Super-Resolution Microscopy and Their Applications in Food Materials: Beyond the Resolution Limits of Fluorescence Microscopy

Gallegos-Cerda, Susana Dianey; Hernández‐Varela, Josué David; Chanona-Pérez, Jorge; Arredondo-Tamayo, Benjamín; Méndez, Juan Vicente Méndez

doi:10.1007/s11947-022-02883-4

Cited by 7 publications

(1 citation statement)

References 124 publications

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“…[ 9 ] Concretely, MINFLUX separates neighboring fluorophores by on/off‐switching their emission capability individually, like PALM or STORM, but localizes the emitting fluorophore with a pattern of excitation light featuring an intensity node, like the donut used in STED microscopy. [ 10 ] Localization with a donut‐shaped excitation beam shifts the burden of requiring many photons for localization on the excitation beam, so that the number of fluorescence photons required for attaining a certain localization precision or speed is substantially reduced. [ 9 ] Minimizing the required number of detected photons lessens the demand on the on/off‐switchable fluorophores regarding brightness in the “on”‐state and photostability.…”

Section: Introductionmentioning

confidence: 99%

Accelerated MINFLUX Nanoscopy, through Spontaneously Fast‐Blinking Fluorophores

Remmel

Scheiderer

Butkevich³

et al. 2023

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The introduction of MINFLUX nanoscopy allows single molecules to be localized with one nanometer precision in as little as one millisecond. However, current applications have so far focused on increasing this precision by optimizing photon collection, rather than minimizing the localization time. Concurrently, commonly used fluorescent switches are specifically designed for stochastic methods (e.g., STORM), optimized for a high photon yield and rather long on‐times (tens of milliseconds). Here, accelerated MINFLUX nanoscopy with up to a 30‐fold gain in localization speed is presented. The improvement is attained by designing spontaneously blinking fluorescent markers with remarkably fast on‐times, down to 1–3 ms, matching the iterative localization process used in a MINFLUX microscope. This design utilizes a silicon rhodamine amide core, shifting the spirocyclization equilibrium toward an uncharged closed form at physiological conditions and imparting intact live cell permeability, modified with a fused (benzo)thiophene spirolactam fragment. The best candidate for MINFLUX microscopy (also suitable for STORM imaging) is selected through detailed characterization of the blinking behavior of single fluorophores, bound to different protein tags. Finally, optimization of the localization routines, customized to the fast blinking times, renders a significant speed improvement on a commercial MINFLUX microscope.

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Section: Introductionmentioning

confidence: 99%

Accelerated MINFLUX Nanoscopy, through Spontaneously Fast‐Blinking Fluorophores

Remmel

Scheiderer

Butkevich³

et al. 2023

Small

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Assessing the reinforcement effect by response surface methodology of holocellulose from spent coffee grounds on biopolymeric films as food packaging materials

Hernández‐Varela,

Chanona‐Pérez,

Foruzanmehr

et al. 2024

Biopolymers

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The pollution caused by petroleum‐derived plastic materials has become a major environmental problem that has encouraged the development of new compostable and environmentally friendly materials for food packaging based on biomodified polymers with household residues. This study aims to design, synthesize, and characterize a biobased polymeric microstructure film from polyvinyl alcohol and chitosan reinforced with holocellulose from spent coffee grounds for food‐sustainable packaging. Chemical isolation with a chlorite‐based solution was performed to obtain the reinforced holocellulose from the spent coffee ground, and the solvent casting method was used to obtain the films to study. Physicochemical and microscopic characterizations were conducted to identify and select the best formulations using a simplex‐centroid design analysis. The response surface methodology results indicate that the new packaging material obtained with equal amounts of polymers and reinforced material (1:1:1) possesses the appropriate barrier properties and microstructural character to prevent water attack and hydrophobic behavior and thus could be used as an alternative for food packaging materials.

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