<sup>31</sup>P NMR measurements of the effects of unsaturated fatty acids on cellular phospholipid metabolism

Gillham, Helen; Brindle, Kevin M.

doi:10.1002/mrm.1910350406

Cited by 9 publications

(8 citation statements)

References 38 publications

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“…33 It is interesting to note that in cultured glioma cells, the ratio of phosphoethanolamine to phosphocholine increases as the cells approach confluence. 32 While cell preparations have the advantage that a single population of cells can be studied in a defined environment, the results obtained must be interpreted cautiously, since phosphoethanolamine and phosphocholine levels depend critically on the relative levels of ethanolamine, choline and serine in the medium, 28,32 as well as on such factors as pH, glucose concentration, 34 fatty acid concentrations 35 and passage number. 36 For these reasons, and despite the greater complexity of the models, tumours growing in vivo will probably always be necessary for a fuller understanding of factors influencing tumour cell metabolism.…”

Section: Discussionmentioning

confidence: 99%

NMR studies of phospholipid metabolism in hepatic lymphoma

Dixon

1998

NMR Biomed.

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The detection of lymphomatous infiltration of the liver has implications for the staging and treatment of this disease. Our studies of patients with Hodgkin's disease and non‐Hodgkin's lymphoma suggest that the involvement of the liver could be detected by 31P nuclear magnetic resonance (NMR) spectroscopy as an increase in the phosphomonoester/ATP and phosphomonoester/Pi ratios in the liver spectra in vivo. Studies of extracts of lymphomatous lymph nodes and of the lymphomatous mouse liver, showed that phosphoethanolamine was largely responsible for the increase in the phosphomonoester (PME) signal. This compound is involved in phospholipid metabolism, as a precursor and breakdown product of phosphatidylethanolamine. The kinetics of the synthesis of phosphatidylethanolamine from [13C2]ethanolamine were studied using 13C NMR spectroscopy. The increase in phosphoethanolamine in the lymphomatous liver was not found to be due to increased flux through the synthetic pathway to phosphatidylethanolamine, nor was it due to increased availability of ethanolamine. © 1998 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

NMR studies of phospholipid metabolism in hepatic lymphoma

Dixon

1998

NMR Biomed.

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“…In HL-60 cells, there was an increase in PE and a decrease in PC concentrations after the induction of PCD. We have observed elevated PE levels previously in cells treated with unsaturated fatty acids, in which the increases were attributed to activation of phospholipases C and D (59).…”

Section: Effects On Phospholipid Metabolismmentioning

confidence: 92%

Induction of apoptosis in two mammalian cell lines results in increased levels of fructose‐1,6‐Bisphosphate and CDP‐choline as determined by ³¹P MRS

Williams

Anthony

Brindle

1998

Magnetic Resonance in Med

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Programmed cell death or apoptosis was induced in human promyelocytic leukemia (HL-60) and Chinese hamster ovary (CHO-K1) cells using several cytotoxic drugs that have different modes of action, including camptothecin, ceramide, chelerythrine, etoposide, farnesol, geranyl geraniol, and hexadecylphosphocholine. The consequent changes in cellular metabolism were monitored using 31P MRS measurements on intact cells and cell extracts. Cells undergoing programmed cell death exhibited characteristic changes in the levels of glycolytic and phospholipid metabolites. The most significant changes were increases in the concentration of the glycolytic intermediate, fructose-1,6-bisphosphate and in the concentration of CDP-choline, which is an intermediate in phosphatidylcholine biosynthesis. In HL-60 cells, the increase in fructose-1,6-bisphosphate levels could be explained by depletion of cellular NAD(H) levels. All of the agents used to induce apoptosis caused the accumulation of CDP-choline. Since the resonances of this compound occur in a relatively well resolved region of tissue spectra, it could provide a marker for apoptosis that would allow the noninvasive detection of the process in vivo using 31P MRS measurements.

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“…Although the mechanism and significance for elevated concentration of PE in tumors are not fully understood, it is likely related to the activation of phosphatidylethanolamine metabolism that plays a key role in the modification of the tumor cell membrane (50). A high concentration of PE has also been observed with stimulation of phospholipase C or D in a variety of cancer cell lines (51, 52). The enzymes phospholipase C or D trigger the breakdown of phosphatidylethanolamine to PE and diacylglycerol, and PE acts as a long-term second-messenger system for cellular proliferation and cell growth (53).…”

Section: Discussionmentioning

confidence: 98%

Proton and Phosphorous MR Spectroscopy in Squamous Cell Carcinomas of the Head and Neck

et al. 2009

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Rationale and objectives-Phosphorous magnetic resonance spectroscopy ( 31 P MRS) has been used to evaluate and predict treatment response in squamous cell carcinomas of the head and neck (HNSCC). Several studies have also shown the potential of proton MR spectroscopy ( 1 H MRS) in assessing response in HNSCC. In view of the inherent limitations associated with performing 31 P MRS in clinical settings, the current study was performed to explore as to whether 1 H MRS could provide similar or complimentary metabolic information in HNSCC.Materials and Methods-Fifteen patients with HNSCC underwent pretreatment MR imaging. Both 1 H MRS and 31 P MRS were performed on viable and solid parts of the metastatic lymph nodes of these patients. Peak areas of total choline (tCho) and unsuppressed water, as observed on 1 H MRS and phosphomonoester (PME) and β-nucleotide triphosphates (β-NTPs) from 31 P MRS were computed. The Pearson correlation coefficient value was used to correlate tCho/water and PME/β-NTP ratios.Results-In all the patients, the metastatic nodes appeared hyperintense on T2 weighted images and hypointense on T1 weighted images with variable signal intensity. A prominent resonance of tCho on 1 H MRS and a resonance of PME on 31 P MRS from metastatic nodes of all patients were observed. A moderate correlation coefficient of 0.31 was observed between tCho/water and PME/ β-NTP (p>0.05). Conclusion-The biochemical pathways involved in 1 H MRS of tCho may be different from the phospholipids metabolites seen by 31 P MRS of head and neck cancers and thus the two MRS techniques may be complimentary to each other.

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³¹P NMR measurements of the effects of unsaturated fatty acids on cellular phospholipid metabolism

Cited by 9 publications

References 38 publications

NMR studies of phospholipid metabolism in hepatic lymphoma

NMR studies of phospholipid metabolism in hepatic lymphoma

Induction of apoptosis in two mammalian cell lines results in increased levels of fructose‐1,6‐Bisphosphate and CDP‐choline as determined by ³¹P MRS

Proton and Phosphorous MR Spectroscopy in Squamous Cell Carcinomas of the Head and Neck

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31P NMR measurements of the effects of unsaturated fatty acids on cellular phospholipid metabolism

Cited by 9 publications

References 38 publications

NMR studies of phospholipid metabolism in hepatic lymphoma

NMR studies of phospholipid metabolism in hepatic lymphoma

Induction of apoptosis in two mammalian cell lines results in increased levels of fructose‐1,6‐Bisphosphate and CDP‐choline as determined by 31P MRS

Proton and Phosphorous MR Spectroscopy in Squamous Cell Carcinomas of the Head and Neck

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³¹P NMR measurements of the effects of unsaturated fatty acids on cellular phospholipid metabolism

Induction of apoptosis in two mammalian cell lines results in increased levels of fructose‐1,6‐Bisphosphate and CDP‐choline as determined by ³¹P MRS