2014
DOI: 10.1021/ed500299j
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“Supermarket Column Chromatography of Leaf Pigments” Revisited: Simple and Ecofriendly Separation of Plant Carotenoids, Chlorophylls, and Flavonoids from Green and Red Leaves

Abstract: A simple and ecofriendly procedure was developed in order to prepare extracts from red and green leaves. This procedure enables the separation of yellow, green, and red band pigments and optimizes the previously reported baking soda “supermarket column”. The same extract also led to a novel and colorful potato starch column, which can efficiently separate β-carotene, xanthophylls, chlorophylls a and b, and flavonoids. Thus, two simple and attractive “supermarket columns” are presented that may be implemented e… Show more

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Cited by 22 publications
(34 citation statements)
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“…Green and purple samples result in the best color separations, probably because yellow and red components are strongly retarded by corn starch. Starch is a polysaccharide with a combination of maltose units, containing many hydrophilic groups . Yellow and red should be the more polar components in the samples.…”
Section: Resultsmentioning
confidence: 99%
“…Green and purple samples result in the best color separations, probably because yellow and red components are strongly retarded by corn starch. Starch is a polysaccharide with a combination of maltose units, containing many hydrophilic groups . Yellow and red should be the more polar components in the samples.…”
Section: Resultsmentioning
confidence: 99%
“…However, there are methods that facilitate the isolation of pigments after extraction and before purification/characterization of chlorophyll. These preparatory methods are thin-layer chromatography or column chromatography, which reduce time and effectively separate the chlorophyll [238,242].…”
Section: Extraction and Purification Methodsmentioning
confidence: 99%
“…Preservice teachers (tertiary education-pedagogical studies) were addressed in nine articles. Some of these articles described examples of the integration of green chemistry experimental work [39,83,[92][93][94][95][96][97], whereas others evaluated activities for integration of green chemistry into experimental work [95,97,98]. The incorporation of green chemistry experiments into the education of pre-service teachers was recognized as a way to promote the development of students' pro-environmental attitudes, values, knowledge, and motivation [39,[95][96][97].…”
Section: What Education Levels Do the Reviewed Papers Focus On?mentioning
confidence: 99%