Superoxide dismutase biosensors working in non-aqueous solvent

Abstract

Enzymatic electrodes based on Superoxide dismutase enzyme were developed. Using the Superoxide dismutase enzyme sensor assembled according to the classical model, poor results were obtained. Results were improved by adopting a new way of assembling the biosensor using a cellulose triacetate layer in which the SOD enzyme is entrapped and sandwiched between two gas-permeable membranes, or using a kappa-carrageenan gel layer entrapping the enzyme, sandwiched between an external gas permeable membrane and an internal cellulose acetate membrane, coupled in each case to the oxygen amperometric transducer. Results obtained by applying the newly developed biosensor to assaying hydrophobic compounds showing radical scavenging properties, operating in dimethylsulfoxide, were also satisfactory. © Springer-Verlag 2001

Carboxymethylcellulose–gelatin–superoxidase dismutase electrode for amperometric superoxide radical sensing

“…Reusability is one major advantage of this CMC-G-SOD biosensor. It is one of the longest-lasting biosensors among those yet developed with the advantage of high biosensor response at low enzyme concentrations [25,26,28,42,43]. Immobilization of SOD on to G-CMC provides a biocompatible microenvironment around the enzyme and stabilizes enzyme activity very efficiently.…”

Organic phase enzyme electrodes

“…The enzyme was physically entrapped, using a cellulose triacetate layer and sandwiched between two gas-permeable membranes, or using a kappa-Carrageenan gel layer entrapping the enzyme, sandwiched between an external gas permeable membrane and an internal cellulose acetate membrane, coupled in each case to the oxygen amperometric transducer. This biosensor was applied for the determination of hydrophobic compounds showing radical scavenging properties operating in dimethylsulfoxide with satisfactory results (Campanella et al, 2001d). More recently, it has been reported the measurement of the antioxidant capacity of integrator-phytotherapeutic products using the second of the former immobilized systems.…”

Electrochemical Biosensors for the Determination of the Antioxidant Capacity in Foods and Beverages Based on Reactive Oxygen Species

“…This latter point led the same authors to the development of a biosensor capable of operating in organic solvents, in which these compounds are more soluble. The developed O 2 -biosensor, successfully used in a DMSO solution, was based on SOD entrapped in a cellulose triacetate layer (sandwiched between two gas-permeable membranes) or in a kappacarrageenan gel layer (sandwiched between an external gas permeable membrane and an internal cellulose acetate membrane), coupled to an O 2 amperometric transducer [53]. This biosensor was applied to check the free radical concentration of extra virgin olive oil [54].…”