¹H HR-MAS NMR and S180 Cells: Metabolite Assignment and Evaluation of Pulse Sequence

Integrating High-Resolution and Solid-State Magic Angle Spinning NMR Spectroscopy and a Transcriptomic Analysis of Soybean Tissues in Response to Water Deficiency

“…Cross polarisation and magic angle spinning (CP‐MAS) techniques have been extensively used to investigate the cell wall and protective tissues (Dick‐Perez et al , ; Serra et al , ). In a complementary fashion, high‐resolution magic angle spinning (HR‐MAS) has been successfully applied to analyse the soluble metabolites in intact cells and tissues from many organs, such as brain, lung, kidney, heart and muscle (Oliveira et al , ), fragile tissues (André et al , ), and for the analysis a wide range of samples, such as fish, lamb, beef, cheese, grains, fruits and vegetables (Santos et al , ). HR‐MAS NMR combines the typical advantages of liquid‐state NMR and SSNMR, such as short acquisition times for 1 H–NMR spectra, even from a few milligrams of sample.…”

1H HR-MAS NMR-Based Metabolomic Fingerprinting to Distinguish Morphological Similarities and Metabolic Profiles of Maytenus ilicifolia, a Brazilian Medicinal Plant

“…These influential interactions could be observed by imperfect signal resolution (line-broadening) and spin (transverse T 2 ) relaxations, although water disrupts signal digitization ( S/N loss) of anticipated molecules with further artifacts in the signal. These undesirable trends may be manipulated using HR-MAS NMR by providing a spinning speed of 100–1000 Hz to the sample in the MAS rotor in the magic angle direction (θ MAS = 54.74°) to B 0 . ,, However, the substantial contribution of water in the sample can be blocked by utilizing a specific pulse sequence ( noesypr1d , among others) in the NMR experiment …”