Superovulatory response following FSH, eCG-FSH and hMG and pregnancy rates following transfer of hatched blastocyst embryos with different diameter and shape in dromedary camel

Ararooti, T.; Niasari-Naslaji, Amir; Asadi-Moghaddam, B.; Razavi, K.; Panahi, Farnaz

doi:10.1016/j.theriogenology.2017.10.017

Cited by 15 publications

(9 citation statements)

References 23 publications

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“…The diameter size of ovulating follicle in this present study is smaller than in crossbred cows was 13.6 ±0.5 mm in ECP protocol and 13.5± 0.7 mm [15], was 13 mm [2]. However this study is similar to [16] that diameter size of ovulating follicle range from ≤6mm to ≥ 9 mm with mean is 13.1±0.26 mm in the GnRH-FSH analogue, 8.2 ±1.16 mm in the hMG, and 4.2±1.07 mm in the eCG-FSH. The diameter of follicle dominant on d2 was similar among primiparous (12.9±0.56 mm than the dominant follicle of multiparous cows that became pregnant (15.6±0.37 mm) [17].…”

Section: A Ovarian Responses Of Cows Treated Using Gnrh Plus Progestsupporting

confidence: 39%

Application of Embryo Transfer Technology to Improve Fertility Using GnRH Plus Progesterone and Estradiol Combination (FTAI) of Local South Pesisir Cow in West Sumatra

Udin

Hendri

Masrizal

2018

Int. J. Adv. Sci. Eng. Inf. Technol.

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The objective of this research was to evaluate the effect of FTAI on embryo production that used GnRH plus progesterone combination to synchronize ovulation of 3 different parity of donor from local pesisir cows in west Sumatra. This study used timed embryo transfer program using GnRH plus progesterone combination in 3 local pesisir cows and 2 cows in time embryo program using estradiol plus progesterone of donor. Ovarian ultrasonography was performed on d 0 (time of AI) and at flushing to determine ovulatory diameter and ovulation. On d 0 at the time CIDR implant + PGF2α injection and on d 7 started ovsynch protocol administrated by injection GnRH and FSH for FTAI without detection estrus. The number and the quality of embryo was evaluated to determine the fertility of pesisir selatan cows. The result showed that the expression of estrus cows tend to high ovulation rate. The diameter size of a follicle was range from 5 to 15 mm with 10.67±2.96 mm in GnRH plus progesterone and 5 to 14 mm with mean was 8.75±2.05 mm in estradiol plus progesterone (P<0.005). The number of follicle ovulation was 27 in treatment A and 18 in treatment B (P<0.05). The number of CL and embryos was higher in treatment A. CL was 19, with 18 embryos and in treatment B was 9 with 6 embryos. The quality of embryo range from 1-4 grade with the transferable embryos was higher in treatment A than treatment B (P<0.05). There was the linear regression between ovular follicle, CL and number of embryos recovery of local pesisir cows. It was concluded that equal numbers of embryos and transferable embryo by using GnRH plus progesterone combination of local pesisir cows.

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Section: A Ovarian Responses Of Cows Treated Using Gnrh Plus Progestsupporting

confidence: 39%

Application of Embryo Transfer Technology to Improve Fertility Using GnRH Plus Progesterone and Estradiol Combination (FTAI) of Local South Pesisir Cow in West Sumatra

Udin

Hendri

Masrizal

2018

Int. J. Adv. Sci. Eng. Inf. Technol.

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“…The effect of the shape of embryo on pregnancy and pregnancy loss rates obtained in the present study confirms our previous results on cooled dromedary embryos (Abd‐Elfattah et al., 2020). On the other hand, there was a non‐significant difference in the pregnancy rate at Day 33 of gestation among different shapes of embryos (round, oval and collapsed) in a study in which a small number of embryos ( n = 139) was transferred (Ararooti et al., 2018). In our previous study using fresh dromedary embryos, shape of the embryo had a non‐significant effect on the pregnancy losses between Days 19 and 60 (Karen & Mansour, 2020).…”

Section: Discussionmentioning

confidence: 97%

“…On the contrary, Ararooti et al. (2018) reported that the pregnancy rate at Day 32 was significantly greater if a medium‐sized camel embryo was transferred into recipients compared with small‐ and large‐sized embryo. In the latter study, the effect of embryo size and shape was evaluated separately by using the chi‐squared test, not by the logistic regression model used in the present study.…”

Section: Discussionmentioning

confidence: 98%

Factors affecting outcomes of embryo transfer in dromedary camels: A retrospective study

Karen

Abd-Elfattah

Nasef

et al. 2022

Reprod Domestic Animals

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The present study aimed to use a comprehensive approach for evaluating 12 factors related to embryo quality (shape, size and transparency), donors (age, ET type, number of recovered embryos and day of uterine flushing), recipients (age), males (age and individual variations) and environment (season and year) which could affect the outcome of ET in terms of pregnancy (PR) and pregnancy losses in dromedary camels. During three breeding seasons, 116 donor females were mated repeatedly at 12‐ to 14‐day intervals by fertile male camels (n = 33) without stimulation of the ovaries (WSPO). Superovulation (SPO ET) regimen was applied for each donor female twice or thrice per season. In the occasions of applying superovulation regimen, donor females having an ovulatory follicle were mated instead of GnRH administration and superovulation regimen was applied 4 days post‐mating (MIX ET). The uteri of all donor females were flushed at Day 8 or 9 post‐mating, and a total of 2,095 embryos were recovered and transferred individually to 924 recipient females. Pregnancy diagnoses were conducted at Day 10 after ET (Days 18–19 of gestation) by using progesterone assay and by transrectal ultrasonography (TRU) at Days 30 and 60 of gestation. By using logistic regression analysis, transparency of embryos and age of recipient females had significant effects on PR at Days 18–19 (p < .01), 30 (p < .01) and 60 (p < .01; p < .05, respectively) of gestation. The shape of embryos had significant effects on the PR at Days 30 (p < .05) and 60 (p < .01) of gestation. Type of ET and the breeding season (year) had significant (p < .05) effects on the PR at Day 30, while day of flushing had the same effect on PR at Day 60. Regarding the pregnancy losses, transparency and shape of the embryo, type of ET, breeding season had significant (p < .05) effect on the late embryonic mortalities (LEM) and shape and season of year had significant (p < .01 and p < .05, respectively) effect on LEM/early foetal mortalities (EFM). Regarding male individual factor, there was a tendency for a significant (p = .055) effect of male camels on the PR at Days 18–19 and rate of LEM. In conclusion, transferring a spherical, transparent or a large‐sized embryo (>750 µm) into recipient females ageing between 8 and 11 y could greatly improve the PR from Days 18 to 60 of gestation. Also, embryo recovered from donor females with Mix ET type or embryos sired by certain male camel or at Day 8 post‐mating of the donor could improve the 2‐month PR. In addition, transferring a transparent or spherical‐shaped embryo or embryos recovered from donor females with SPO or Mix ET could reduce the pregnancy losses during the first 2 months of pregnancy.

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“…Hompes et al [37] showed that hMG resulted in a lower incidence of hyper-response. Furthermore, Coomarasamy et al [38] presented a pooled 4% increase in live birth rate after the use of by hMG relative to rFSH, whereas Ararooti et al [39] reported that the FSH protocol provided a better superovulatory response and a higher number of embryos. Until now, there has been no systematic study of the similarities and differences in DNA methylation alterations associated with superovulation using FSH/ hMG + hCG.…”

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confidence: 99%

Single-cell DNA methylation sequencing reveals epigenetic alterations in mouse oocytes superovulated with different dosages of gonadotropins

et al. 2020

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Background: Epigenetic abnormalities caused by superovulation have recently attracted increasing attention. Superovulation with exogenous hormones may prevent oocytes from establishing an appropriate epigenetic state, and this effect may extend to the methylation programming in preimplantation embryos, as de novo DNA methylation is a function of developmental stage of follicles and oocyte size. Follicle-stimulating hormone (FSH) and human menopausal gonadotropin (hMG) are common gonadotropins used for superovulation, and appropriate concentrations of these gonadotropins might be necessary. However, no systematic study on the effects of DNA methylation alterations in oocytes associated with superovulation with different dosages of FSH/hMG at the singlecell level has yet been reported. In the current study, different dosages of FSH/hMG combined with human chorionic gonadotropin (hCG) were used in female mice to generate experimental groups, while naturally matured oocytes and oocytes superovulated with only hCG were respectively used as controls. Single-cell level DNA methylation sequencing was carried out on all these matured oocytes.Results: In this study, we revealed that the genome-wide methylation pattern and CG methylation level of the maternal imprinting control regions of all mature oocytes were globally conserved and stable. However, methylation alterations associated with superovulation were found at a specific set of loci, and the differentially methylated regions (DMRs) mainly occurred in regions other than promoters. Furthermore, some of the annotated genes in the DMRs were involved in biological processes such as glucose metabolism, nervous system development, cell cycle, cell proliferation, and embryo implantation and were altered in all dosages of FSH/hMG group (for example, Gfod2 and SYF2). Other genes were impaired only after high gonadotropin dosages (for instance, Sox17 and Phactr4).

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Superovulatory response following FSH, eCG-FSH and hMG and pregnancy rates following transfer of hatched blastocyst embryos with different diameter and shape in dromedary camel

Cited by 15 publications

References 23 publications

Application of Embryo Transfer Technology to Improve Fertility Using GnRH Plus Progesterone and Estradiol Combination (FTAI) of Local South Pesisir Cow in West Sumatra

Application of Embryo Transfer Technology to Improve Fertility Using GnRH Plus Progesterone and Estradiol Combination (FTAI) of Local South Pesisir Cow in West Sumatra

Factors affecting outcomes of embryo transfer in dromedary camels: A retrospective study

Single-cell DNA methylation sequencing reveals epigenetic alterations in mouse oocytes superovulated with different dosages of gonadotropins

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