Supplementation of Fat Grafts With Adipose-Derived Regenerative Cells Improves Long-Term Graft Retention

Zhu, Min; Zhou, Zhengyu; Chen, Yan; Schreiber, Ronda E.; Ransom, John; Fraser, John K.; Hedrick, Marc H.; Pinkernell, Kai; Kuo, Hai-Chien

doi:10.1097/sap.0b013e31819ae05c

Cited by 281 publications

(206 citation statements)

References 33 publications

Supporting

Mentioning

189

Contrasting

Unclassified

Order By: Relevance

“…7,8 Recently, the addition of adipose-derived stem cells (ASCs) to whole fat grafts was proposed to support the formation of a new vasculature 9,10 and promote graft retention. 11 Fat tissue is a rich source of both endothelial progenitors 12,13 and multipotent mesenchymal stem cells (MSCs), which when expanded in short-term culture have been termed ASC. [14][15][16] ASC phenotypically resemble bone marrow (BM) MSCs 14 and share their multipotentiality, 15 but are far more prevalent in fat than BM-MSC are in bone marrow aspirates.…”

Section: Introductionmentioning

confidence: 99%

Regenerative Therapy and Cancer: In Vitro and In Vivo Studies of the Interaction Between Adipose-Derived Stem Cells and Breast Cancer Cells from Clinical Isolates

Zimmerlin

Donnenberg

Rubin

et al. 2011

Tissue Engineering Part A

213

143

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Regenerative Therapy and Cancer: In Vitro and In Vivo Studies of the Interaction Between Adipose-Derived Stem Cells and Breast Cancer Cells from Clinical Isolates

Zimmerlin

Donnenberg

Rubin

et al. 2011

Tissue Engineering Part A

213

143

View full text Add to dashboard Cite

“…Less common complications include hematoma, cellulitis responsive to antibiotics, fibrosis, oil cysts, and calcification. 19 Results are potentially good. After the procedure there were no signs of ulceration or discharge.…”

Section: Complicationsmentioning

confidence: 98%

An intervention study of autologous fat transfer for scar tissue rejuvenation in the setup

Parikh

Darshan

2018

Int Surg J

View full text Add to dashboard Cite

Background: There are lot of work going on AFT procedures. This procedure has been taken recent advances nowadays and very helpful in many conditions to rejuvenate and restoration of damaged tissues. The use of autologous fat for soft tissue augmentation seems attractive because of its relatively ubiquitous availability. Autologous fat transfer is a technique shown to be beneficial as a reconstructive procedure for patients with volume loss of tissue due to disease, trauma, and congenital defects.Methods: In the present study, 20 patients were taken as sample size. It is an intervention study. Whole procedure has been taken as day case which included local anaesthesia and patient received all the standard monitoring, medications, safety precautions and care that is provided for any patient undergoing any surgery, including antibiotics, pain medicine and other supportive care as needed. The Autologous fat was harvested by surgeon via syringe aspiration technique with a long atraumatic cannula. After processing, the material was injected immediately under designated skin grafts/scars using a small needle. Scar was cleansed with normal saline and dry dressing is applied for a day. Then patient was discharged on the same day.Results: Results obtained were efficacious with 60 to 80% improvement in all parameters in 15-20 days after the procedure.Conclusions: AFT procedure was found feasible, efficacious and accepted in patients with scar. It brings scar tissue rejuvenation up to great extent.

show abstract

“…Adipose-derived regenerative cells (ADRCs) are the nonbuoyant cellular fraction derived from the enzymatic digestion of adipose tissue. ADRCs contain several types of stem and regenerative cells, including ADSCs, vessel-forming cells such as endothelial and smooth muscle cells and their progenitors, and preadipocytes [30]. ADRCs can be easily isolated in large amounts from autologous adipose tissue and used without culturing or differentiation induction, which highlights the potential clinical application of these cells.…”

Section: Introductionmentioning

confidence: 99%

Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis in Rats

Nomura

Watanabe

Matsubara

et al. 2014

Clin Orthop Relat Res

View full text Add to dashboard Cite

Background Adipose-derived stem cells have recently shown differentiation potential in multiple mesenchymal lineages in vitro and in vivo. These cells can be easily isolated in large amounts from autologous adipose tissue and used without culturing or differentiation induction, which may make them relatively easy to use for clinical purposes; however, their use has not been tested in a distraction osteogenesis model. Question/purposes The question of this animal study in a rodent model of distraction osteogenesis was whether uncultured adipose-derived regenerative cells (ADRCs), which can easily be isolated in large amounts from autologous adipose tissue and contain several types of stem and regenerative cells, promote bone formation in distraction osteogenesis. We evaluated this using several tools: (1) radiographic analysis of bone density; (2) histological analysis of the callus that formed; (3) biomechanical testing; (4) DiI labeling (a method of membrane staining for postimplant celltracing); and (5) real-time polymerase chain reaction. Methods Sixty rats were randomly assigned to three groups. Physiological saline (control group), Type I collagen gel (collagen group), or a mixture of ADRC and Type I collagen gel (ADRC group) was injected into the distracted callus immediately after distraction termination. To a rat femur an external fixator was applied at a rate of 0.8 mm/day for 8 days.Results The bone density of the distracted callus in the ADRC group increased by 46% (p = 0.003, Cohen's d = 10.2, 95% confidence interval [CI] ± 0.180) compared with the control group at 6 weeks after injection. The fracture strength in the ADRC group increased by 66% (p = 0.006, Cohen's d = 1.32, 95% CI ± 0.180) compared with the control group at 6 weeks after injection. Real-time reverse transcription-polymerase chain reaction of the distracted callus from the ADRC group had higher levels of bone morphogenetic protein-2 (7.4 times higher), vascular endothelial growth factor A (6.8 times higher), and stromal cell-derived factor-1 (4.3 times higher). Cell labeling in the newly formed bone showed the ADRCs differentiated into osseous tissue at 3 weeks after injection. Conclusions The injection of ADRCs promoted bone formation in the distracted callus and this mechanism involves both osteogenic differentiation and secretion of humoral factors such as bone morphogenetic protein-2 or vascular endothelial growth factor A that promotes osteogenesis or angiogenesis.

show abstract

Supplementation of Fat Grafts With Adipose-Derived Regenerative Cells Improves Long-Term Graft Retention

Cited by 281 publications

References 33 publications

Regenerative Therapy and Cancer: In Vitro and In Vivo Studies of the Interaction Between Adipose-Derived Stem Cells and Breast Cancer Cells from Clinical Isolates

Regenerative Therapy and Cancer: In Vitro and In Vivo Studies of the Interaction Between Adipose-Derived Stem Cells and Breast Cancer Cells from Clinical Isolates

An intervention study of autologous fat transfer for scar tissue rejuvenation in the setup

Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis in Rats

Contact Info

Product

Resources

About