Superparamagnetic lysozyme surface-imprinted polymer prepared by atom transfer radical polymerization and its application for protein separation

The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation

“…When magnetic separation was integrated with imprinted polymer, the resulting magnetically imprinted polymer can not only selectively recognize the target proteins in complex matrix but also can be easily collected and separated by an external magnetic field [18,[21][22][23]. In our previous work [24], the atom transfer radical polymerization (ATRP) has been first developed as a new imprinting technology on magnetic supports to prepare small protein Lyz surface-imprinted polymer. ATRP is a new class of controlled living radical polymerization, possesses the mild reaction conditions without heating and ultraviolet radiations and avoids the adverse reactions comparing with traditionally initiated radical polymerization.…”

Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

“…The solvent flow rate was set at 1 mL/min with solvent A increasing from 37.5 to 62.5 vol% in 20 min for proteins mixture and from 20 to 100 vol% in 80 min for real human serum. The analyte injection volume was 20 L, and the column temperature was set at 35 • C. The samples were analyzed by a UV detector at a wavelength of 214 nm [21].…”

“…On the other hand, when adding the mixture of PEG and sulphate to magnetic particles, the adsorbed proteins would be eluted out and enriched. Here, ATPS was as elution solvent, which had been reported in our previous work [21].…”

“…Magnetic cation exchange adsorbents were introduced into a micellar ATPS by Becker et al [20], which made the phase selectivity of lysozyme (Lyz) be effectively inverted and obtained Lyz in a high yield from the mixed solution of Lyz and ovalbumin. In our previous work [21], ATPE was used as a novel elution solution to achieve adsorbed target Lyz from imprinted polymer modified magnetic particles, which exhibits the elution, extraction and enrichment effect to adsorbed proteins. The integration of mag-0039-9140/$ -see front matter © 2011 Elsevier B.V. All rights reserved.…”

Electrochemical template synthesis of protein-imprinted magnetic polymer microrods

“…As a consequence of the procedure the magnet is fully encapsulated by the thin imprinted polymer layer, thereby leading to high incorporation efficiency [28][29][30][31][32][33][34][35][36][37].…”