In the present study, artificial neural networks (ANNs) were used to model flow stress in Ti-6Al-4V alloy with equiaxed and Widmanstä tten microstructures as initial microstructures. Continuous compression tests were performed on a Gleeble 3500 thermomechanical simulator over a wide range of temperatures (700-1100uC) with strain rates of 0?001-100 s 21 and true strains of 0?1-0?6. These tests have been focused on obtaining flow stress data under varying conditions of strain, strain rate, temperature, and initial microstructure to train ANN model. The feed forward neural network consisted of two hidden layers with a sigmoid activation function and backpropagation training algorithm was used. The architecture of the network includes four input parameters: strain rate : e, temperature T, true strain e and initial microstructure and one output parameter: the flow stress. The initial microstructure was considered qualitatively. The ANN model was successfully trained across (azb) to b phase regimes and across different deformation domains for both of the microstructures. Results show that the ANN model can correctly reproduce the flow stress in the sampled data and it can predict well with the nonsampled data. A graphical user interface was designed for easy use of the model.