NMR studies of phospholipid metabolism in hepatic lymphoma

Abstract

The detection of lymphomatous infiltration of the liver has implications for the staging and treatment of this disease. Our studies of patients with Hodgkin's disease and non‐Hodgkin's lymphoma suggest that the involvement of the liver could be detected by 31P nuclear magnetic resonance (NMR) spectroscopy as an increase in the phosphomonoester/ATP and phosphomonoester/Pi ratios in the liver spectra in vivo. Studies of extracts of lymphomatous lymph nodes and of the lymphomatous mouse liver, showed that phosphoethanolamine was largely responsible for the increase in the phosphomonoester (PME) signal. This compound is involved in phospholipid metabolism, as a precursor and breakdown product of phosphatidylethanolamine. The kinetics of the synthesis of phosphatidylethanolamine from [13C2]ethanolamine were studied using 13C NMR spectroscopy. The increase in phosphoethanolamine in the lymphomatous liver was not found to be due to increased flux through the synthetic pathway to phosphatidylethanolamine, nor was it due to increased availability of ethanolamine. © 1998 John Wiley & Sons, Ltd.

Quantitative proton magnetic resonance spectroscopy of the normal liver and malignant hepatic lesions at 3.0 Tesla

“…In addition, 1 H MRS allowed one to determine the methyl (CH 3 ) and methylene (-CH 2 ) n protons of fatty acid chains. The signals of the corresponding lipid groups can be observed at 1.2 ppm for (-CH 2 ) n and 0.9 ppm for (CH 3 Table 2.…”

“…31 P magnetic resonance spectroscopy (MRS) has been used in other studies to differentiate normal liver tissue from hepatic lesions. 31 P MR spectra obtained from tumour-bearing livers showed significant elevations of the phosphomonoester resonance (phosphoethanolamine and phosphocholine resonances) compared with normal liver, which was attributed to increased cell turnover [2,3].…”

In vivo detection of metabolic changes by 1 H-MRS in the DEN-induced hepatocellular carcinoma in wistar rat

“…In the hepatic field, some diffuse hepatic disease, such as chronic hepatitis (Cho et al 2001;Taylor-Robinson et al 1997), and cirrhosis (Lim et al 2003), has been tested by MRS and proven to be useful. Phosphorus-31 MRS has been used to assess hepatocellular adenomas (Lim et al 2002) and hepatic lymphoma (Dixon 1998). It has been reported that the lipid components in a chemical-induced rat HCC have some changes (Foley et al 2001).…”

Application of Single Voxel 1H Magnetic Resonance Spectroscopy in Hepatic Benign and Malignant Lesions

“…An intro 31 P MRS experiment by Dixon et al showed that Cho compounds were largely responsible for the increase in the phosphomonoester (PME) signal in hepatic lesions [26]. Another study also demonstrated that primary hepatocellular carcinoma showed obvious higher Cho values than cirrhotic and normal liver tissues and suggested that the values of Cho peaks can be used as an index of tumor cell proliferation [20].…”