Supercritical carbon dioxide extraction and fractionation of lipids from freeze-dried microalgae Nannochloropsis oculata and Chlorella vulgaris

Obeid, Sara; Beaufils, Nicolas; Camy, Séverine; Takache, Hosni; Ismail, Ali; Pontalier, Pierre-Yves

doi:10.1016/j.algal.2018.07.003

Cited by 91 publications

(22 citation statements)

References 34 publications

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“…For N. oculata cells, our results are in contrast to those by Ryckebosch et al (2014), who reported that standard cultures of the alga contained about 40% of neutral lipids. Hovewer, our data are in agreement with other papers using the alga under stress conditions (Obeid et al 2018). In our experiments, the stress conditions could be the age of culture in mono-cultivation and both the age of the culture and other stresses, like hydrodynamic stress or interaction with mucopolysaccharides released by T. lutea.…”

Section: Lipid and Fatty Acid Profilesupporting

confidence: 92%

A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

et al. 2021

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The biotechnological potential of microalgae has gained considerable importance in many applied fields: biomass production for food and feed, cosmeceutical and pharmaceutical products, energy and phytoremediation. The driving force that inspires the progress in microalgae production is the need for new cultivation systems to obtain simultaneously the maximum yield, reduction of water and nutrients use, and production of economically interesting molecules, such as pigments, fatty acids and polysaccharides. We aim to test, for the first time, the co-cultivation in saline medium of Tisochrysis lutea (Haptophyta) and Nannochloropsis oculata (Ochrophyta) to obtain valuable compounds, i.e. pigments and lipids characteristic of each species, using a single culture process. Mono-cultures of each strain were used as controls. The two strains showed an increase in the concentration of chlorophylls and carotenoids in co-culture. At the end of the experiment, the fatty acid profile was analysed by gas chromatography–mass spectrometry. The lipids in the co-cultivated cell extracts were mainly attributable to N. oculata, which represented 97% of the total cells (ca. 83% of the total biomass) at the end of the experiment. Nevertheless, the ω-3 characteristic of T. lutea (DHA and SDA, absent in N. oculata) was also detectable. Although the co-cultivation of these two phylogenetically different species of microalgae did not show positive effects on the growth and on the total lipid production, however, this process resulted in a reduction of the production costs and a lower consumption of water and nutrients.

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Section: Lipid and Fatty Acid Profilesupporting

confidence: 92%

A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

et al. 2021

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“…Cell disruption can be achieved by physical (Ultrasonication, High Pressure Homogenization, Bead Milling, Pulsed Electric Field) chemical (Acid, Alkaline and Oxidation), thermal (Hydrothermal, Steam Explosion) and biological methods (Enzymatic treatment) (Soo Youn . During cell wall disruption, intracellular molecules can be extracted with organic solvents (Araujo et al, 2013), ionic liquids (Y. , supercritical CO2 (Reyes et al, 2016;Cheng et al, 2011) or supercritical mixtures of CO2 and solvents (Obeid et al, 2018). Cell-wall components fractionation, may require their further depolymerization via hydrothermal (Lorente et al, 2017), acid (Hernández et al, 2015) or enzymatic hydrolysis (Sierra et al, 2017).…”

Section: Microalgae In the Context Of Biorefinerymentioning

confidence: 99%

Current and novel approaches to downstream processing of microalgae: A review

Nitsos

Filali

Taidi

et al. 2020

Biotechnology Advances

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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“…The most common supercritical solvent is carbon dioxide (104, 105) but acetone, methanol, ethanol, and water also used for lipid extraction under supercritical conditions (103,106). Supercritical carbon-dioxide efficiently solubilize non-polar compounds while the extraction polar compounds can be increased using a polar co-solvent as ethanol, methanol, and acetyl chloride (107) or azeotropic mixtures as hexane/ ethanol (108). The parameters of SFE, such as extraction temperature, extraction time, biomass/solvent ratio, and pressure significantly influence the quality of extracted oil and obtained biodiesel (109,110).…”

Section: Supercritical Fluid Extractionmentioning

confidence: 99%

The advances and limitations in biodiesel production: feedstocks, oil extraction methods, production, and environmental life cycle assessment

Pikula

Zakharenko

Stratidakis

et al. 2020

Green Chemistry Letters and Reviews

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Although fossil fuels remain the main source of energy, the volume of renewable sources of energy is constantly increasing. Biodiesel is a promising alternative fuel due to the number of advantages compared to fossil fuel and other types of biofuel. The specific objective of this study was to identify the difference between conventional and novel technologies applied during the whole life cycle of biodiesel production and consumption. The study offers some important insights into the recent advances in the biodiesel industry including biodiesel production from microalgal lipids, advanced homogenous and enzymatic transesterification, non-catalytic supercritical transesterification, application of microwave and ultrasound assisting technologies. Considering all the factors affecting the efficiency and safety of the biodiesel production process, here we reviewed the main principals and recent achievements in the environmental life cycle assessment of biodiesel production and consumption.

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Supercritical carbon dioxide extraction and fractionation of lipids from freeze-dried microalgae Nannochloropsis oculata and Chlorella vulgaris

Abstract: OATAO is an open access repository that collects the work of some Toulouse researchers and makes it freely available over the web where possible.

Cited by 91 publications

References 34 publications

A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

A co-cultivation process of Nannochloropsis oculata and Tisochrysis lutea induces morpho-physiological and biochemical variations potentially useful for biotechnological purposes

Current and novel approaches to downstream processing of microalgae: A review

The advances and limitations in biodiesel production: feedstocks, oil extraction methods, production, and environmental life cycle assessment

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