[³H]Inulin as a Marker for Glomerular Filtration Rate

MultiSig: a new high-precision approach to the analysis of complex biomolecular systems

“…The overall shape of the two distributions is similar: both show a component having a narrow distribution (σ = 1.65, s 20,b = 0.255S) and a series of lower σ / s value minor components, which may well be degradation products (Windfield et al 2003 ). There is no valid way in which the bi- (or possibly tri-) modal c ( s ) distribution (right) can be transformed to yield even the relative masses of the two components, whereas with an estimate (0.60 ml/g) for the partial specific volume of the solute available, the (average) absolute molecular weights of the two species can be defined from the σ values associated with the peaks and their relative concentrations obtained even without that knowledge.…”

“…Inulins, widely studied and used, usually have a major component with a molecular weight in the region of 5–6 kDa, sedimentation coefficient s 20,w ~0.7S (Imran et al 2012 ; Azis et al 1999 ). They readily degrade to smaller species, particularly at acidic pH levels (Windfield et al 2003 ). The value for the molecular weight of the (higher molecular weight, putatively intact) component is 5.9 kDa—within the accepted range quoted—and for the two lower weight species identified (Fig.…”

Combined prenatal and postnatal protein restriction influences adult kidney structure, function, and arterial pressure

“…Nevertheless, the GFR measurements in our present study should probably be considered only as preliminary data and interpreted with caution. There is considerable controversy regarding the validity of methods for subacute and chronic measurement of GFR in rodents, particularly for methods using [ 3 H]inulin clearance (30). Therefore, further studies are required to confirm or deny the hypothesis that protein restriction predisposes to hyperfiltration in response to increased salt intake.…”

Development and validation of an HPLC-UV method for determination of iohexol in human plasma

“…The "Gold Standard" for determining GFR is to measure the clearance of an exogenous substance such as inulin, a polyfructose molecule with an average molecular weight of 5200 Da. Because of the lack of commercially available dosage forms, need for an infusion pump and multiple sample collection, GFR measurement using inulin becomes expensive and its reliability dependent on the method used for the analysis of the polyfructose molecule [1]. As an alternative to inulin, two major classes of agents have been introduced-the radio labeled chelating agents including ethylene diamine tetraacetic acid ( 51 Cr-EDTA) or diethylene triamine penta-acetic acid ( 99m Tc-DTPA) and radiological contrast media agents (including 125 I-iothalamate) [2].…”