Sunxiuqinia dokdonensis sp. nov., isolated from deep sub-seafloor sediment

Abstract

A novel facultatively anaerobic strain DH1(T) was isolated from deep sub-seafloor sediment at a depth of 900 m below the seafloor off Seo-do (the west part of Dokdo Island) in the East Sea of the Republic of Korea. The new strain was characterized using polyphasic approaches. The isolate was Gram-stain-negative, motile by gliding, non-spore-forming rods, oxidase-negative, and catalase-positive; and formed colonies of orange-red color. The NaCl range for growth was 0.5-7.0% (w/v) and no growth was observed in the absence of NaCl. The isolate grew optimally at 30°C, with 2% (w/v) NaCl and at pH 7. The cell-wall hydrolysates contained ribose as a major sugar. The DNA G+C content was 40.8 mol%. The closest related strains are Sunxiuqinia faeciviva JAM-BA0302(T) and Sunxiuqinia elliptica DQHS-4(T) (97.9 and 96.3% sequence similarity, respectively). The level of DNA-DNA relatedness between strain DH1(T) and S. faeciviva JAM-BA0302(T) was around 41% (but only 6% between DH1T and S. elliptica DQHS-4(T)). The major cellular fatty acids of the isolate were contained iso-C15:0 (25.9%), anteiso-C15:0 (16.7%), and summed feature 9 (comprising C16:0 3-OH and/or unknown fatty acid of dimethylacetal ECL 17.157; 13.2%). The predominant menaquinone was MK-7. On the basis of polyphasic evidence from this study, the isolate was considered to represent a novel species of the genus Sunxiuqinia, for which the name Sunxiuqinia dokdonensis sp. nov. is proposed; the type strain is DH1(T) (=KCTC 32503(T) =CGMCC 1.12676(T) =JCM 19380(T)).

Microbial iron reduction and methane oxidation in subsurface sediments of the Arabian Sea

“…Facultative anaerobes have been isolated from a depth of 900mbsf in sediments of Sea-do located towards the western part of Dokdo Island in the East Sea of Republic of Korea (Chang et al, 2013). In addition, the present study also showed that there was no significant correlation between TVCa and TVCan indicating that the overlap between these two groups was less, possibly suggesting an existence of physiologically different communities.…”

Whole-genome sequence of Sunxiuqinia dokdonensis DH1 T , isolated from deep sub-seafloor sediment in Dokdo Island

“…Among them, S. elliptica and S. rutile were isolated from shallow sea sediment in a sea cucumber farm and at Nagasuka Fishery Harbor, respectively. In contrast, S. dokdonensis and S. faeciviva were isolated from deep sub-seafloor sediment at a depth of 900 m below the seafloor off the west part of Dokdo Island and at a depth of 247.1 m below the seafloor off the Shimokita Peninsula, respectively [4] , [5] , [6] , [7] . Based on difference of sampling depth, these two groups were clearly separated through the phylogenetic tree ( Fig.…”

Deltaproteobacterium Strain KaireiS1, a Mesophilic, Hydrogen-Oxidizing and Sulfate-Reducing Bacterium From an Inactive Deep-Sea Hydrothermal Chimney

“…Although several dilutions were performed, the 16S rRNA gene of Sunxiuqinia species was still detectable in the organic amended Desulfobulbus enrichments. The genus Sunxiuqinia is classified as a member of the Bacteroidetes , comprising heterotrophic, aerobic, or facultative aerobic species isolated from coastal marine sediments and deep sub-seafloor sediments ( Qu et al, 2011 ; Chang et al, 2013 ; Takai et al, 2013 ; Yoon and Kasai, 2014 ). Among the Desulfobulbaceae family, a co-culture has been reported of the host-associated Desulfobulbus oralis and Fusobacterium nucleatum .…”