An underwater acoustic camera enclosed in a pressure-resistant case was constructed to observe underwater marine animals. This enabled the measurement of the size, shape, and behavior of living marine animals in the detection range up to 240 cm. The transducer array of the acoustic camera was driven by 3.5 MHz ultrasonic signals, and B-mode acoustic images were obtained. Observations were conducted for captive animals in a water tank and for natural animals in a field. The captive animals, including fish, squid and jellyfish, were observed, and a three-dimensional internal structure of animals was reconstructed using multiple acoustical images. The most important contributors of acoustic scattering were the swimbladder and vertebra of bladdered fish, and the liver and reproductive organs of invertebrate animals. In a field experiment, the shape, size, and swimming behavior of wild animals were observed. The possibilities and limitations of the underwater acoustic camera for fishery applications were discussed.