Supplemental Oxygen Alters the Airway Microbiome in Cystic Fibrosis

Vieira, Jacob; Jesudasen, Sirus; Bringhurst, Lindsay; Sui, Hui-Yu; McIver, Lauren J.; Whiteson, Katrine; Hanselmann, Kurt; O’Toole, George A.; Richards, Christopher J.; Sicilian, Leonard; Neuringer, Isabel P.; Lai, Peggy S.

doi:10.1128/msystems.00364-22

Cited by 6 publications

(5 citation statements)

References 84 publications

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“…Hyperoxia exposure and OVA sensitization can exert an effect on the lung microflora. Hyperoxia exposure can alter the lung microbiota ( Vieira et al, 2022 ), and dysbiosis of the lung microbiota can cause acute lung injury, wherein oxygen-induced changes in the lung microbiota precede the development of acute lung injury, and germ-free mice (mice without microorganism exposure) are protected from an oxygen-induced acute lung injury, suggesting that the deleterious effects of hyperoxia on lung injury are, at least partially, mediated by the lung microbiota ( Ashley et al, 2020 ). Once established, the establishment of nonbeneficial respiratory microbiota triggers a self-reinforcing cycle of pro-inflammatory pathways that alter the respiratory microenvironment and may lead to long-term sequelae, including asthma and chronic obstructive ( de Steenhuijsen Piters et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of early postnatal hyperoxia exposure combined with early ovalbumin sensitization on lung inflammation and bacterial flora in a juvenile mouse model of asthma

Bao

Cao

et al. 2023

Front. Microbiol.

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ObjectiveThe aim of this study is to explore the effects of early postnatal hyperoxia exposure combined with early ovalbumin (OVA) sensitization on lung inflammation and bacterial flora in neonatal mice on a juvenile mouse model of asthma.MethodsThirty-two newborn female C57BL/6 J mice were randomly divided into four groups, which including room air+phosphate-buffered saline (PBS) group, hyperoxia+PBS group, room air+OVA group, and hyperoxia+OVA group, according to the hyperoxia exposure and/or OVA induction. Mice were exposed to either 95% O2 or room air for 7 days after birth; after 7 days, they were exposed to air and received an intraperitoneal injection of OVA suspension or PBS solution on postnatal days 21 (P21) and 28 (P28). From P36 to P42, the mice were allowed to inhale of 1% OVA or 0.9% NaCl solution. The mice were observed after the last excitation. HE staining was performed to observe the pathological changes in lung tissues. Wright-Giemsa staining was used to perform bronchoalveolar lavage fluid (BALF) leukocyte sorting. Enzyme-linked immunosorbent assay was used to determined the cytokines levels of interleukin (IL)-2, IL-5, IL-13, IL-17A, and IL-10 and serum IgE levels in BALF. Additionally, 16S rRNA sequencing was used to analyze the characteristics of lung microbiota.ResultsMice in the hyperoxia+OVA group showed asthma-like symptoms. HE staining results revealed a significant thickening of the airway wall and airway inflammation. BALF analysis of cellular components showed significant increases in total leukocyte and eosinophil counts and the levels of cytokines related to Th2 (IL-5 and IL-13) and Th17 (IL-17A); 16S rRNA sequencing revealed that the main members of the pulmonary microflora were Actinobacteriota, Proteobacteria, Firmicutes, and Bacteroidota at the phylum level. In addition, the bacteria with a major role were Acinetobacter and Moraxellaceae in the O2 + OVA group.ConclusionThe mouse suffering from postnatal hyperoxia exposure and early OVA sensitization, changes in symptoms, pathology, leukocyte and eosinophil counts, and levels of different T-cell cytokines in BALF and lung microbiota, which may provide a basis for the establishment of a juvenile mouse model of asthma.

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Section: Discussionmentioning

confidence: 99%

Effects of early postnatal hyperoxia exposure combined with early ovalbumin sensitization on lung inflammation and bacterial flora in a juvenile mouse model of asthma

Bao

Cao

et al. 2023

Front. Microbiol.

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“…Four aliquots were made with one nasal swab sample, and a swab and 200 uL of sample solution was utilized for each aliquot. Sputum was collected from adult patients with cystic brosis described in a previous study [29,30]. Brie y, adult persons over age 18 satisfying cystic brosis clinical diagnostic criteria and receiving routine care at the Massachusetts General Hospital Adult Cystic Fibrosis Center were recruited.…”

Section: Sample Collectionmentioning

confidence: 99%

Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies

Kim,

II,

Shah

et al. 2024

Preprint

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Background Most respiratory microbiome studies have focused on amplicon rather than metagenomics sequencing due to high host DNA content. We evaluated efficacy of five host DNA depletion methods on previously frozen human bronchoalveolar lavage (BAL), nasal swabs, and sputum prior to metagenomic sequencing. Results Median sequencing depth was 76.4 million reads per sample. Untreated nasal, sputum and BAL samples had 94.1%, 99.2%, and 99.7% host-reads. The effect of host depletion differed by sample type. Most treatment methods increased microbial reads, species richness and predicted functional richness; the increase in species and predicted functional richness was mediated by higher effective sequencing depth. For BAL and nasal samples, most methods did not change Morisita-Horn dissimilarity suggesting limited bias introduced by host depletion. Conclusions Metagenomics sequencing without host depletion will underestimate microbial diversity of most respiratory samples due to shallow effective sequencing depth and is not recommended. Optimal host depletion methods vary by sample type.

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“…Были отмечены достоверные значимые изменения в сетях микробного взаимодействия. Воздействие гипероксии нарушает микробные сообщества дыхательных путей с доминированием основных патогенов [29].…”

Section: результаты исследования и их обсуждениеunclassified

Effect of Hyperoxic Exposure on the Respiratory and Intestinal Microbiome

Khomyakova,

Makarova,

Mkhitarov

et al. 2023

НО МН (SR MS)

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Гипероксия как системное воздействие на организм человека должна обеспечивать повышенное содержание кислорода в тканях организма. Гипероксия создается с лечебной целью при кислородной терапии, однако при продолжительной гипероксии развивается кислородное отравление. Более того, в последние годы было показано, что при использовании систем искусственной вентиляции легких повышается частота инфекционных заболеваний дыхательной системы и смертность больных. Важнейшим компонентом поддержания здоровья человека является нормобиом, то есть микробное сообщество, обеспечивающее жизненно важные функции организма хозяина. При глубоком повреждении микробиома, в том числе под действием высоких доз кислорода, в результате реализации механизмов поддержания микробного гомеостаза может сформироваться патобиом, который характеризуется стабильностью соотношения микробных компонентов при высоком содержании патогенных микроорганизмов и пониженном количестве «полезных» бактерий. Целью настоящего исследования была систематизация данных по воздействию гипероксии на респираторный и кишечный микробиом. В результате проведенного анализа литературы сделано заключение о том, что респираторный и кишечный микробиом представляют собой важный элемент в патогенезе повреждений, вызванных гипероксией. Предсуществующее наличие дисбиотических изменений должно рассматриваться как фактор риска развития осложнений, вызванных гипероксией. Ключевые слова: респираторный микробиом, кишечный микробиом, гипероксия, патобиом, искусственная вентиляция легких, критические больныеРабота выполнена при финансировании и в рамках выполнения государственного задания FURG 2023 0038.

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Supplemental Oxygen Alters the Airway Microbiome in Cystic Fibrosis

Abstract: The airway microbiome in persons with cystic fibrosis (pwCF) is correlated with lung function and disease severity. Supplemental oxygen use is common in more advanced CF, yet its role in perturbing airway microbial communities is unknown.

Cited by 6 publications

References 84 publications

Effects of early postnatal hyperoxia exposure combined with early ovalbumin sensitization on lung inflammation and bacterial flora in a juvenile mouse model of asthma

Effects of early postnatal hyperoxia exposure combined with early ovalbumin sensitization on lung inflammation and bacterial flora in a juvenile mouse model of asthma

Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies

Effect of Hyperoxic Exposure on the Respiratory and Intestinal Microbiome

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