Professional nurses, one of the human resources in the health field, have an obligation to carry out the nursing process, especially nursing based on scientific evidence. This study’s aim was to describe the knowledge, attitudes, implementation towards evidence-based practice and its barriers in the Hospital. A quantitative research with descriptive approach was conducted in the Dr. M. Djamil Hospital Padang. A consecutive sampling technique was utilized with 139 respondents being selected and only 90 respondents completely filled in the questionnaires. The instruments used were Evidence-Based Practice Questionnaire (EBPQ) and BARRIERS Scale. Characteristics of respondents were 70.0% diploma education, mean (SD) age was 36.7 (7.95) years and 13.35 (8.37) years working time. The average EBP-related attribute score was higher than the average score of EBP knowledge/understanding, understanding of research terms, confidence and practice towards EBP. There is a need to improve knowledge and understanding of EBP and research and overcome the obstacles of EBP implementation in the practice of nursing service.