Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Hiraizumi, S.; Nishinomiya, H.; Oikawa, Toshinori; Sakagami, Nobutada; Sano, F.; Nishino, Osamu; Kurahara, Takami; Nishimoto, Namiko; Ishiyama, O.; Hasegawa, Yoshihisa; Hashiyada, Y.

doi:10.1016/j.theriogenology.2014.09.012

Cited by 23 publications

(20 citation statements)

References 35 publications

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“…The traditional superstimulatory protocol uses saline as a diluent, and due to the short half-life of the FSH [39,40], twice-daily IM injections are required for in vivo [36] or in vitro [19,37] embryo production. On the basis of the rapid clearance of FSH (w5 hours) [39], regardless of the dose administered [55], an alternative could be to modify hormone absorption. Modifications examined have included changing the route administration (subcutaneous injection, [55][56][57]) or mixing the pFSH with polymers, such as polyvinylpyrrolidone [58], aluminum hydroxide gel [59] or HA [42] to reduce the rate of absorption.…”

Section: Discussionmentioning

confidence: 99%

“…On the basis of the rapid clearance of FSH (w5 hours) [39], regardless of the dose administered [55], an alternative could be to modify hormone absorption. Modifications examined have included changing the route administration (subcutaneous injection, [55][56][57]) or mixing the pFSH with polymers, such as polyvinylpyrrolidone [58], aluminum hydroxide gel [59] or HA [42] to reduce the rate of absorption. Tríbulo et al [42] reported satisfactory superovulatory results after treating Table 1 Summary of cumulus-oocyte complex yield and in vitro embryo production (mean AE standard error or percentage) in nonlactating Holstein cows that were not treated (control), treated with 200 mg of porcine FSH (pFSH; F200) in twice-daily injections or 200 or 300 mg of pFSH as a single injection in 0.5% hyaluronan (F200HA and F300HA).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Vieira¹,

Rodrigues

Netto

et al. 2016

Theriogenology

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Plasma FSH profiles, in vitro embryo production (IVP) after ovum pickup (OPU), and establishment of pregnancy with IVP embryos were compared in untreated Holstein oocyte donors and those superstimulated with multiple injections or a single intramuscular (IM) injection of porcine FSH (pFSH) in hyaluronan (HA). Plasma FSH profiles were determined in 23 heifers randomly allocated to one of four groups. Controls received no treatment, whereas the F200 group received 200 mg of pFSH in four doses, 12 hours apart. The F200HA and F300HA groups received 200- or 300-mg pFSH in 5 mL or 7.5 mL, respectively of a 0.5% HA solution by a single IM injection. Plasma FSH levels were determined before the first pFSH treatment and every 6 hours over 96 hours. All data were analyzed by orthogonal contrasts. Circulating FSH area under curve (AUC) in pFSH-treated animals was greater than that in the control group (P = 0.02). Although the AUC did not differ among FSH-treated groups (P = 0.56), the total period with elevated plasma FSH was greater in the F200 group than in the HA groups (P < 0.0001). However, the F300HA group had a greater AUC than the F200HA group (P = 0.006), with a similar total period with elevated plasma FSH (P = 0.17). The IVP was performed in 90 nonlactating Holstein cows randomly allocated to one of the four treatment groups as in the first experiment. A greater proportion of medium-sized (6-10 mm) follicles was observed in cows receiving pFSH, regardless of the treatment group (P < 0.0001). Also, numbers of follicles (P = 0.01), cumulus-oocyte complexes (COCs) retrieved (P = 0.01) and matured (P = 0.02), cleavage rates (P = 0.002), and blastocysts produced per OPU session (P = 0.06) were greater in cows receiving pFSH, regardless of the treatment group. Cows in the F200HA group had a greater recovery rate (P = 0.009), number of COCs cultured (P = 0.04), and blastocysts produced per OPU session (P = 0.06) than cows in the F300HA group. Similar pregnancy rates were observed 50 to 60 days after transferring IVP embryos from donors in the different treatment groups (P > 0.05). In conclusion, a single IM injection of pFSH combined in 0.5% HA resulted in similar plasma FSH profiles as twice-daily pFSH treatments. Treatment of nonlactating donors with pFSH, with or without HA, resulted in increased IVP over untreated controls. A single dose of 200 mg of pFSH in 0.5% HA resulted in greater IVP than 300-mg pFSH in HA. Finally, pregnancy rates with IVP embryos were similar, regardless donor treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Vieira¹,

Rodrigues

Netto

et al. 2016

Theriogenology

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“…An important factor determining the rate of the absorption process is the body condition score of the animal (BCS), thus the amount of fat tissue at the place of deposition of the drug (6,18,43). It was shown that the reaction to a single dose of the preparation in the neck area was weaker than after its administration in the shoulder region in cows in insufficient condition.…”

Section: Conventional Methods Of Inducing Superovulation In Cattlementioning

confidence: 99%

“…However, while the animal was in good condition or overweight, the response was satisfactory regardless of the place of administration. Since subcutaneous injection behind the shoulder generated a comparable response regardless of the BCS value, a hypothesis was made that the amount of fat tissue and the place of deposition could be critical points in the process of slowing down the absorption of pFSH (18,43). For this reason, deposition of the drug in animals with lower BCS values or genetically predisposed to lower fat cover is suggested in the shoulder region.…”

Section: Conventional Methods Of Inducing Superovulation In Cattlementioning

confidence: 99%

Superovulation in cattle – searching for the optimal dose, alternative routes of administration and a simplified FSH application program

Kulus¹,

Wieczorkiewicz²,

Kulus³

et al. 2019

Medycyna Weterynaryjna

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The aim of this article is to set out certain possibilities related to the simplification of the superovulation protocols (SOV) in cattle. The traditional protocols, which have been in use for decades, include as standard several injections of FSH preparations. Due to the widespread increase in the importance of the animals’ welfare, alternative options for its administration are now being sought. Appropriate selection of gonadotropin as well as the dose and ratio of hormones makes it possible to limit undesirable effects such as prolonged ovarian stimulation. Adjuvants such as polyvinylpyrrolidone, polyethylene glycol, hyaluronic acid or aluminium hydroxide enable the extension of the absorption time of FSH preparations, thus reducing the amount of FSH injections. A single deposition of hormones dissolved in aqueous solutions is possible when it is administered via alternative routes, such as subcutaneous or epidural. Reduction of the amount of FSH injections may directly translate into a decrease of the stress level in animals treated by the SOV protocols and also to the improvement of the efficiency of embryo production. A significant risk factor remains the high variability of superovulation. Its effectiveness depends on a number of generally well known individual and environmental factors that should also be considered when joining SOV.

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“…DEMOUSTIER et al, 1988), regardless of dose of administered (HIRAIZUMI et al, 2015), an alternative could be to modify hormone absorption. Modifications examined have included changing the route administration (subcutaneous injection - BO et al, 1994;KELLY et al, 1997;HIRAIZUMI et al, 2015) or mixing the pFSH with polymers, such as polyvinylpyrrolidone (CHASOMBAT et al, 2013), aluminum hydroxide gel (HASHIMOTO et al, 2007) or hyaluronan (TRÍBULO, A. et al, 2012) in order to reduce the rate of absorption. Tríbulo et al (2012), reported satisfactory superovulatory results after treating donors with a single dose of pFSH combined with 2% HA.…”

Section: Experiments 2: Ovum Pick-up In Vitro Embryo Production and Ementioning

confidence: 99%

<b>Suplementação exógena com gonadotrofinas para aumentar a produção <i>in vitro</i> de embriões em doadoras Holandesas</b>

Vieira¹

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Superovulatory response in Japanese Black cows receiving a single subcutaneous porcine follicle–stimulating hormone treatment or six intramuscular treatments over three days

Cited by 23 publications

References 35 publications

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

Superovulation in cattle – searching for the optimal dose, alternative routes of administration and a simplified FSH application program

<b>Suplementação exógena com gonadotrofinas para aumentar a produção <i>in vitro</i> de embriões em doadoras Holandesas</b>

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