³¹P NMR spectroscopy of isolated perfused lungs

Abstract

31P NMR spectra of isolated blood-perfused pig lungs were obtained by degassing the lungs in vivo to remove field inhomogeneities caused by air-tissue interfaces. The spectra show the presence of ATP, phosphodiester, inorganic phosphate, and phosphomonoester, but no phosphocreatine. All the metabolites remained stable for more than 4 h when the lungs were perfused with oxygenated blood. Blood gas tensions, glucose concentration, pH, and temperature were controlled throughout the experiment. During anoxia or ischemia, ATP and intracellular pH declined and Pi increased but returned to control levels during subsequent normoxia or reperfusion. These results demonstrate the applicability of NMR spectroscopy to isolated perfused lungs, enabling studies of metabolic processes in normal and pathologic lungs, as well as establishment of optimal conditions for lung preservation for transplantation.

Hydrogen ion currents in rat alveolar epithelial cells

“…Lonnerholm, 1982). The pHi of pig lungs drops from 7.47 to 7.11 or 6.37, after 15 min of anoxia or ischemia, respectively (Pillai et al, 1986). It can be imagined that if the gH were activated by low pHi, this mechanism could allow rapid dissipation of a large acid load.…”

Modeling the nuclear magnetic resonance behavior of lung: From electrical engineering to critical care medicine

“…As mentioned above, the FID has been found to be much more rapid in inflated excised rat lungs than in airless (vacuum degassed) lungs [Morris et al, 1985]. The NMR line-broadening associated with inflation has been observed in rat, pig, and human lungs [Pillai et al, 1986;Bergin et al, 1991;Ganesan et al, 1993].…”

Hypoxic and Complement-Induced Lung Injury in the Isolated Perfused Rat Lung as Determined by Phosphorous-31 Nuclear Magnetic Resonance

“…Also, abnormalities in lung hypoxia have been described in vivo in the isolated pigs lung [6]. Studies of lung edema with MRS have also proven useful [7].…”