Superoxide dismutase as modulator of immune function in American white shrimp (Litopenaeus vannamei)

Campa‐Córdova, Ángel I.; Hernández-Saavedra, Norma Y.; Ascencio, Felipe

doi:10.1016/s1532-0456(02)00125-4

Cited by 96 publications

(65 citation statements)

References 38 publications

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“…Peak expression was observed 6 h after treatment, and changes in the enzymatic activity of SOD were generally consistent with changes in gene expression. Previous studies have also demonstrated that SOD activity increases in hemocytes and muscle cells of L. vannamei treated with an immunopotentiator, and the survival rate increased following bacterial infection, suggesting that SOD regulates the immune response in shrimps (Campa-Cordova et al, 2002). In crustaceans, SOD and phenoloxidase are two enzymes with roles in the immune system, which may be used as indicator enzymes to detect immune function (Amparyup et al, 2013;Liu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Imidazole derivative KK-42 is a well-known regulator of insect growth. KK-42 pretreatment has been shown to promote the survival of Macrobrachium nipponense infected with Aeromonas hydrophila, possibly via activation of superoxide dismutase (SOD). In this study, the cytMnSOD gene was cloned from the hepatopancreas of M. nipponense using the rapid amplification of cDNA ends technique. The full-length cDNA of cytMnSOD was 1233 bp long, and the open reading frame was 858 bp long, encoding a 286-aa protein with a 60-aa leader sequence. The calculated molecular mass of the translated cytMnSOD protein was 31.33 kDa, with an estimated isoelectric point of 5.62. cytMnSOD contained two N-glycosylation sites, four conserved amino acids responsible for binding manganese, and a manganese SOD domain (DVWEHAYY). Real-time RT-PCR analysis showed that cytMnSOD was expressed in all tissues examined with the highest expression observed in the hepatopancreas. Levels of the cytMnSOD transcript in the hepatopancreas were highest in stage C of the molting cycle. Real-time PCR analysis revealed that cytMnSOD expression increased significantly 3, 6, and 12 h after KK-42 treatment, with simultaneous increases in SOD activity from 6 to 12 h. Our results demonstrate that cytMnSOD expression and SOD activity may be induced by KK-42, which may represent one of the molecular mechanisms through which KK-42 promotes increased survival of prawns infected with A. hydrophila.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Wang

et al. 2016

Genet. Mol. Res.

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“…Superoxide dismutase is the first enzyme to respond against oxygen radicals (McCord and Fridovich 1969) and is the one that offers the greatest response to oxidative stress (Winston and Di Giulio 1991). Campa-Córdova et al (2002) reported haemocytes and muscle showed similar increased levels of superoxide dismutase activity after immersed in aerated b-glucan and sulfated polysaccharide solutions for 6 h and demonstrated the immunomodulatory action of superoxide dismutase and its possible use as an indicator of immune responses in L. vannamei. Catalase is a major primary immune defense component that works primarily to catalyze the decomposition of H 2 O 2 to H 2 O. Lysozyme and alkaline phosphatase are symbol enzymes of lysomase that can damage foreign substance and accelerate hydrolysis and transphosphorylation (Harada 1991;Jolles and Jolles 1984;Yano 1996).…”

Section: Discussionmentioning

confidence: 99%

Effect of long-term administration of dietary β-1,3-glucan on growth, physiological, and immune responses in Litopenaeus vannamei (Boone, 1931)

et al. 2011

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b-1,3-Glucan at different dietary doses was administered to enhance the growth, immunity, and survival against nitrite stress in Pacific white shrimp, Litopenaeus vannamei. Four different diets supplemented with 0, 250, 500, or 1,000 mg of b-1,3-glucan kg -1 diets were fed to L. vannamei. Growth performance (weight gain and survival rate), physiological conditions (blood total protein, glucose, lactate, triacylglycerols, cholesterol levels) and immunological responses (superoxide dismutase, catalase, lysozyme, acid phosphatase, and alkaline phosphatase activities) of shrimp were recorded after 84-day feeding and 120 h after exposed to nitrite-N. After 84-day feeding, 250 mg kg -1 b-1,3-glucan diet resulted in better weight gain (P \ 0.05). Before the nitrite stress, blood lactate, triacylglycerols, and cholesterol level in shrimp fed with 250 mg kg -1 b-1,3-glucan diet were significantly higher than those observed in shrimp fed with other diets (P \ 0.05). Higher activities of catalase, lysozyme, and alkaline phosphatase were observed in shrimp fed with 500 or 1,000 mg kg -1 b-1,3-glucan diet as compared to those obtained in shrimp fed with other diets (P \ 0.05). After 120-h nitrite stress, blood protein, lactate, superoxide dismutase, catalase, and alkaline phosphatase activities in shrimp fed with 500 or 1,000 mg kg -1 b-1,3-glucan were significantly higher than those observed in shrimp fed with other diets (P \ 0.05). Glucose and triacylglycerol levels of shrimp fed with 500 or 1,000 mg kg -1 b-1,3-glucan were significantly lower than those observed in other diets (P \ 0.05). In shrimp fed with 500 and 1,000 mg kg -1 b-1,3-glucan and 120-h after nitrite stress, the mortality was significantly lower than that observed in shrimp of control.Together, in this 84-day feeding trial, 250 mg kg -1 b-1,3-glucan improved growth, whereas 500 mg kg -1 b-1,3-glucan preferentially improved nitrite resistance, probably through accelerating energy metabolism and activating immune system.

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“…The major function of SOD is to scavenge superoxide anion. SOD has been known as the important immune factor in aquatic animal (Campa-Córdova et al 2002;Wang and Chen 2006). In C. farreri, the studies also indicate that SOD activity can reflect the immunological state .…”

Section: Introductionmentioning

confidence: 89%

“…Moreover, many humoral factors also take part in immune defense, such as SOD has been reported to correlate well with immune competence of crustacean. As a free radical elimination enzyme, SOD is essential to minimize the oxidative damage to host cells in the immune defense Campa-Córdova et al 2002). The major function of SOD is to scavenge superoxide anion.…”

Section: Introductionmentioning

confidence: 99%

Effect of hypoxia on immunological, physiological response, and hepatopancreatic metabolism of juvenile Chinese mitten crab Eriocheir sinensis

et al. 2010

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The juvenile Chinese mitten crabs Eriocheir sinensis (1.40 ± 0.43 g) were cultured in water of 5.34 ± 0.43 (hypoxia, treatment group) and 21.02 ± 0.06 kPa (high dissolved oxygen (DO), control group) DO for 24 h, respectively. The total hemocyte counts (THC), the hyaline hemocyte counts were measured at 0 and 24 h in the treatment group, and superoxide dismutase (SOD) activity, concentrations of lactic acid and hemocyanin (Hc) in hemolymph and the metabolism of hepatopancreas (concentrations of glucose, total cholesterol, uric acid, total protein, urea, triglyceride in hepatopancreas) were assayed at 0, 2, 8, and 24 h in both treatment and control groups. The hyaline hemocyte counts and THC decreased significantly by 66 and 49% after exposure to hypoxic water for 24 h, respectively. SOD activity, Hc and lactic acid content were significantly affected in treatment group, and there were significant differences between treatment and control groups. In the treatment group, the concentration of glucose, cholesterol and uric acid in hepatopancreas had a significant variation for 24 h. The concentration of total protein, urea and triglyceride between treatment and control groups was not significantly different after 0, 2, 8, 24 h. However, there were significant differences between treatment and control groups in terms of total protein, glucose, and uric acid concentration. Taken together, the effect of hypoxia is comprehensive in juvenile Chinese mitten crab Eriocheir sinensis.

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Superoxide dismutase as modulator of immune function in American white shrimp (Litopenaeus vannamei)

Cited by 96 publications

References 38 publications

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Effect of long-term administration of dietary β-1,3-glucan on growth, physiological, and immune responses in Litopenaeus vannamei (Boone, 1931)

Effect of hypoxia on immunological, physiological response, and hepatopancreatic metabolism of juvenile Chinese mitten crab Eriocheir sinensis

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