Abbreviations:SFE: Supercritical fluid extraction GC-MS: Gas chromatography-mass spectrometryThe essential oil of Marchantia convoluta was obtained by supercritical (carbon dioxide) extraction using methanol as a modifier. Global yields were determined according to the orthogonal design. Compared to M. polymorpha, M. convoluta is quite rare and was thought of negligible by people many years ago. The major identified constituents in M. convoluta were flavonols, triterpenoids, and steroids (Cao et al. 2005;Chen and Xiao, 2005;Chen and Xiao, 2006; Xiao et al. 2005a; Xiao et al. 2005b; Xiao et al. 2006a;Zhu et al. 2005;Zhu et al. 2003). The flavonoids of M. convoluta mainly consist of quercetin, luteolin, apigenin and their O-and C-glycosides (Chen and Xiao, 2005; Xiao et al. 2005a; Xiao et al. 2005b;