Supplementation of Bacillus sp. DU-106 Alleviates Antibiotic-Associated Diarrhea in Association with the Regulation of Intestinal Microbiota in Mice

Darong, Huang; Chen, Yanlan; Chen, Hongzhu; Deng, Xu; Huang, Jianzhao; Lu, Shu‐Ling; Li, Ming; Du, Bing

doi:10.1007/s12602-022-09906-8

Cited by 17 publications

(7 citation statements)

References 49 publications

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“…There are currently few reports on Eubacterium_xylanophilum . In a mouse antibiotic-related diarrhea model, the abundance of Eubacterium_xylanophilum decreased; however, it improved after probiotics treatment ( Huang et al, 2022 ). Intragastric administration of Ir-NPs in mice could restore the decline in the abundance of beneficial bacteria caused by acute altitude hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of bioactive iridium nanozymes on high altitude-related hypoxia-induced kidney injury in mice

Wang

Shi²,

Chu³

et al. 2023

Front. Pharmacol.

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Introduction: High altitude-related hypoxia-induced organ damage significantly impacts people who are exposed to acute high-altitude environment. At present, kidney injury still lacks effective treatment strategies. Iridium nanozymes (Ir-NPs) are a nanomaterial with various enzymatic activities and are expected to be used in kidney injury treatment.Methods: In this study, we simulated a high-altitude environment (6000 m) to induce a kidney injury model, and explored the therapeutic effect of Ir-NPs in mice with kidney injury in this environment. Changes in the microbial community and metabolites were analyzed to explore the possible mechanism underlying the improvement of kidney injury during acute altitude hypoxia in mice treated with Ir-NPs.Results: It was discovered that plasma lactate dehydrogenase and urea nitrogen levels were considerably increased in mice exposed to acute altitude hypoxia compared to mice in a normal oxygen environment. Furthermore, there was a substantial increase in IL-6 expression levels in hypoxic mice; contrastingly, Ir-NPs decreased IL-6 expression levels, reduced the levels of succinic acid and indoxyl sulfate in the plasma and kidney pathological changes caused by acute altitude hypoxia. Microbiome analysis showed that bacteria, such as Lachnospiraceae_UCG_006 predominated in mice treated with Ir-NPs.Conclusion: Correlation analysis of the physiological, biochemical, metabolic, and microbiome-related parameters showed that Ir-NPs could reduce the inflammatory response and protect kidney function under acute altitude hypoxia, which may be related to intestinal flora distribution regulation and plasma metabolism in mice. Therefore, this study provides a novel therapeutic strategy for hypoxia-related kidney injury, which could be applied to other hypoxia-related diseases.

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Section: Discussionmentioning

confidence: 99%

Protective effect of bioactive iridium nanozymes on high altitude-related hypoxia-induced kidney injury in mice

Wang

Shi²,

Chu³

et al. 2023

Front. Pharmacol.

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“…It is similar to probiotics Bacillus sp. DU-106 ( 35 ), Lactobacillus reuteri I5007 ( 36 ), and Lactobacillus sakei K040706 ( 37 ) in regulating cytokine levels in diarrhea mice. This provides a basis for the clinical application of the strain 2-33 after days.…”

Section: Discussionmentioning

confidence: 99%

“…The change of intestinal flora is related to the development of intestinal diseases, the ratio of Firmicutes and Bacteroidota is usually used to evaluate various intestinal diseases of patients, such as diarrhea, irritable bowel syndrome, and metabolic diseases and significantly affects the main components of intestinal flora, and this change is mainly caused by the destruction of intestinal microenvironment ( 35 , 42 ). In this study, it was found that the relative abundance of Firmicutes increased, the relative abundance of Bacteroidota decreased, the ratio of Firmicutes and Bacteroidota increased significantly ( p < 0.05), and the relative abundance of phylum also increased in the experimental group after the 14th day gastric perfusion of strain 2-33 compared with the self-healing group.…”

Section: Discussionmentioning

confidence: 99%

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bao

et al. 2022

Front. Nutr.

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Diarrhea is one of the common adverse reactions in antibiotic treatment, which is usually caused by the imbalance of intestinal flora, and probiotics play an important role in the structure of intestinal flora. Therefore, this experiment studied the regulatory effect of Lactiplantibacillus plantarum 2-33 on antibiotic-associated diarrhea (AAD) mice. First, the AAD mice model was established by the mixed antibiotic solution of gentamicin sulfate and cefradine. Then, the physiological indexes and diarrhea of mice were observed and recorded by gastric perfusion of low dose (1.0 × 107 CFU/ml), medium dose (1.0 × 108CFU/ml), and high dose (1.0 × 109 CFU/ml) strain 2-33. 16S rRNA gene V3-V4 regions were sequenced in colon contents of mice in control group, model group, self-healing group, and experimental group, respectively, and the diversity of intestinal flora and gene function prediction were analyzed. The results showed that the intestinal flora of AAD mice was not significantly regulated by gastric perfusion of strain 2-33 to 7 days, but the relative abundance and diversity of intestinal flora of AAD mice were significantly improved by gastric perfusion to 14 days (p < 0.05). In addition, at the genus level, the relative abundance of Lactobacillus increased significantly, and the relative abundance of Enterococcus and Bacillus decreased significantly (p < 0.05). In addition, the regulation of strain 2-33 on intestinal flora of AAD mice was time- and dose-dependent, short-term gastric perfusion, and low dose had no significant effect (p > 0.05). Strain 2-33 can significantly increase the levels of anti-inflammatory cytokines IL-4 and IL-10, significantly decrease the levels of proinflammatory cytokines TNF-α and IFN-γ (p < 0.05), and can also adjust carbohydrate metabolism, amino acid metabolism, and energy metabolism to normal levels, thus accelerating the recovery of intestinal flora structure of AAD mice. In summary, strain 2-33 can improve the structure and diversity of intestinal flora of AAD mice, balance the level of substance and energy metabolism, and play a positive role in relieving diarrhea, maintaining and improving the intestinal microecological balance.

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“…DU‐106 may activate innate immunity by modulating the gut microbiota. Huang et al (2022) also found that Bacillus sp. DU‐106 ameliorates antibiotic‐associated diarrhoea in mice.…”

Section: Introductionmentioning

confidence: 93%

Bacillus sp. DU-106 ameliorates type 2 diabetes by modulating gut microbiota in high-fat-fed and streptozotocin-induced mice

Yan

Xue

et al. 2022

Journal of Applied Microbiology

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Aims: Type 2 diabetes (T2D) is a chronic disease that manifests as endocrine and metabolic disorders that seriously threatening public health. This study aimed to investigate the effects of Bacillus sp. DU-106 on anti-diabetic effects and gut microbiota in C57BL/6J mice fed a high-fat diet and streptozotocin-induced T2D.Methods and Results: Bacillus sp. DU-106 was administered to model mice for eight consecutive weeks. Oral administration of Bacillus sp. DU-106 decreased food and water intake and alleviated body weight loss. Moreover, Bacillus sp. DU-106 imparted several health benefits to mice, including balanced blood glucose, alleviation of insulin resistance in T2D mice and an improvement in lipid metabolism. Furthermore, Bacillus sp. DU-106 protected against liver and pancreatic impairment.Additionally, Bacillus sp. DU-106 treatment reshaped intestinal flora by enhancing gut microbial diversity and enriching the abundance of certain functional bacteria. Conclusion:Collectively, these findings suggest that Bacillus sp. DU-106 can ameliorate T2D by regulating the gut microbiota.Significance and Impact of Study: Therefore, a novel probiotic, Bacillus sp. DU-106 may be a promising therapeutic agent for improving and alleviating T2D in mice.

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Supplementation of Bacillus sp. DU-106 Alleviates Antibiotic-Associated Diarrhea in Association with the Regulation of Intestinal Microbiota in Mice

Cited by 17 publications

References 49 publications

Protective effect of bioactive iridium nanozymes on high altitude-related hypoxia-induced kidney injury in mice

Protective effect of bioactive iridium nanozymes on high altitude-related hypoxia-induced kidney injury in mice

Regulatory Effect of Lactiplantibacillus plantarum 2-33 on Intestinal Microbiota of Mice With Antibiotic-Associated Diarrhea

Bacillus sp. DU-106 ameliorates type 2 diabetes by modulating gut microbiota in high-fat-fed and streptozotocin-induced mice

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