Superoxide Dismutase Multigene Family from a Primitive Chondrostean Sturgeon, Acipenser baerii: Molecular Characterization, Evolution, and Antioxidant Defense during Development and Pathogen Infection

Kim, Chan‐Hee; Kim, Eun Jeong; Nam, Yoon Kwon

doi:10.3390/antiox10020232

Cited by 12 publications

(5 citation statements)

References 115 publications

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“…Interestingly, SOD has three isoforms which are characterised by cellular localization and metal co-factors such as Zn-Cu-SOD. It is present in the cytosol as SOD-1, second is Mn dependent SOD located in mitochondria (Mn-SOD) called as SOD-2 and last one extracellular Cu/Zn SOD, located within the extracellular matrices of tissues (EC-SOD; SOD-3) 62 , 63 . SOD is also playing crucial roles for development and maintenance of a homeostatic balance between the innate immune response and antioxidant defenses 64 .…”

Section: Discussionmentioning

confidence: 99%

Nano‑zinc enhances gene regulation of non‑specific immunity and antioxidative status to mitigate multiple stresses in fish

Kumar

Singh

Chandan

et al. 2023

Sci Rep

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The toxicity of ammonia surged with arsenic pollution and high temperature (34 °C). As climate change enhances the pollution in water bodies, however, the aquatic animals are drastically affected and extinct from nature. The present investigation aims to mitigate arsenic and ammonia toxicity and high-temperature stress (As + NH3 + T) using zinc nanoparticles (Zn-NPs) in Pangasianodon hypophthalmus. Zn-NPs were synthesized using fisheries waste to developing Zn-NPs diets. The four isonitrogenous and isocaloric diets were formulated and prepared. The diets containing Zn-NPs at 0 (control), 2, 4 and 6 mg kg−1 diets were included. Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-s-transferase (GST) were noticeably improved using Zn-NPs diets in fish reared under with or without stressors. Interestingly, lipid peroxidation was significantly reduced, whereas vitamin C and acetylcholine esterase were enhanced with supplementation of Zn-NPs diets. Immune-related attributes such as total protein, globulin, albumin, myeloperoxidase (MPO), A:G ratio, and NBT were also improved with Zn-NPs at 4 mg kg−1 diet. The immune-related genes such as immunoglobulin (Ig), tumor necrosis factor (TNFα), and interleukin (IL1b) were strengthening in the fish using Zn-NPs diets. Indeed, the gene regulations of growth hormone (GH), growth hormone regulator (GHR1), myostatin (MYST) and somatostatin (SMT) were significantly improved with Zn-NPs diets. Blood glucose, cortisol and HSP 70 gene expressions were significantly upregulated by stressors, whereas the dietary Zn-NPs downregulated the gene expression. Blood profiling (RBC, WBC and Hb) was reduced considerably with stressors (As + NH3 + T), whereas Zn-NPs enhanced the RBC, WBC, and Hb count in fish reread in control or stress conditions. DNA damage-inducible protein gene and DNA damage were significantly reduced using Zn-NPs at 4 mg kg−1 diet. Moreover, the Zn-NPs also enhanced the arsenic detoxification in different fish tissues. The present investigation revealed that Zn-NPs diets mitigate ammonia and arsenic toxicity, and high-temperature stress in P. hypophthalmus.

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Section: Discussionmentioning

confidence: 99%

Nano‑zinc enhances gene regulation of non‑specific immunity and antioxidative status to mitigate multiple stresses in fish

Kumar

Singh

Chandan

et al. 2023

Sci Rep

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“…However, our study did not observe a complete agreement between the level of antioxidant enzyme activity and its gene expression level. Researchers have reported that there is no strictly linear relationship between them [ 55 ]. Therefore, further studies are needed regarding the regulatory relationship between enzyme activity and genes.…”

Section: Discussionmentioning

confidence: 99%

ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

et al. 2022

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Streptococcus agalactiae is common pathogenic bacteria in aquaculture and can cause mass mortality after fish infection. This study aimed to investigate the effects of S. agalactiae infection on the immune and antioxidant regulatory mechanisms of golden pompano (Trachinotus ovatus). Serum and liver samples were obtained at 0, 6, 12, 24, 48, 96, and 120 h after golden pompano infection with S. agalactiae for enzyme activity and gene expression analyses. After infection with S. agalactiae, the content of reactive oxygen species (ROS) in serum was significantly increased (p < 0.05). Serum levels of glucose (GLU), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) increased and then decreased (p < 0.05), reaching a maximum at 6 h. Serum antioxidant enzyme (LZM) activity increased significantly (p < 0.05) and reached a maximum at 120 h. In addition, the mRNA expression levels of antioxidant genes (SOD, CAT, and GPx) in the liver increased and then decreased, reaching the maximum at 24 h, 48 h, and 24 h, respectively. During the experimental period, the mRNA expression levels of NF-κB-related genes of the inflammatory signaling pathway inhibitory κB (IκB) showed an overall decreasing trend (p < 0.05) and the lowest expression at 120 h, whereas the mRNA expression levels of tumor necrosis factor α (TNF-α), interleukin-1β (IL-1β), IκB kinase (IKK), and nuclear factor NF-κB increased significantly (p < 0.05) and the highest expression was at 120 h. In conclusion, these results showed that S. agalactiae could activate internal regulatory signaling in the liver of golden pompano to induce defense and immune responses. This study is expected to lay a foundation to develop the healthy aquaculture of golden pompano and promote a more comprehensive understanding of its disease resistance mechanisms.

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“…Excess formation of reactive oxygen species (ROS) is partly responsible for the methotrexate induced liver injury. Fortunately, liver cells have both enzymatic and non-enzymatic defenses against ROS to maintain the redox balance (C. H. Kim, Kim, and Nam 2021). Superoxide dismutases (SODs) are a family of enzymes that act as the first line of defense against ROS in the enzymatic defense system.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin affects oxidative stress biomarkers in methotrexate-induced hepatotoxicity

Hamdan

Kamal

Waheed

2022

ijhs

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Methotrexate is a chemotherapeutic and immunosuppressive drug used in the treatment of cancer, psoriasis, rheumatoid arthritis and several other disorders. Its use predisposes to hepatotoxicity which is a serious side effect. The current study aimed to assess the oxidative-stress-causing potential of methotrexate to liver cells and evaluate the hepatoprotective activity of the potent antioxidant astaxanthin, by affecting the oxidative stress biomarkers GSH (glutathione), SOD (superoxide dismutase), CAT (catalase) and MDA (malondialdehyde). A model of methotrexate-induced liver toxicity was employed on male rats. Thirty-six rats were divided into six groups; a negative control group, methotrexate induction group given (20 mg/kg) I.P. on day 13, three groups pretreated with oral astaxanthin in ascending doses (50, 75 and 100 mg/kg) for 14 days before methotrexate, and a conventional therapy group pretreated with silymarin (200mg/kg). The use of methotrexate significantly decreased liver tissue GSH, SOD and CAT while significantly increasing MDA, compared to the negative control. On the other side, astaxanthin used in all three doses significantly normalized these biomarkers. This study revealed that since astaxanthin significantly increased GSH, SOD and CAT while decreasing MDA that minimizes oxidative stress, it could be used as pretreatment in patients treated with methotrexate to decrease its hepatotoxicity.

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Superoxide Dismutase Multigene Family from a Primitive Chondrostean Sturgeon, Acipenser baerii: Molecular Characterization, Evolution, and Antioxidant Defense during Development and Pathogen Infection

Cited by 12 publications

References 115 publications

Nano‑zinc enhances gene regulation of non‑specific immunity and antioxidative status to mitigate multiple stresses in fish

Nano‑zinc enhances gene regulation of non‑specific immunity and antioxidative status to mitigate multiple stresses in fish

ROS Induced by Streptococcus agalactiae Activate Inflammatory Responses via the TNF-α/NF-κB Signaling Pathway in Golden Pompano Trachinotus ovatus (Linnaeus, 1758)

Astaxanthin affects oxidative stress biomarkers in methotrexate-induced hepatotoxicity

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