Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium

Pejić, Snežana; Todorović, Ana; Stojiljković, Vesna; Cvetković, Dragana; Lucic, Nenad; Radojičić, R.; Saičić, Zorica S.; Pajović, Snežana B.

doi:10.1590/s0001-37652008000300011

Cited by 23 publications

(13 citation statements)

References 30 publications

(19 reference statements)

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“…Similar results were obtained in endometrial cancer tissue of both Finnish and Japanese women [14]. The decrease in enzyme activity appeared to be related to the decreased levels of SOD protein in the examined tissues [25]. Also, it may be due to an increased endogenous production of ROS as evidenced by increased lipid hydroperoxides.…”

Section: Discussionsupporting

confidence: 68%

Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma

Pejić

Todorović

Stojiljković

et al. 2009

Reprod Biol Endocrinol

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BackgroundOxidative stress and impaired antioxidant system have been proposed as a potential factors involved in the pathophysiology of diverse disease states, including carcinogenesis. In this study, we explored the lipid peroxidation levels and antioxidant enzyme activities in women diagnosed with different forms of gynecological diseases in order to evaluate the antioxidant status in endometrium of such patients.MethodsEndometrial tissues of gynecological patients with different diagnoses were collected and subjected to assays for superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and lipid hydroperoxides.ResultsSuperoxide dismutase activity was significantly decreased (50% in average) in hyperplastic and adenocarcinoma patients. Activities of both glutathione peroxidase and glutathione reductase were increased 60% and 100% on average, in hyperplastic patients, while in adenocarcinoma patients only glutathione reductase activity was elevated 100%. Catalase activity was significantly decreased in adenocarcinoma patients (47%). Lipid hydroperoxides level was negatively correlated to superoxide dismutase and catalase activities, and positively correlated to glutathione peroxidase and glutathione reductase activities.ConclusionsThis study provided the first comparison of antioxidant status and lipid peroxidation in endometrial tissues of patients with polyps, myoma, hyperplasia and adenocarcinoma. The results showed that patients with premalignant (hyperplastic) and malignant (adenocarcinoma) lesions had enhanced lipid peroxidation and altered uterine antioxidant enzyme activities than patients with benign uterine diseases, polyps and myoma, although the extent of disturbance varied with the diagnosis. Further investigation is needed to clarify the mechanisms responsible for the observed alterations and whether lipid hydroperoxide levels and antioxidant enzyme activities in uterus of gynecological patients might be used as additional parameter in clinical evaluation of gynecological disorders.

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Section: Discussionsupporting

confidence: 68%

Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma

Pejić

Todorović

Stojiljković

et al. 2009

Reprod Biol Endocrinol

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“…The decrease in SOD activity appeared to be associated with the decrease in the levels of SOD protein of the examined tissues [32]. In addition, this may be due to an increasing endogenous production of ROS as indicated by the increase in lipid hydroperoxides.…”

Section: Discussionmentioning

confidence: 86%

Effect of Daily Chewing Soft Buds and Leaves of Catha edulis (Khat) on the Antioxidant Defense System and Oxidative Stress Markers in Blood

et al. 2014

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Catha edulis (Khat) is one of the major economic, social and health problems in Yemen. This paper aimed to study the effect of Khat on the oxidative status of Khat chewers by measuring the levels of enzymatic and non-enzymatic antioxidant as well as lipid peroxidation. The results exhibited significant reduction in erythrocytes superoxide dismutase (SOD, EC: 1.15.1.1), and catalase (CAT, EC: 1.11.1.6) in Khat chewers, in addition to elevation of serum glutathione-S-transferase (GST, EC: 2.5.1.18). Furthermore, non-enzymatic antioxidants glutathione (GSH) and vitamin C were significantly reduced ( p < 0.001; p < 0.015), whereas malondialdehyde (MDA) was significantly elevated ( p < 0.001). The depletion of GSH and vitamin C along with MDA elevation in Khat chewers compared with control reflects the obvious oxidative status, a result of enormous reactive oxygen species (ROS) formation, leading to membrane damage. ROS possibly induced by active components of Khat or by pesticides added to the Khat tree. In addition, the reduction of SOD and CAT is indicative to cellular proteins damage which occurred by ROS. As well, the elevation of GST may due to a leakage of cellular GST to blood stream; this implies that GST active site was not affected. This study concludes that daily chewing Khat for long period certainly induce ROS production, leading to oxidative toxicity. Both enzymatic and non-enzymatic antioxidants are involved in the protection against this toxicity. People who habitually chew Khat for long term will be susceptible to the oxidative toxicity; therefore, they recommended giving up of Khat chewing.

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“…This was a common finding in all patients and was not dependent on the chemotherapeutic agent used. A 10% -20% decrease in TAC is a common characteristic in patients suffering from cancer and was observed in benign and malignant neoplasias [39]. Emotional and biological stress and cytotoxic procedures may also contribute to that decrease.…”

Section: Discussionmentioning

confidence: 99%

Cortisol levels and serum antioxidant status following chemotherapy

Limberaki¹,

Eleftheriou²,

Gasparis³

et al. 2011

Health

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Chemotherapy results in increased free radical formation and depletion of tissue antioxidants. Moreover, patients receiving chemotherapy are under emotional stress, which is also accompanied by low antioxidant levels. In the present study, we measured cortisol, the main stress hormone, and total antioxidant capacity (TAC) in serum of 51 cancer patients during chemotherapy. Antioxidant activity was estimated by measuring the influence of serum in oxidation of ABTS (2,2΄-azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid to ABTS+ by methmyoglobin (Antioxidant kit of Cayman). Serum cortisol was measured using an ELISA colorimetric assay. Serum TAC was significantly decreased (75% decrease compared to normal levels, p = 0.001) in all patients during chemotherapy, while blood cortisol concentration was increased by 10%, (p = 0.044). Lower antioxidant levels and higher cortisol concentration were detected in patients receiving chemotherapeutic drugs daily, compared to the ones receiving chemotherapy once a week. A difference between sexes was observed with male patients presenting lower antioxidant status and higher cortisol levels than females. A significant and persistent decrease in antioxidant capacity accompanied by increased cortisol concentration was observed in all patients during chemotherapy. This fact, which is probably generated by biological and emotional stress, increases the probability of harmful side effects and organism weakening and needs to be considered during patients' treatment.

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Superoxide dismutase and lipid hydroperoxides in blood and endometrial tissue of patients with benign, hyperplastic and malignant endometrium

Cited by 23 publications

References 30 publications

Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma

Antioxidant enzymes and lipid peroxidation in endometrium of patients with polyps, myoma, hyperplasia and adenocarcinoma

Effect of Daily Chewing Soft Buds and Leaves of Catha edulis (Khat) on the Antioxidant Defense System and Oxidative Stress Markers in Blood

Cortisol levels and serum antioxidant status following chemotherapy

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