Supplemental data from A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise

Abstract

<p>Figure S1. Chemical structures of MMAF, MMAE and 13C5-MMAE. The asterisks indicate where Carbon-13 isotope has replaced Carbon-12; Figure S2. Expression of CD32 on mature neutrophils. Circulating neutrophils (AllCell) were incubated with anti-CD32 antibody conjugated with FITC (CD32-FITC) or control IgG1-FITC, and were subjected to FACS analysis. Typical results from one experiment were shown; Figure S3. Little expression of SLC44A4 and ENPP3 on neutrophils at different differentiation stages. Microarray analyses were done for SLC44A4 (AGS5 target) or ENPP3 (AGS16 target) in HSCs, differentiating neutrophils at Day 8 or Day 14, or circulating neutrophils (mature); Figure S4. AGS5-vcMMAF conjugation did not affect intracellular cleavage. PC3 cells overexpressing SLC44A4, PC3-SLC44A4 were incubated with indicated concentrations of AGS5-vcMMAE, AGS5-vcMMAF or AGS5-mcMMAF for 6 days; Table S1: Information about ADCs used in this report; Table S2: Chromatographic gradient; Table S3: MRM Transitions monitored; Table S4. CD66b expression during neutrophil differentiation; Table S5. Correlation between sensitivity and extracellular cleavage.</p>