¹⁹F Magic Angle Spinning Dynamic Nuclear Polarization Enhanced NMR Spectroscopy

Abstract

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19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies

“…This requires the preparation of several samples with a varying number of 19 F sites per molecule and/or per CA tube (e.g., by mixing the 19 F-labeled and nonfluorinated CA in different ratios prior to assembly) and/or the introduction of covalently linked biradical tags in close proximity to one of the 19 F sites such that the polarization transfer originates on a particular fluorine site and then propagates to the other sites through spin diffusion. Larger enhancements and shorter buildup times could potentially be obtained by the addition of fluorinated molecules in the polarization matrix, according to a recent study, although this may be difficult to achieve in practice for aqueous protein preparations.…”

High Sensitivity Detection of a Solubility Limiting Surface Transformation of Drug Particles by DNP SENS

“…26 It has also been shown how hyperpolarized 19 F NMR can be used to detect low quantities (2 mmol) of fluorinated APIs. 27 Our group has exploited 1 He 1 H spin dynamics in DNP experiments to numerically simulate polarization transfer, using a thermal transfer model, which can then be used to extract domain sizes. 28,29 This has been demonstrated by measuring distances in microcrystalline solids, 29 APIs, 23 and polymers.…”