Supplementation of tris-based extender with plasma egg yolk of six avian species and camel skim milk for chilled preservation of dromedary camel semen

Panahi, Farnaz; Niasari-Naslaji, Amir; Seyedasgari, F.; Ararooti, T.; Razavi, K.; Moosavi-Movaheddi, Ali Akbar

doi:10.1016/j.anireprosci.2017.06.008

Cited by 10 publications

(3 citation statements)

References 47 publications

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“…The sperm characterization in camelids is not so much developed as in other mammalian species (Valverde et al, 2016;Yániz et al, 2016: Soler et al, 2017, although some works were developed on sperm characterization in camelids (Bravo et al, 1997;Bravo et al, 1999;Soler et al, 2014bSoler et al, , 2014cCarretero et al, 2015;Laruta-Limachi et al, 2016;Carretero et al, 2017;Panahi et al, 2017;Meza et al, 2018). The aim of the present work was to evaluate the effect of three different staining techniques on alpaca sperm morphometry analysed by CASA-Morph system.…”

Section: Introductionmentioning

confidence: 96%

Comparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system

Cucho¹,

yolvi²,

Caldeira³

et al. 2019

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Section: Introductionmentioning

confidence: 96%

Comparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system

Cucho¹,

yolvi²,

Caldeira³

et al. 2019

nbr

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“…The Steridyl contains irradiated sterile egg yolk while the Triladyl supplemented with in-lab prepared egg yolk. Interestingly, it has been reported that the source of plasma and egg yolk influence the ability of Tris-based extenders to maintain semen motility in chilled dromedary camel ejaculates [42]. Similarly, the egg yolk source has been shown to affect the post-thawing sperm motility and in vivo fertility in buffalo [23,43].…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Commercial Egg Yolk-Free and Egg Yolk-Supplemented Tris-Based Extenders for Dromedary Camel Semen Cryopreservation

Swelum

Saadeldin

Al-Mutary

et al. 2019

Animals

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Simple SummaryThis study compared the efficiency of commercial egg yolk-free (AndroMed, OPTIXcell) and egg yolk-supplemented (Triladyl, Steridyl) Tris-based extenders for semen cryopreservation in dromedary camels. The camel-specific extender SHOTOR was used for reference. SHOTOR, Triladyl, Steridyl, AndroMed, and OPTIXcell can all be used for camel semen cryopreservation; however, SHOTOR and Triladyl provide the best post-thawing sperm quality.AbstractThis study compared the efficiency of commercial egg yolk-free (AndroMed, OPTIXcell) and egg yolk-supplemented (Triladyl, Steridyl) Tris-based extenders for semen cryopreservation in seven adult dromedary camels. The camel-specific extender SHOTOR was used as control. The collected semen samples were evaluated and diluted with SHOTOR, Triladyl, Steridyl, AndroMed, or OPTIXcell. The diluted semen was gradually cooled and equilibrated for two hours before liquid nitrogen freezing. Semen was evaluated prior to freezing and after freeze-thawing cycles for motility, kinetics, vitality, abnormality, plasma membrane integrity, and DNA fragmentation using computer-assisted sperm analysis. In pre-freezing evaluation, progressive sperm motility was higher in SHOTOR-diluted samples (21.54 ± 1.83) than in samples diluted with Steridyl, OPTIXcell, or AndroMed (15.76 ± 1.80, 17.43 ± 1.10, and 13.27 ± 1.07, respectively). Moreover, Triladyl and SHOTOR resulted in significantly (p < 0.05) better sperm vitality and DNA integrity than all other diluents, but Triladyl resulted in a significantly (p < 0.05) better plasma membrane integrity (87.77 ± 0.31) than SHOTOR (85.48 ± 0.58). In the post-thawing evaluation, Triladyl led to significantly (p < 0.05) higher sperm motility (38.63 ± 0.81%; p < 0.05) when compared to SHOTOR, Steridyl or AndroMed (35.09 ± 1.341%, 34.4 ± 0.84%, and 31.99 ± 1.48%, respectively), with OPTIXcell being the least efficient (28.39 ± 0.86%). Progressive sperm motility was the highest when using Triladyl. Post-thawing curvilinear, straight line and average path sperm velocities were highest with Triladyl and lowest with AndroMed. Triladyl led to the highest linearity coefficient and straightness sperm coefficient, while SHOTOR to the highest DNA and plasma membrane integrity. OPTIXcell and AndroMed resulted in poor post-thawing sperm vitality, while Steridyl was less efficient than Triladyl. The highest rate of sperm abnormalities was recorded with OPTIXcell and the lowest with SHOTOR or Triladyl. In conclusion, SHOTOR, Triladyl, Steridyl, AndroMed, and OPTIXcell can all be used for camel semen cryopreservation; however, SHOTOR and Triladyl provided the best post-thawing sperm quality. Based on our findings, Triladyl is the best commercially available extender for dromedary camel semen cryopreservation to date.

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“…For example, α-lactoglobulin has been shown to be deleterious to the survival of equine sperm [ 12 ]. It has been reported that the source of egg yolk in Tris-based extenders affects semen motility in chilled dromedary camel ejaculates [ 13 ], and on post-thawing sperm motility and fertility in buffalo [ 14 ]. This could be attributed to the differences in fatty acid, phospholipids, cholesterol, and lipoprotein levels in egg yolk [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions

Nikitkina¹,

Musidray²,

Krutikova³

et al. 2020

Animals

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The fertilizing ability of stallion sperm after freezing is lower than in other species. The search for the optimal extender, combination of extenders, and the freezing protocol is relevant. The aim of this study was to compare lactose-chelate-citrate-yolk (LCCY) extender, usually used in Russia, and Steridyl® (Minitube) for freezing sperm of stallions. Steridyl is a concentrated extender medium for freezing ruminant semen. It already contains sterilized egg yolk. Semen was collected from nine stallions, aged from 7 to 12 years old. The total and progressive motility of sperm frozen in Steridyl was significantly higher than in semen frozen in LCCY. The number of spermatozoa with normal morphology in samples frozen in LCCY was 60.4 ± 1.72%, and with Steridyl, 72.4 ± 2.10% (p < 0.01). Semen frozen in Steridyl showed good stimulation of respiration by 2.4-DNP, which indicates that oxidative phosphorylation was retained after freezing–thawing. No differences among the extenders were seen with the DNA integrity of spermatozoa. Six out of ten (60%) mares were pregnant after artificial insemination (AI) by LCCY frozen semen, and 9/12 (75%) by Steridyl frozen semen. No differences among extenders were seen in pregnancy rate. In conclusion, Steridyl was proven to be a good diluent for freezing stallion semen, even though it was developed for ruminants.

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Supplementation of tris-based extender with plasma egg yolk of six avian species and camel skim milk for chilled preservation of dromedary camel semen

Cited by 10 publications

References 47 publications

Comparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system

Comparison of three different staining methods for the morphometric characterization of Alpaca (Vicugna pacos) sperm, using ISAS® CASA-Morph system

Efficiency of Commercial Egg Yolk-Free and Egg Yolk-Supplemented Tris-Based Extenders for Dromedary Camel Semen Cryopreservation

Efficiency of Tris-Based Extender Steridyl for Semen Cryopreservation in Stallions

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