Summary of the First International Workshop on Human Primed LD Typing

Hartzman, Robert J.

doi:10.1111/j.1399-0039.1979.tb00785.x

Cited by 6 publications

References 19 publications

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The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

et al. 1981

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Using a number of intrafamilial PLTs raised against identical HLA haplotypes it has been possible to construct a model in an informative family defining the HLA-D region as a genetic system. This system consists of at least two regions separated by a recombination between HLA-D and GLO. In relation to the site of recombination, a minimum of one centromeric and three telomeric components can be identified per haplotype. - Fourteen PLTs raised and defined within the family were subsequently tested in a Caucasian population (n = 84) and in 13 unrelated, complete families. - It is concluded that the hypothetical model proposed for the HLA-D regions as a genetic system of linked loci, coding at the cell surface for associated but distinct components (at least four per haplotype), allows for typing of the components of the HLA-D system of any given haplotype. Serological typing of HLA-D components should, in the near future, provide a more convenient way of establishing component phenotypes than the present use of primed lymphocyte typing reagents. Among the components isolated, some have a high association with the classic alleles defined either by homozygous typing cells or DR serology. Others form the basis of cross-reactivity but their presence does not interfere with standard typing. Others, however, seem by their mere presence to be responsible for false assignments. - The concept of HLA-D as a genetic system clarifies many of the inconsistencies observed with a one-locus system.

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The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

et al. 1981

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Restimulation properties of human alloreactive cloned t-cell lines. Dissection ofHLA-D-Region alleles in population studies and in family segregation analysis

Pawelec

Wernet

1980

Immunogenetics

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Alloactivated human lymphocytes were cloned by limiting dilution. After 1 month in culture with T-cell growth factor several clones incorporated tritiated thymidine when stimulated with the appropriate allogeneic cells. Specificity of restimulation of two primed lymphocyte clones, designated 12-2 and 12-8, was studied in detail after varying periods of culture (up to 50 days). Clone 12-2 cells were stimulated only by cells expressing the HLA-Dw antigens of the original priming cells (Dw3); furthermore, this primed lymphocyte reagent specifically recognized antigens associated with only one of the three distinct Dw3-bearing haplotypes from an informative family (KOH). Clone 12-8 cells, on the other hand, failed to recognize Dw3 antigens in the random panel or on homozygous typing cells (including the original priming cell), but were strongly restimulated by certain cells expressing Dw4 antigens. In addition, within family KOH, these restimulating products segregated with another one of the three Dw3-bearing haplotypes but with none of the three Dw4-bearing haplotypes. These two clones exemplify a hitherto unknown precision in cellular typing of the HLA-D region. Clone 12-2 allows the discrimination of a probably rare and as yet undetected HLA-Dw3 subtypic specificity. Clone 12-8, on the other hand, apparently identifies an allelic system segregating with HLA but distinct from the HLA-D determinants definable by HTC-typing.

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HLA-linked B cell alloantigens of a new segregant series: Population and family studies of the SB antigens

et al. 1980

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Summary of the First International Workshop on Human Primed LD Typing

Cited by 6 publications

References 19 publications

The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

The HLA-D system: At least two loci and four distinct phenotypic traits per haplotype. Introduction to component typing in families and population by primed lymphocyte typing

Restimulation properties of human alloreactive cloned t-cell lines. Dissection ofHLA-D-Region alleles in population studies and in family segregation analysis

HLA-linked B cell alloantigens of a new segregant series: Population and family studies of the SB antigens

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