Supplementary Ultraviolet Radiation B Together with Blue Light at Night Increased Quercetin Content and Flavonol Synthase Gene Expression in Leaf Lettuce (Lactuca sativa L.)

Ebisawa, Masanori; Shoji, K.; Kato, Macky; Shimomura, Koichiro; Goto, Fumiyuki; Yoshihara, Toshihiro

doi:10.2525/ecb.46.1

Cited by 66 publications

(39 citation statements)

References 38 publications

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“…White LEDs containing the blue wavelength range also induced higher values in the above variables than did the red and green LED treatments and the control light. Because the wavelength of the blue light is close to that of ultraviolet light, irradiation under the blue LEDs is expected to have a similar response to that of ultraviolet light, which triggers the biosynthesis of phenolic compounds with antioxidant properties (Ebisawa et al, 2008;Ryan et al, 2002). Total phenolic concentration and total flavonoid concentration in seedlings grown under the green LEDs were not significantly different from those under control light, but the LED treatment showed 82.5% lower antioxidant capacity than control seedlings.…”

Section: Resultsmentioning

confidence: 96%

Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes

Kim

Park

et al. 2014

Hortic. Environ. Biotechnol.

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Light-emitting diodes (LEDs) can be used in closed-type plant production systems as an artificial light source. Here, we determined the effects of monochromatic LEDs on the growth and production of phenolic antioxidants in cherry tomato seedlings (Solanum lycopersicum L. 'Cuty'). Two week-old seedlings germinated under normal growing conditions were transplanted into a growth chamber equipped with various monochromatic LEDs and fluorescent lamps (control), and cultivated for 4 weeks. Fresh weights of shoots and roots under LED treatment, especially, red or green, were higher than those under the control light at 4 weeks. The SPAD value of seedlings grown under blue LEDs was significantly lower than in seedlings grown under other LEDs. The plant height, stem length, and internode length of tomato seedlings grown under blue LEDs were the highest. Blue LEDs induced 1.5-2.2-fold higher stem length than red and white LEDs. Expansin gene expression was the highest under blue LEDs, consistent with the effect on stem length. Blue LEDs stimulated the biosynthesis of total phenolics, antioxidants, and total flavonoids in tomato seedlings. Specifically, the antioxidant capacity of seedlings grown under blue LEDs was 2.1-folds higher than that in seedlings grown under green LEDs. Thus, manipulating light quality using LEDs is a crucial factor for growth and antioxidant production in cherry tomato seedlings.

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Section: Resultsmentioning

confidence: 96%

Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes

Kim

Park

et al. 2014

Hortic. Environ. Biotechnol.

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“…Within a head-type lettuce plant, the phytochemical content varies, with the outer leaves having higher phenolic content and lipophilic antioxidant activity than inner leaves. Genes encoding many of these compounds in lettuce are regarded as generally being light regulated (Ebisawa et al 2008;Kleinhenz et al 2003;Oh et al 2009a;Park et al 2007), but direct evidence exists only for a few genes in these biosynthetic and regulatory pathways. Experimental evidence from Arabidopsis suggests that this supposition may be an oversimplification, as several genes of the phenylpropanoid pathway do not respond to light (Hemm et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Mapping QTL, epistasis and genotype × environment interaction of antioxidant activity, chlorophyll content and head formation in domesticated lettuce (Lactuca sativa)

et al. 2012

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Fruits and vegetables are rich sources of antioxidants in human diets and their intake is associated with chronic disease prevention. Lettuce (Lactuca sativa L.) is a common vegetable in diets worldwide, but its nutritional content is relatively low. To elucidate the genetic basis of antioxidant content in lettuce, we measured the oxygen radical absorbance capacity (ORAC) and chlorophyll (Chl) content as a proxy of β-carotene in an F(8) recombinant inbred line (RIL) in multiple production cycles at two different production sites. Plants were phenotyped at the open-leaf stage to measure genetic potential (GP) or at market maturity (MM) to measure the influence of head architecture ('head' or 'open'). Main effect quantitative trait loci (QTL) were identified at MM (three Chl and one ORAC QTL) and GP (two ORAC QTL). No main effect QTL for Chl was detected at GP, but epistatic interaction was identified in one pair of marker intervals for each trait at GP. Interactions with environment were also detected for both main and epistatic effects (two for main effect, and one for epistatic effect). Main effect QTL for plant architecture and nutritional traits at MM colocated to a single genomic region. Chlorophyll contents and ORAC values at MM were significantly higher and Chl a to Chl b ratios were lower in 'open' types compared to 'head' types. The nutritional traits assessed for GP showed a significant association with plant architecture suggesting pleiotropic effects or closely linked genes. Taken together, the antioxidant and chlorophyll content of lettuce is controlled by complex mechanisms and participating alleles change depending on growth stage and production environment.

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“…Target gene sequences corresponding to the top BLAST hits were identified within the Compositae Genome Project EST database via sequence homology to known light absorption and transfer genes from existing L. sativa L. sequence data in GenBank. Primers of the L. sativa L. ACTIN gene (Accession number: AB359898.1) reported by Ebisawa et al () were used. The primers for Psb A and LHCb were designed by Primer‐Premier 6.0 (Biosoft International, Palo, CA).…”

Section: Methodsmentioning

confidence: 99%

Study of the beneficial effects of green light on lettuce grown under short‐term continuous red and blue light‐emitting diodes

Bian

Yang

et al. 2018

Physiologia Plantarum

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Red and blue light are the most important light spectra for driving photosynthesis to produce adequate crop yield. It is also believed that green light may contribute to adaptations to growth. However, the effects of green light, which can trigger specific and necessary responses of plant growth, have been underestimated in the past. In this study, lettuce (Lactuca sativa L.) was exposed to different continuous light (CL) conditions for 48 h by a combination of red and blue light-emitting diodes (LEDs) supplemented with or without green LEDs, in an environmental-controlled growth chamber. Green light supplementation enhanced photosynthetic capacity by increasing net photosynthetic rates, maximal photochemical efficiency, electron transport for carbon fixation (J ) and chlorophyll content in plants under the CL treatment. Green light decreased malondialdehyde and H O accumulation by increasing the activities of superoxide dismutase (EC 1.15.1.1) and ascorbate peroxidase (EC 1.11.1.11) after 24 h of CL. Supplemental green light significantly increased the expression of photosynthetic genes LHCb and PsbA from 6 to 12 h, and these gene expressions were maintained at higher levels than those under other light conditions between 12 and 24 h. However, a notable downregulation of both LHCb and PsbA was observed during 24 to 48 h. These results indicate that the effects of green light on lettuce plant growth, via enhancing activity of particular components of antioxidative enzyme system and promoting of LHCb and PsbA expression to maintain higher photosynthetic capacity, alleviated a number of the negative effects caused by CL.

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Supplementary Ultraviolet Radiation B Together with Blue Light at Night Increased Quercetin Content and Flavonol Synthase Gene Expression in Leaf Lettuce (Lactuca sativa L.)

Cited by 66 publications

References 38 publications

Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes

Growth and antioxidant phenolic compounds in cherry tomato seedlings grown under monochromatic light-emitting diodes

Mapping QTL, epistasis and genotype × environment interaction of antioxidant activity, chlorophyll content and head formation in domesticated lettuce (Lactuca sativa)

Study of the beneficial effects of green light on lettuce grown under short‐term continuous red and blue light‐emitting diodes

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