Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio

Ghate, NB; Das, Abhishek; Chaudhuri, Dipankar; Panja, Sourav; Mandal, Nripendranath

doi:10.1038/cddiscovery.2015.62

Cited by 35 publications

(25 citation statements)

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“…A large number of proteins operate in concert to regulate apoptosis. Bcl-2 is of particular interest as it has been reported to be protective against oxidative stress, reducing cell death induced by reactive oxygen species [78–80]. Previous studies have shown that cells overexpressing anti-apoptotic Bcl-2 exhibit a marked reduction in apoptosis, which suggests that anti-apoptotic Bcl-2 could independently affect the rate of apoptosis [81].…”

Section: Discussionmentioning

confidence: 99%

Acetaminophen attenuates lipopolysaccharide-induced cognitive impairment through antioxidant activity

Zhao

Zhang

Cao

et al. 2017

J Neuroinflammation

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BackgroundConsiderable evidence has shown that neuroinflammation and oxidative stress play an important role in the pathophysiology of postoperative cognitive dysfunction (POCD) and other progressive neurodegenerative disorders. Increasing evidence suggests that acetaminophen (APAP) has unappreciated antioxidant and anti-inflammatory properties. However, the impact of APAP on the cognitive sequelae of inflammatory and oxidative stress is unknown. The objective of this study is to explore whether APAP could have neuroprotective effects on lipopolysaccharide (LPS)-induced cognitive impairment in mice.MethodsA mouse model of LPS-induced cognitive impairment was established to evaluate the neuroprotective effects of APAP against LPS-induced cognitive impairment. Adult C57BL/6 mice were treated with APAP half an hour prior to intracerebroventricular microinjection of LPS and every day thereafter, until the end of the study period. The Morris water maze was used to assess cognitive function from postinjection days 1 to 3. Animal behavioural tests as well as pathological and biochemical assays were performed to evaluate LPS-induced hippocampal damage and the neuroprotective effect of APAP.ResultsMice treated with LPS exhibited impaired performance in the Morris water maze without changing spontaneous locomotor activity, which was ameliorated by treatment with APAP. APAP suppressed the accumulation of pro-inflammatory cytokines and microglial activation induced by LPS in the hippocampus. In addition, APAP increased SOD activity, reduced MDA levels, modulated glycogen synthase kinase 3β (GSK3β) activity and elevated brain-derived neurotrophic factor (BDNF) expression in the hippocampus. Moreover, APAP significantly decreased the Bax/Bcl-2 ratio and neuron apoptosis in the hippocampus of LPS-treated mice.ConclusionsOur results suggest that APAP may possess a neuroprotective effect against LPS-induced cognitive impairment and inflammatory and oxidative stress via mechanisms involving its antioxidant and anti-inflammatory properties, as well as its ability to inhibit the mitochondrial permeability transition (MPT) pore and the subsequent apoptotic pathway.

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Section: Discussionmentioning

confidence: 99%

Acetaminophen attenuates lipopolysaccharide-induced cognitive impairment through antioxidant activity

Zhao

Zhang

Cao

et al. 2017

J Neuroinflammation

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“…After 24 h, cells were subjected to compound 1 or 2 at the concentration of 50 μM for another 24 h. Cells then treated with LPS (500 ng/ml) (Sigma, MO) for 12 h. Subsequently, cells were harvested and extracted for analysis. The quantifification of mRNA in macrophage cells was performed using a previously standardized method 21,22 .…”

Section: Concise Synthesis Of Compounds 1 Andmentioning

confidence: 99%

Isolation of two rare N-glycosides from Ginkgo biloba and their anti-inflammatory activities

Cheng

Guo

Cui

et al. 2020

Sci Rep

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two rare N-β-D-glucopyranosyl-1H-indole-3-acetic acid conjugates, N-[2-(1-β-D-glucopyranosyl)-1Hindol-3-yl)acetyl]-L-glutamic acid (1) and N-[2-(1-β-D-glucopyranosyl)-1H-indol-3-yl)acetyl]-L-aspartic acid (2) were isolated from Ginkgo biloba. The structures were elucidated by analyses of HRMS and NMR spectroscopic data. In addition, a simplified and efficient synthetic route for compounds 1 and 2 is also disclosed to determine the absolute configurations of them. This concise syntheses of compounds 1 and 2 may facilitate studies of the biology of this type alkaloids. Compounds 1 and 2 were also tested for their cytotoxic and anti-inflammatory activities. The biological evaluation showed that compounds 1 and 2 led to the decrease of interleukin (IL)-6, nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 at mRNA level in lipopolysaccharide (LPS)-stimulated murine macrophage RAW264.7 cells. Ginkgo biloba L., one of the most well-known medicinal plants worldwide, is considered as a living fossil due to its survival over millions of years 1. Pharmacological studies have shown that extracts from its leaves and seeds exhibit antiparasitic, antifungal, antibacterial and antiviral activities. G. biloba extracts have also found broad applications in the treatment of cognitive diseases 2-5. Since the discovery of ginkgolides A-C, a lot of phytochemical and pharmaceutical studies have been conducted on the G. biloba leaves 6-8. It is well known that G. biloba leaf extracts, mainly composed of ginkgolides, flavonoids and phenolic compounds, are frequently used in the treatment of cardiovascular, cerebrovascular, and neurological diseases 9,10. The dried seeds of G. biloba (called "baiguo" in Chinese), have been reported in the Chinese Pharmacopoeia as effective treatments for cough, asthma, enuresis, alcohol misuse, pyogenic skin infections and worm infestations in the intestinal tract 11,12. In contrast to extensive studies on G. biloba leaves, the seeds of G. biloba have received much less attention. In order to find structurally interesting and bioactive components from Ginkgo seeds and provide a better understanding of their functions, we conducted a phytochemical investigation of Ginkgo seeds. As a result, two N-β-D-glucopyranosyl-1H-indole-3-acetic acid conjugates were isolated from the title plant and the concise syntheses of 1 and 2 were also enclosed to determine the absolute configurations of compounds 1 and 2 (Fig. 1). Notably, compounds 1 and 2 are naturally isolated N-glycosides, which are rarely found in natural products. To the best of our knowledge, only very few examples have been reported previouly 13-17. Although compounds 1 and 2 were detected in an alkaline hydrolysate of rice extract using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) 18 , there are no examples of their isolation, absolute configurations determination and bioactivities. Reported herein are the structural identification, concise syntheses and bioactivities of them. Results and discussion Ginkgos...

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“…77 Trans-9-hexadecen-1-ol was found in EARE (1.85%) and traces in EALE (0.30%), it shows anti-inflammatory and anticancer activity. 78 Quercitol or 1-deoxy Inositol is a cyclic polyalcohol found in MRE (2.26%) which works as a powerful osmolyte and helps in drought stress tolerance in plants. 79 Hydrocarbons 12 different hydrocarbon compounds were detected in different extracts of L. reticulata.…”

Section: Alcoholic and Phenolic Compoundsmentioning

confidence: 99%

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

Godara¹,

Dulara²,

Barwer³

et al. 2019

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The aim of this study is identification and comparative analysis of bioactive phytochemicals present in methanol and ethyl acetate extracts of different plant organs and callus of Leptadenia reticulata by GC-MS technique. Methodology: The shade dried plant samples (leaves stem and root) and callus produced from leaf of L. reticulata were powdered and then sequentially extracted in methanol and ethyl acetate solvents. Total eight extracts were prepared which were Methanolic Leaf Extract (MLE), Methanolic Stem Extract (MSE), Methanolic Root Extract (MRE), Methanolic Callus Extract (MCE), Ethyl Acetate Leaf Extract (EALE), Ethyl Acetate Root Extract (EARE), Ethyl Acetate Stem Extract (EASE) and Ethyl Acetate Callus Extract (EACE). Then, each of the extracts was further subjected to Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Results: The GC-MS analysis of the eight extracts revealed the presence of 77 different types of high and low molecular weight phytochemicals and bioactive compounds in varying quantities. Some of the phytochemicals detected for first time in L. reticulata are γ-sitosterol, Campesterol, Pristane, Hexahydrofarnesol, Stearic acid, Arachidic acid, Coniferyl alcohol, n-Tetracosanol-1, Ascorbic acid 2,6-dihexadecanoate, (2S,3S)-3,7,4'-Trihydroxy-5-methoxy-6-methylflavanone etc. These chemical compounds are considered biologically active and pharmacologically important. Conclusion: This study gives a detailed comparison of detection and identification of various bioactive phytochemicals from different plant parts (leaves, stem and root) and callus of L. reticulata. This provides a basis for the biological and biochemical characterization of some newly detected biologically and pharmacologically important phytochemical components from this plant.

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Sundew plant, a potential source of anti-inflammatory agents, selectively induces G2/M arrest and apoptosis in MCF-7 cells through upregulation of p53 and Bax/Bcl-2 ratio

Cited by 35 publications

References 31 publications

Acetaminophen attenuates lipopolysaccharide-induced cognitive impairment through antioxidant activity

Acetaminophen attenuates lipopolysaccharide-induced cognitive impairment through antioxidant activity

Isolation of two rare N-glycosides from Ginkgo biloba and their anti-inflammatory activities

Comparative GC-MS Analysis of Bioactive Phytochemicals from Different Plant Parts and Callus of Leptadenia reticulata Wight and Arn.

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