<sup>1</sup>H NMR spectroscopy in the diagnosis of <i>Pseudomonas aeruginosa</i>‐induced urinary tract infection

Gupta, Ashish; Dwivedi, Manish Kumar; Gowda, G. A. Nagana; Ayyagarí, A; Mahdi, Abbas Ali; Bhandari, Mahendra; Khetrapal, C. L.

doi:10.1002/nbm.957

Cited by 34 publications

(28 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, tyrosine and valine were only excreted into LB and not in SCFM. Finally, the increase of 6-hydroxynicotinic acid in P. aeruginosa cultures is probably due to limited catabolism of NAD or niacin and has been used as a diagnostic marker of P. aeruginosa infection (20).…”

Section: Discussionmentioning

confidence: 99%

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Behrends

Ebbels

Williams

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

Untargeted profiling of small-molecule metabolites from microbial culture supernatants (metabolic footprinting) has great potential as a phenotyping tool. We used time-resolved metabolic footprinting to compare one Escherichia coli and three Pseudomonas aeruginosa strains growing on complex media and show that considering metabolite changes over the whole course of growth provides much more information than analyses based on data from a single time point. Most strikingly, there was pronounced selectivity in metabolite uptake, even when the bacteria were growing apparently exponentially, with certain groups of metabolites not taken up until others had been entirely depleted from the medium. In addition, metabolite excretion showed some complex patterns. Fitting nonlinear equations (four-parameter sigmoids) to individual metabolite data allowed us to model these changes for metabolite uptake and visualize them by back-projecting the curve-fit parameters onto the original growth curves. These "uptake window" plots clearly demonstrated strain differences, with the uptake of some compounds being reversed in order between different strains. Comparison of an undefined rich medium with a defined complex medium designed to mimic cystic fibrosis sputum showed many differences, both qualitative and quantitative, with a greater proportion of excreted to utilized metabolites in the defined medium. Extending the strain comparison to a more closely related set of isolates showed that it was possible to discriminate two species of the Burkholderia cepacia complex based on uptake dynamics alone. We believe time-resolved metabolic footprinting could be a valuable tool for many questions in bacteriology, including isolate comparisons, phenotyping deletion mutants, and as a functional complement to taxonomic classifications.

show abstract

Section: Discussionmentioning

confidence: 99%

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Behrends

Ebbels

Williams

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Moreover, recently Gupta et al, using the 1 H NMR metabolic approach, identified and quantified E. coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis isolates in urine samples from patients with urinary tract infections (UTIs) faster than using culture methods. This NMR method of metabolic profile exploration in urine provided an alternative approach for screening and identification of UTI (31)(32)(33)(34). These perspectives, along with the study reported in this paper, suggest the great potential of 1 H NMR for antimicrobial analysis.…”

Section: Discussionmentioning

confidence: 69%

Proton Nuclear Magnetic Resonance Spectroscopy as a Technique for Gentamicin Drug Susceptibility Studies with Escherichia coli ATCC 25922

et al. 2015

View full text Add to dashboard Cite

Antimicrobial drug susceptibility tests involving multiple time-consuming steps are still used as reference methods. Today, there is a need for the development of new automated instruments that can provide faster results and reduce operating time, reagent costs, and labor requirements. Nuclear magnetic resonance (NMR) spectroscopy meets those requirements. The metabolism and antimicrobial susceptibility of Escherichia coli ATCC 25922 in the presence of gentamicin have been analyzed using NMR and compared with a reference method. Direct incubation of the bacteria (with and without gentamicin) into the NMR tube has also been performed, and differences in the NMR spectra were obtained. The MIC, determined by the reference method found in this study, would correspond with the termination of the bacterial metabolism observed with NMR. Experiments carried out directly into the NMR tube enabled the development of antimicrobial drug susceptibility tests to assess the effectiveness of the antibiotic. NMR is an objective and reproducible method for showing the effects of a drug on the subject bacterium and can emerge as an excellent tool for studying bacterial activity in the presence of different antibiotic concentrations. Infectious diseases remain a challenge in terms of mortality and morbidity despite the great progress achieved in public health in recent decades. For this reason, antimicrobial susceptibility evaluations and new antibiotic screenings are a field of great importance (1). Antimicrobial susceptibility testing (AST) studies are crucial to determine the most effective drug available for the treatment of a patient with an infectious disease process. Currently, automated in vitro AST systems are used with standardized methods and conditions to determine the MIC of a drug. These tests usually function by visually judging liquid turbidity, and are one of the reference methods still used today. Unfortunately, these tests involve multiple time-consuming steps, which are often slow, laborious, and susceptible to testing sensitivity problems (2, 3). Moreover, these methods should be useful against pathogens whose susceptibility to drugs is not predictable, thus enabling researchers to determine the antimicrobial susceptibility of fastidious organisms (4) and of those microorganisms that have acquired resistance mechanisms. Measuring the susceptibility of microorganisms to antimicrobials began in the early 1920s (5). Subsequently, there was recognition (as early as the late 1950s) that required the standardization of these techniques. Therefore, numerous AST methods have been described (6). However, there is currently a need for the development of new automated instruments that can provide faster results, reduce reagent cost, and minimize labor requirements (7,8). In this context, several methods were recently established to minimize the time required to complete the test and the number of operation steps (9, 10).Nuclear magnetic resonance (NMR) spectroscopy has been used as a method to determine structures of synt...

show abstract

“…After another 30 min of incubation, uroepithelial cells were washed with 50 ml PBS. Bacterial cells in washed PBS were counted by standard pour plate method [17][18][19].…”

Section: Hemagglutination Assaymentioning

confidence: 99%

“…For this 500 ll of the bacterial suspension and 500 ll of normal saline (in place of proanthocyanidin solution) were mixed and inoculated in a cell culture flask. After 30 min of incubation, uroepithelial cells were washed; bacterial cells in the washed PBS were counted, as described previously [17][18][19].…”

Section: Hemagglutination Assaymentioning

confidence: 99%

Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

et al. 2011

Self Cite

View full text Add to dashboard Cite

Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13 C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 lg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

show abstract

¹H NMR spectroscopy in the diagnosis of Pseudomonas aeruginosa‐induced urinary tract infection

Cited by 34 publications

References 15 publications

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Proton Nuclear Magnetic Resonance Spectroscopy as a Technique for Gentamicin Drug Susceptibility Studies with Escherichia coli ATCC 25922

Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

Contact Info

Product

Resources

About

1H NMR spectroscopy in the diagnosis of Pseudomonas aeruginosa‐induced urinary tract infection

Cited by 34 publications

References 15 publications

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Time-Resolved Metabolic Footprinting for Nonlinear Modeling of Bacterial Substrate Utilization

Proton Nuclear Magnetic Resonance Spectroscopy as a Technique for Gentamicin Drug Susceptibility Studies with Escherichia coli ATCC 25922

Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

Contact Info

Product

Resources

About

¹H NMR spectroscopy in the diagnosis of Pseudomonas aeruginosa‐induced urinary tract infection