Supplementation of GelMA with Minimally Processed Tissue Promotes the Formation of Densely Packed Skeletal-Muscle-Like Tissues

Negrete, Jorge Alfonso Tavares; Pedroza-González, Sara Cristina; Frías-Sánchez, Ada I.; Salas-Ramírez, Miriam L.; Santiago-Miramontes, María  de los Ángeles de; Luna-Aguirre, Claudia Maribel; Álvarez, Mario Moisés; Santiago, Grissel Trujillo‐de

doi:10.1021/acsbiomaterials.2c01521

Cited by 3 publications

(2 citation statements)

References 80 publications

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“…GelMA maintained functional sequences of gelatin (Arg–Gly–Asp and the matrix metalloproteinase) and could form hydrogel with tunable physicochemical properties by photo-crosslinking [ 125 ]. The GelMA-combined dECM scaffold with different forms has shown extraordinary behaviors in regenerating various tissues [ 126–128 ]. For instance, Gao et al .…”

Section: Decm–natural Polymer Composite Materialsmentioning

confidence: 99%

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Xu,

Kankala,

Wang

et al. 2023

Regenerative Biomaterials

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Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, the clinical transformation of these scaffolds remained a big challenge because of the difficulty of simulating native organs/tissues' microenvironment. As a kind of natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based scaffolds have gained attention due to their unique biomimetic properties, providing a specific microenvironment suitable for promoting cell proliferation, migration, attachment, and regulating differentiation. The medical applications of dECM-based scaffolds have addressed critical challenges, including poor mechanical strength and insufficient stability. For promoting the reconstruction of damaged tissues or organs, different types of dECM-based composite platforms have been designed to mimic tissue microenvironment, including by integrating with natural polymer or/and syntenic polymer or adding bioactive factors. In this review, we summarized the research progress of dECM-based composite scaffolds in regenerative medicine, highlighting the critical challenges and future perspectives related to the medical application of these composite materials.

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Section: Decm–natural Polymer Composite Materialsmentioning

confidence: 99%

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Xu,

Kankala,

Wang

et al. 2023

Regenerative Biomaterials

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“…Introducing GA led to reduced porosity and smaller pore dimensions. Photo-cross-linking the vinyl double bond in GelMA added further stability to the hydrogel’s hydrophobic domains . GA-enriched hydrogels (GG and GGG) manifested significantly more hydrophobic regions compared to single-network GelMA hydrogel.…”

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confidence: 99%

A Hybrid Hydrogel with Intrinsic Immunomodulatory Functionality for Treating Multidrug-Resistant Pseudomonas aeruginosa Infected Diabetic Foot Ulcers

Qi,

Shi,

Zhang

et al. 2024

ACS Materials Lett.

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Contemporary options for multidrug-resistant bacteria infected diabetic foot ulcers (IDFUs) are predominantly nonspecific. These IDFU injuries often display prolonged inflammation and delayed tissue repair, mainly attributed to an overabundance of M1 macrophages in the hostile diabetic microenvironment. Although immunomodulatory hydrogels show promise for IDFU-focused care, a targeted, safe transition from M1 to M2 macrophages using simplified techniques remains a significant obstacle. Here, we introduce a hybrid hydrogel (GGG) with inherent immunomodulatory capabilities for IDFUs. GGG is composed of interpenetrating polymer networks featuring gallium-induced self-assembling glycyrrhizic acid and a photo-cross-linked gelatin methacryloyl matrix. Importantly, GGG effectively decreases the ratio of M1 to M2 macrophages in conditions infected with drug-resistant bacteria by disrupting iron metabolism and scavenging reactive oxygen species, which contributes to enhanced treatment results for diabetic wounds. To sum up, the immunomodulatory strategy we present provides a straightforward, safe, and highly effective therapeutic avenue for managing IDFUs.

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Continuous inline monitoring of glucose in an organ-on-chip using FreeStyle™ libre glucometers

Sánchez-Salazar,

Garza-Garza,

Crespo-López Oliver

et al. 2024

Front. Lab. Chip. Technol.

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Introduction: Organ-on-chips have become an effective platform for studying the physiology of tissues and organs and for evaluating the safety and efficacy of drugs. In these systems, the inline monitoring of key parameters of biological performance (i.e., glucose, oxygen, or lactic acid concentrations) provides valuable information regarding the cell/tissue physiological state. However, significant limitations still exist when attempting to obtain inline information in these systems, and the microsensing technology of on-chip measurement of key parameters is still limited by size, cost, and availability.Methods: Here, we demonstrate the use of a commercially available glucometer (FreestleTM Libre; Abbott), normally used for continuous determination of blood glucose levels, to provide continuous inline measurements of the glucose concentration in tumor-on-chips. Here, we employed a colorectal tumor-on-chip as a first demonstration model and measured the on-chip concentration of glucose continuously for extended culture times (2 weeks).Results and Discussion: We show that inline glucose readings are reproducible and enable the accurate determination of glucose consumption rates (GCRs) by a tumor cell culture. In turn, the GCR measurements provide valuable information regarding the changes in the metabolic activity of the on-chip cultures following inlet perturbations (i.e., delivery of pulses of glucose, culture media additives, and drugs). Inline continuous glucose sensors will be useful tools in organ-on-chip research and will greatly enable cancer research in tumor-on-chip systems.

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Supplementation of GelMA with Minimally Processed Tissue Promotes the Formation of Densely Packed Skeletal-Muscle-Like Tissues

Cited by 3 publications

References 80 publications

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

A Hybrid Hydrogel with Intrinsic Immunomodulatory Functionality for Treating Multidrug-Resistant Pseudomonas aeruginosa Infected Diabetic Foot Ulcers

Continuous inline monitoring of glucose in an organ-on-chip using FreeStyle™ libre glucometers

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