Superwettable microchips with improved spot homogeneity toward sensitive biosensing

Chen, Yanxia; Xu, Li-Ping; Meng, Jing; Deng, Shaohui; Ma, Lulin; Zhang, Shudong; Zhang, Xueji; Wang, Shutao

doi:10.1016/j.bios.2017.11.036

Cited by 56 publications

(45 citation statements)

References 50 publications

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“…For example, Chen et al presented superwettable microchips for sensitive biosensing of prostatespecific antigen (f-PSA), as shown in Figure 11a. [196] The analyte droplets would be anchored on the superhydrophilic spots and the analytes could distribute homogeneously on the region, which could be attributed to the enhanced Marangoni effect of superwetting region and the suppressed outward flow by nanodendritic silica architecture. To verify the feasibility in biosensing, f-PSA was chosen to demonstrate the proof of concept.…”

Section: Protein Detectionmentioning

confidence: 99%

“…To solve these, researchers have dedicated to developing biomaterials with specific wettability, together with adding antibacterial ingredients for further treatment. Up to date, medical dressings based on biomaterials with specific wettability have demonstrated values in accelerating the wound healing process through removing excessive biofluid around wounds, [ 96 , 174 ] inhibiting adhesion of harmful biomolecules, [ 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 , 186 , 187 , 188 , 189 , 190 , 191 , 192 , 193 , 194 , 195 , 196 , 197 , 198 , 199 , 200 , 201 , 202 , 203 , 204 , 205 , 206 , 207 , 208 , 209 , 210 , 211 , 212 , 213 , 214 , 215 , 216 , 217 , 218 , 219 ,…”

Section: Biomaterials and Tissue Engineeringmentioning

confidence: 99%

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Tailoring Materials with Specific Wettability in Biomedical Engineering

et al. 2021

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As a fundamental feature of solid surfaces, wettability is playing an increasingly important role in our daily life. Benefitting from the inspiration of biological paradigms and the development in manufacturing technology, numerous wettability materials with elaborately designed surface topology and chemical compositions have been fabricated. Based on these advances, wettability materials have found broad technological implications in various fields ranging from academy, industry, agriculture to biomedical engineering. Among them, the practical applications of wettability materials in biomedical-related fields are receiving remarkable researches during the past decades because of the increasing attention to healthcare. In this review, the research progress of materials with specific wettability is discussed. After briefly introducing the underlying mechanisms, the fabrication strategies of artificial materials with specific wettability are described. The emphasis is put on the application progress of wettability biomaterials in biomedical engineering. The prospects for the future trend of wettability materials are also presented.

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Section: Protein Detectionmentioning

confidence: 99%

Section: Biomaterials and Tissue Engineeringmentioning

confidence: 99%

Tailoring Materials with Specific Wettability in Biomedical Engineering

et al. 2021

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“…Based on this functional surface, the detection of microRNA‐141 could be achieved at an extreme low limit of 1 pM, which was also successful even in real samples. Wang's group fabricated a series of superwettable microchips, possessing the abilities of detecting the biomolecules miRNA‐141, 103 DNA, 104 free prostate‐specific antigen 105 with respective detection limit of 88 pM, 2.3 × 10 −16 M, 10 fg/mL.…”

Section: Bioinspired Superwetting Surfaces For Biosensingmentioning

confidence: 99%

mentioning

confidence: 99%

Bioinspired superwetting surfaces for biosensing

Zhu

Huang

Lou

et al. 2020

VIEW

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Inspired by nature, scientists and researchers have studied the wetting behaviors on various creatures and mimicked their structures to fabricate diverse functional superwetting materials. As one kind of emerging application, the bioinspired superwettable surfaces used for biosensing have been aroused wide interests. In this review, we summarized the recent developments of bioinspired superwettable surfaces in the field of biosensing. In the first part, superwettable creatures in nature, namely, superhydrophobic self-cleaning lotus leaf, high-adhesion superhydrophobic rose petal, amphiphobic springtail, patterned wetting desert beetles, slippery pitcher plant, were introduced. In sequence, we successively described the special wetting models of superhydrophobicity, superamphiphobicity, responsive wettability, patterned wettability, and slipperiness. Then, biosensing applications based on the respective patterned wettable, superhydrophobic, responsive wettable, and slippery substrates that were combined with the common detection approaches (colorimetry, fluorescence, surface-enhanced Raman scattering (SERS), electrochemistry) were shown in detailed. At last, an insight of remaining challenges and future development for bioinspired superwetting materials applied in biosensing was provided.

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“…[20][21][22] Moreover, printing of antibodies on the microarray surface can introduce issues such as non-uniform surface coverage and assay-to-assay variability, affecting the assay accuracy and reproducibility. [23][24][25] A DNA-based site-specific antibody immobilization technique, known as DNA-directed immobilization (DDI), has gained interest due to the reproducible production of DNA microarrays, compatibility of DNA microarrays with the fabrication of integrated microfluidic systems, and stability and robustness of DNA chips. In this technique, a universal ssDNA chip is first converted to an antibody microarray using antibodies tagged with short DNA sequences complementary to the immobilized DNA capture probes ( Figure 1).…”

mentioning

confidence: 99%

Configurable Digital Virus Counter on Robust Universal DNA Chips

Seymour

Ünlü

Carter

et al. 2020

Preprint

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Here, we demonstrate real-time multiplexed virus detection by applying DNA-directed antibody immobilization technique to a single-particle interferometric reflectance imaging sensor (SP-IRIS). In this technique, the biosensor chip surface spotted with different DNA sequences is converted to a multiplexed antibody array by flowing antibody-DNA conjugates and allowing specific DNA-DNA hybridization. The resulting antibody array is shown to detect three different recombinant Vesicular Stomatitis Viruses (rVSVs) genetically engineered to express surface glycoproteins of Ebola, Marburg, and Lassa viruses in real-time in a disposable microfluidic cartridge. We also show that this method can be modified to produce a single-step, homogeneous assay format by mixing the antibody-DNA conjugates with the virus sample in solution phase prior to flowing in the microfluidic cartridge, eliminating the antibody immobilization step. This homogenous approach achieved detection of the model Ebola virus, rVSV-EBOV, at a concentration of 100 PFU/ml in 1 hour. Finally, we demonstrate the feasibility of this homogeneous technique as a rapid test using a passive microfluidic cartridge. A concentration of 104 PFU/ml was detectable under 10 minutes for the rVSV-Ebola virus. Utilizing DNA microarrays for antibody-based diagnostics is an alternative approach to antibody microarrays and offers advantages such as configurable sensor surface, long-term storage ability, and decreased antibody use. We believe these properties will make SP-IRIS a versatile and robust platform for point-of-care diagnostics applications.

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Superwettable microchips with improved spot homogeneity toward sensitive biosensing

Cited by 56 publications

References 50 publications

Tailoring Materials with Specific Wettability in Biomedical Engineering

Tailoring Materials with Specific Wettability in Biomedical Engineering

Bioinspired superwetting surfaces for biosensing

Configurable Digital Virus Counter on Robust Universal DNA Chips

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