Superstimulation of Follicular Growth in Thai Native Heifers by a Single Administration of Follicle Stimulating Hormone Dissolved in Polyvinylpyrrolidone

Abstract

This study was undertaken to determine whether a single i.m. injection of FSH dissolved in 10 ml of 30% (wt/vol) polyvinylpyrrolidone (PVP; MW=40,000) to form FSHp would induce follicular growth in Thai native heifers and to determine its optimal dose. In Group 1, heifers (n=4) were given multiple i.m. injections of FSHp every 12 h for 3 days at decreasing doses, for a total of 100 mg (control). In Groups 2, 3 and 4, heifers (n=4 in each group) were given single i.m. injections of FSHp at 50, 100 and 150 mg. All heifers received a single injection of 750 µg PGF2α 48 h after the initiation of exogenous FSH treatment. Ovaries of treated heifers were examined by transrectal ultrasonography every day until they showed estrus. Group 3 showed significantly higher numbers of ovulation follicles, significantly higher growth rates of follicles per day and significantly larger diameters of follicles and corpora lutea than groups 1 and 2 but not Group 4 (P<0.05). Group 4 showed significantly higher numbers of large follicles (≥5 mm in diameter), unovulated follicles and ovulations, a significantly higher growth rate of follicles per day, and significantly larger diameters of follicles and corpora lutea (P<0.05) than those of the other groups. This indicates a state of overstimulation of ovaries in this group. Besides, the plasma levels of FSH in Group 4 were significantly higher (P<0.05) than in the other group and were maintained in the range of 2.2–0.7 ng/ml over a period of 6 to 66 h after the FSHp injection. Meanwhile, the plasma levels of P4 and E2 did not differ in any of the groups in the period of 0 to 96 h during the superstimulation program. In conclusion, it was demonstrated that a single i.m. injection of 100 mg FSHp was the most effective dose for superstimulation of follicular growth in Thai native heifers under the experimental conditions in this study.

Efficacy of a single intramuscular injection of porcine FSH in hyaluronan prior to ovum pick-up in Holstein cattle

“…On the basis of the rapid clearance of FSH (w5 hours) [39], regardless of the dose administered [55], an alternative could be to modify hormone absorption. Modifications examined have included changing the route administration (subcutaneous injection, [55][56][57]) or mixing the pFSH with polymers, such as polyvinylpyrrolidone [58], aluminum hydroxide gel [59] or HA [42] to reduce the rate of absorption. Tríbulo et al [42] reported satisfactory superovulatory results after treating Table 1 Summary of cumulus-oocyte complex yield and in vitro embryo production (mean AE standard error or percentage) in nonlactating Holstein cows that were not treated (control), treated with 200 mg of porcine FSH (pFSH; F200) in twice-daily injections or 200 or 300 mg of pFSH as a single injection in 0.5% hyaluronan (F200HA and F300HA).…”

A simplified superovulation protocol using split-single administration of Folltropin®-V in hyaluronan: application to purebred sheep

“…For example, a single superovulation injection with FSH in degradable polymers to sustain hormone release has been studied in ruminants (Lopez-Sebastian et al 1993;Yamamoto et al 1994;Satoh et al 1996;Sugano and Shinogi 1999;D'Alessandro et al 2001;Kimura et al 2007;Tribulo et al 2012). In cattle, administration of FSH dissolved in various substances, including polyvinylpyrrolidone, aluminum hydroxide gel https://doi.org/10.17221/52/2016-VETMED or hyaluronan (HA), increased ovarian responses compared to multiple FSH injections (Bo et al 1994;Yamamoto et al 1995;Kimura et al 2007;Tribulo et al 2012;Chasombat et al 2013). HA is a biological polymer that is abundantly expressed in many tissues.…”

Superovulation in cattle – searching for the optimal dose, alternative routes of administration and a simplified FSH application program

“…The FSH suspension in PVP characterized with high viscosity, which created some difficulties to achieve a homogeneous mixture and limited its practical application (6,23). However, as no other undesirable effects have been demonstrated, PVP is still used in some countries as an adjuvant in commercial FSH preparations (10).…”