] demonstrated that mAbs against carbohydrate epitopes of CD52, a glycosylphosphatidyl inositol (GPI)-anchored sperm-surface glycoconjugate, inhibit human sperm-zona binding.…”

Section: The Sperm Glycocalyxmentioning

confidence: 99%

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Ab¹

2003

CMLS, Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Glycoconjugates in the mammalian reproductive tract are critical components of the molecular mechanisms that control sperm maturation, sperm transport and gamete interactions. In the oviduct of many species, sperm transport and maturation are regulated by protein-carbohydrate interactions that form a sperm reservoir. Subsequently, gamete interactions are mediated by the binding of lectin-like sperm proteins with carbohydrate moieties on the zona pellucida. The sperm glycocalyx is extensively modified during sperm transport and maturation. Multiple functions have been proposed for this dense carbohydrate layer overlying the sperm plasmalemma, and sperm-surface carbohydrates have been implicated in immune-mediated human infertility. The structure and function of glycoconjugates in the oviductal sperm reservoir, the zona pellucida, and on the sperm surface are reviewed.

show abstract

Sulfogalactosylglycerolipid is involved in human gamete interaction

Cited by 44 publications

References 77 publications

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition

Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition

Quantification of seminolipid by LC-ESI-MS/MS-multiple reaction monitoring: compensatory levels in Cgt mice

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Contact Info

Product

Resources

About