Supercritical CO2 Fluid Extraction of Elaeagnus mollis Diels Seed Oil and Its Antioxidant Ability

Abstract

Supercritical fluid carbon dioxide (SF-CO2) was used to extract oil from Elaeagnus mollis Diels (E. mollis Diels) seed and its antioxidant ability was also investigated. The effect of extraction pressure (20–35 MPa), extraction temperature (35–65 C), extraction time (90–180 min) and seed particle size (40–100 mesh) on the oil yield were studied. An orthogonal experiment was conducted to determine the best operating conditions for the maximum extraction oil yield. Based on the optimum conditions, the maximum yield reached 29.35% at 30 MPa, 50 C, 150 min, 80 mesh seed particle size and 40 g/min SF-CO2 flow rate. The E. mollis Diels seed (EDS) oil obtained under optimal SF-CO2 extraction conditions had higher unsaturated fatty acid content (91.89%), higher vitamin E content (96.24 ± 3.01 mg/100 g) and higher total phytosterols content (364.34 ± 4.86 mg/100 g) than that extracted by Soxhlet extraction (SE) and cold pressing (CP) methods. The antioxidant activity of the EDS oil was measured by DPPH and hydroxyl radical scavenging test. EDS oil extracted by different methods exhibited a dose-dependent antioxidant ability, with IC50 values of no significant differences. Based on the results of correlation between bioactive compounds, lupeol and -tocopherol was the most important antioxidant in EDS oil.

Characterization and Biological Activities of Seed Oil Extracted from Berberis dasystachya Maxim. by the Supercritical Carbon Dioxide Extraction Method

“…The yield of BDSO extracted by the SC-CO 2 method ranged from 10.39% to 12.44%, which was higher than that extracted by the PEE method, suggesting that the extraction of BDSO by the SC-CO 2 method is superior to that by the PEE method. This is in agreement with previously reported results [17]. In addition, it is worth noting that BDSO extracted by the PEE method contained traces of organic solvents.…”

“…SC-CO 2 extraction is efficient, safe, and can reduce the use of organic solvents for oil extraction. The SC-CO 2 method could extract BDSO with a superior extraction yield compared to conventional PEE methods, as described previously [17,31]. Thus, we can conclude that SC-CO 2 extraction method is an acceptable and environmentally friendly procedure for oil extraction from B. dasystachya seeds.…”

“…Temperature, pressure, extraction time, and CO 2 flow rate have been optimized to obtain the optimal extraction conditions. Response surface methodology (RSM) has been verified to be a statistical and a mathematical tool for optimizing the reaction parameters, and has also successfully been used to optimize extraction conditions using SC-CO 2 in pharmaceutical research [17,18]. However, studies on extraction of BDSO using the SC-CO 2 method have not been reported.…”

“…Figure 1a-c shows the effects of extraction pressure, extraction temperature, and CO 2 flow rate on the oil yield of BDSO. Extraction pressure is an important extraction parameter that can significantly affect the efficiency of the SC-CO 2 extraction method [17]. Pressure of above 35 MPa can not only lead to high energy consumption, but can also reduce the safety of the equipment.…”

Recent advances of green pretreatment techniques for quality control of natural products

“…selectively [74]. Various kinds of oils [75][76][77][78][79][80] and phenolic compounds [81][82][83][84][85][86] in NPs, which have certain activities, were also could be extracted effectively by CO 2 SFE when the related factors were optimized.…”