SUMO modification of LBD30 by SIZ1 regulates secondary cell wall formation in Arabidopsis thaliana

Liu, Chang; Yu, Hasi; Li, Laigeng

doi:10.1371/journal.pgen.1007928

Cited by 42 publications

(24 citation statements)

References 74 publications

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“…Previously reported LBD genes, including LBD29, function as transcriptional activators in different developmental processes (Soyano et al, 2008;Feng et al, 2012;Porco et al, 2016;Liu et al, 2019). In this study, the transgenic ProLBD29:LBD29 plants showed strong overexpression of the LBD29 gene (Fig.…”

Section: Discussionsupporting

confidence: 55%

LBD29-Involved Auxin Signaling Represses NAC Master Regulators and Fiber Wall Biosynthesis

Lee

Zhuo

et al. 2019

Plant Physiol.

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NAM, ATAF1/2 and CUC2 (NAC) domain transcription factors function as master switches in regulating secondary cell wall (SCW) biosynthesis in Arabidopsis (Arabidopsis thaliana) stems. Despite the importance of these NACs in fiber development, the upstream signal is still elusive. Using a large-scale mutant screening, we identified a dominant activation-tagging mutant, fiberless-d (fls-d), showing defective SCW development in stem fibers, similar to that of the nac secondary wall thickening promoting factor1-1 (nst1-1)nst3-3 double mutant. Overexpression of LATERAL ORGAN BOUNDARIES DOMAIN29 (LBD29) is responsible for the fls-d mutant phenotypes. By contrast, loss-of-function of LBD29, either in the dominant repression transgenic lines or in the transfer-DNA (T-DNA) insertion mutant lbd29-1, enhanced SCW development in fibers. Genetic analysis and transgenic studies demonstrated LBD29 depends on master regulators in mediating SCW biosynthesis, specifically NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1), NST2, and NST3. Increasing indole-3-acetic acid (IAA) levels, either in stem tissues above a N-1-naphthylphthalamic acid-treated region or in plants directly sprayed with IAA, inhibits fiber wall thickening. The inhibition effect of naphthylphthalamic acid treatment and exogenous IAA application depends on a known auxin signaling pathway involving AUXIN RESPONSE FACTOR7 (ARF7)/ARF19 and LBD29. These results demonstrate auxin is upstream of LBD29 in repressing NAC master regulators, and therefore shed new light on the regulation of SCW biosynthesis in Arabidopsis.

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Section: Discussionsupporting

confidence: 55%

LBD29-Involved Auxin Signaling Represses NAC Master Regulators and Fiber Wall Biosynthesis

Lee

Zhuo

et al. 2019

Plant Physiol.

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“…In addition to the posttranslational regulation of NST1, it has been known for some time that other TFs and downstream SCW genes also undergo regulation via phosphorylation. This includes the phosphorylation of the TF E2Fc ( 50 ) and cellulose synthases (CESAs)1 and 7 in Arabidopsis ( 5 , 51 , 52 ), the phosphorylation of the MYB TF LTF1 in Populus ( 53 ), and the SUMOylation of the TF LBD30 in Arabidopsis ( 54 ). In a recent study, a quantitative atlas of the phosphoproteome of Arabidopsis was presented, and over 43,000 phosphorylation sites were found on 18,000 proteins ( 55 ).…”

Section: Discussionmentioning

confidence: 99%

Abscisic acid regulates secondary cell-wall formation and lignin deposition in Arabidopsis thaliana through phosphorylation of NST1

Liu

Rao

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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104

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Plant secondary cell-wall (SCW) deposition and lignification are affected by both seasonal factors and abiotic stress, and these responses may involve the hormone abscisic acid (ABA). However, the mechanisms involved are not clear. Here we show that mutations that limit ABA synthesis or signaling reduce the extent of SCW thickness and lignification in Arabidopsis thaliana through the core ABA-signaling pathway involving SnRK2 kinases. SnRK2.2. 3 and 6 physically interact with the SCW regulator NAC SECONDARY WALL THICKENING PROMOTING FACTOR 1 (NST1), a NAC family transcription factor that orchestrates the transcriptional activation of a suite of downstream SCW biosynthesis genes, some of which are involved in the biosynthesis of cellulose and lignin. This interaction leads to phosphorylation of NST1 at Ser316, a residue that is highly conserved among NST1 proteins from dicots, but not monocots, and is required for transcriptional activation of downstream SCW-related gene promoters. Loss of function of NST1 in the snd1 mutant background results in lack of SCWs in the interfascicular fiber region of the stem, and the Ser316Ala mutant of NST1 fails to complement this phenotype and ABA-induced lignin pathway gene expression. The discovery of NST1 as a key substrate for phosphorylation by SnRK2 suggests that the ABA-mediated core-signaling cascade provided land plants with a hormone-modulated, competitive desiccation-tolerance strategy allowing them to differentiate water-conducting and supporting tissues built of cells with thicker cell walls.

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“…In plants, a representative example for crosstalk of SUMOylation and ubiquitination involves the SUMOylation E3 ligase SIZ1 and the ubiquitination E3 ligase COP1 (CONSTITUTIVE PHOTOMORPHOGENIC 1) ( Figure 1 B). SIZ1 plays a role in multiple biological processes, such as phosphate deficiency responses, abiotic stress, ABA signaling, development, flowering time, and immunity ( Miura et al., 2005 , Catala et al., 2007 , Lee et al., 2007 , Jin and Hasegawa, 2008 , Miura et al., 2009 , Hammoudi et al., 2018 , Rytz et al., 2018 , Liu et al., 2019 , Niu et al., 2019 ). COP1 forms a complex with SPA1 (SUPPRESSOR OF PHYA-105 1) to function as an E3 ubiquitin ligase that acts as a key negative regulator of photomorphogenesis and plays an important role in plant immunity ( Lau and Deng, 2012 , Hofmann, 2015 , Zhu et al., 2015 , Hoecker, 2017 , Lim et al., 2018 ).…”

Section: Crosstalk Of Sumoylation and Ubiquitinationmentioning

confidence: 99%

Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

Zhang

Zeng

2020

Plant Communications

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Post-translational modifications (PTMs) are central to the modulation of protein activity, stability, subcellular localization, and interaction with partners. They greatly expand the diversity and functionality of the proteome and have taken the center stage as key players in regulating numerous cellular and physiological processes. Increasing evidence indicates that in addition to a single regulatory PTM, many proteins are modified by multiple different types of PTMs in an orchestrated manner to collectively modulate the biological outcome. Such PTM crosstalk creates a combinatorial explosion in the number of proteoforms in a cell and greatly improves the ability of plants to rapidly mount and fine-tune responses to different external and internal cues. While PTM crosstalk has been investigated in depth in humans, animals, and yeast, the study of interplay between different PTMs in plants is still at its infant stage. In the past decade, investigations showed that PTMs are widely involved and play critical roles in the regulation of interactions between plants and pathogens. In particular, ubiquitination has emerged as a key regulator of plant immunity. This review discusses recent studies of the crosstalk between ubiquitination and six other PTMs, i.e., phosphorylation, SUMOylation, poly(ADP-ribosyl)ation, acetylation, redox modification, and glycosylation, in the regulation of plant immunity. The two basic ways by which PTMs communicate as well as the underlying mechanisms and diverse outcomes of the PTM crosstalk in plant immunity are highlighted.

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SUMO modification of LBD30 by SIZ1 regulates secondary cell wall formation in Arabidopsis thaliana

Cited by 42 publications

References 74 publications

LBD29-Involved Auxin Signaling Represses NAC Master Regulators and Fiber Wall Biosynthesis

LBD29-Involved Auxin Signaling Represses NAC Master Regulators and Fiber Wall Biosynthesis

Abscisic acid regulates secondary cell-wall formation and lignin deposition in Arabidopsis thaliana through phosphorylation of NST1

Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

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