Superoxide Anion-Mediated Afterglow Mechanism-Based Water-Soluble Zwitterion Dye Achieving Renal-Failure Mice Detection

Li, Zhe; Xu, Li; Li, Jin-Yu; Lei, Lingling; Liang, Ping-Zhao; Wu, Qian; Yang, Feiyu; Ren, Tian-Bing; Yin, Xia; Yuan, Lin; Zhang, Xiao-Bing

doi:10.1021/jacs.3c08579

Cited by 20 publications

(13 citation statements)

References 46 publications

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“…After 2 h, the fluorescence in the AS model mice was decreased to a level that was only slightly higher than that of the healthy mice (Figure b,d,e). These results suggest that BSJD can be rapidly metabolized in mice, , which benefits to avoid long-term accumulation, protect organ function, improve experimental flexibility, and reduce the risk of adverse reactions, thus holding great promise in early diagnosis and in vivo monitoring of AS.…”

Section: Resultsmentioning

confidence: 99%

Multiparameter Assessment of Foam Cell Formation Progression Using a Dual-Color Switchable Fluorescence Probe

Li,

Han,

Wang

et al. 2024

Anal. Chem.

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The assessment of atherosclerosis (AS) progression has emerged as a prominent area of research. Monitoring various pathological features of foam cell (FC) formation is imperative to comprehensively assess AS progression. Herein, a simple benzospiropyran-julolidine-based probe, BSJD, with switchable dual-color imaging ability was developed. This probe can dynamically and reversibly adjust its molecular structure and fluorescent properties in different polar and pH environments. Such a polarity and pH dual-responsive characteristic makes it superior to single-responsive probes in dual-color imaging of lipid droplets (LDs) and lysosomes as well as monitoring their interaction. By simultaneously tracking various pathological features, including LD accumulation and size changes, lysosome dysfunction, and dynamically regulated lipophagy, more comprehensive information can be obtained for multiparameter assessment of FC formation progression. Using BSJD, not only the activation of lipophagy in the early stages and inhibition in the later phases during FC formation are clearly observed but also the important roles of lipophagy in regulating lipid metabolism and alleviating FC formation are demonstrated. Furthermore, BSJD is demonstrated to be capable of rapidly imaging FC plaque sites in AS mice with fast pharmacokinetics. Altogether, BSJD holds great promise as a dual-color organelle-imaging tool for investigating disease-related LD and lysosome changes and their interactions.

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Section: Resultsmentioning

confidence: 99%

Multiparameter Assessment of Foam Cell Formation Progression Using a Dual-Color Switchable Fluorescence Probe

Li,

Han,

Wang

et al. 2024

Anal. Chem.

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“…Indocyanine green (ICG), organic luminogens hold significant promise for bioimaging applications compared to inorganic counterparts . Common approaches to design organic NIR-II dyes include conjugation extension and donor–acceptor (D-A) engineering .…”

mentioning

confidence: 99%

“…Indocyanine green (ICG), organic luminogens hold significant promise for bioimaging applications compared to inorganic counterparts. 12 Common approaches to design organic NIR-II dyes include conjugation extension 13 and donor−acceptor (D-A) engineering. 14 However, extended conjugation and D−A structures often lead to strong intermolecular π−π and D−A interactions, resulting in aggregation-caused quenching (ACQ).…”

mentioning

confidence: 99%

Ultrabright NIR-II Nanoprobe for Image-Guided Accurate Resection of Tiny Metastatic Lesions

Xu,

Yuan,

Shi

et al. 2024

Nano Lett.

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Fluorescence imaging is a vital way to delineate the tumor boundaries. Here, we achieve a NIR-II aggregation-induced emission luminogen (AIEgen) with a fluorescence quantum yield (QY) of 12.6% in water through straightforward alkyl side chain modification. After loading of NIR-II AIEgen into polystyrene (PS) nanospheres, the thermal deactivation pathway is extremely limited, thereby concentrating absorption excitation on fluorescence emission. The fluorescence intensity is further enhanced by 5.4 times, the QY increases to 21.1%, and the NIR-II imaging signal is accordingly enhanced by 8.7 times, surpassing conventional DSPE-PEG carriers. The NIR-II@PS nanoprobe showcases superior resolution and tissue penetration depth compared to indocyanine green (ICG) and short-range near-infrared AIEgens. In vivo investigations underscore its tumor-to-normal tissue ratio (3.9) at 24 h post intravenous injection, enabling complete resection of ≤1 mm metastases under NIR-II bioimaging guidance. Additionally, the PS carrier-nanoparticles exhibit low toxicity in vivo, laying a promising foundation for the future design of medical nanomaterials.

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“…Given the high reactivity and short lifespan of O 2 ·– and ONOO – , conventional histological and biochemical analysis techniques lack the ability to assess their dynamic changes within cells or in vivo in real-time. Fluorescence imaging offers a crucial tool for the noninvasive investigation of biological processes in cells and transparent organisms due to its superior spatiotemporal resolution, sensitivity, and ability to detect targets in situ and/or in real-time within complex biosystems − While numerous fluorescent probes have been developed for the specific detection of O 2 ·– − or ONOO – , − few have been able to simultaneously visualize both species in vitro and in vivo . Although it is possible to use a single fluorescent probe for O 2 ·– and another for ONOO – simultaneously, data interpretation becomes challenging due to their different uptake, distribution, and metabolism profiles.…”

mentioning

confidence: 99%

A Sequentially Activated Probe for Imaging of Superoxide Anion and Peroxynitrite in PC12 Cells under Oxidative Stress

Li,

Cao,

et al. 2024

Anal. Chem.

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Superoxide anion (O 2 •− ) and peroxynitrite (ONOO − ), two important oxidants under oxidative stress, coexist in complex cell and organism systems, playing crucial roles in various physiological and pathological processes, particularly in neurodegenerative diseases. Despite the absence of robust molecular tools capable of simultaneously visualizing O 2•− and ONOO − in biosystems, the relationship between these two species remains understudied. Herein, we present sequentially activated fluorescent probe, DHX-SP, which exhibits exceptional selectivity and sensitivity toward O 2•− and ONOO − . This probe enables precise imaging of these species in living PC12 cells under oxidative stress conditions using distinct fluorescence signal combinations. Furthermore, the probe DHX-SP has the ability to visualize changes in O 2•− and ONOO − levels during ferroptosis of PC12 cells and in the Parkinson's disease model. These findings establish a connection between the crosstalk of the phosphorus group of O 2•− and ONOO − in PC12 cells under oxidative stress.

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Superoxide Anion-Mediated Afterglow Mechanism-Based Water-Soluble Zwitterion Dye Achieving Renal-Failure Mice Detection

Cited by 20 publications

References 46 publications

Multiparameter Assessment of Foam Cell Formation Progression Using a Dual-Color Switchable Fluorescence Probe

Multiparameter Assessment of Foam Cell Formation Progression Using a Dual-Color Switchable Fluorescence Probe

Ultrabright NIR-II Nanoprobe for Image-Guided Accurate Resection of Tiny Metastatic Lesions

A Sequentially Activated Probe for Imaging of Superoxide Anion and Peroxynitrite in PC12 Cells under Oxidative Stress

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