Supplementation of watermelon peels as an enhancer of lipase and esterase production by<i>Yarrowia lipolytica</i>in solid-state fermentation and their potential use as biocatalysts in poly(ethylene terephthalate) (PET) depolymerization reactions

Sales, Julio Cesar Soares; Castro, Aline Machado de; Ribeiro, Bernardo Dias; Coelho, Maria Alice Zarur

doi:10.1080/10242422.2020.1782387

Cited by 23 publications

(10 citation statements)

References 59 publications

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“…As lipases microbianas podem ser utilizadas como aditivos em alimentos para modificar e realçar as propriedades organolépticas presentes, como também assumir a função de detergentes para hidrolisar gorduras, no tratamento de efluentes oleosos, além da presença nas industriais farmacêuticas, de cosméticos, agroquímicas e oleoquímicas. São considerados biocatalisadores versáteis que podem ser aplicados em diferentes reações químicas existentes: como hidrólise, esterificação, transesterificação e aminólise (MESSIAS et al 2011, RIBEIRO, et al 2011, MEHTA, BODH, GUPTA, 2017COSTA et al 2020, SALES et al 2020.…”

Section: Introductionunclassified

“…Segundo a Mordor Intelligence (2020), as lipases são responsáveis por 10% da participação do mercado mundial de enzimas e esse mercado tende a crescer 8,8% até 2025. Enzimas que apresentam a capacidade de hidrolisar apenas acilgliceróis de cadeia com menos de 10 carbonos são denominadas genericamente como esterases, diferindo das lipases por especificidade de substrato (BORNSCHEUER, 2002;AVHAD, MARCHETTI, 2019;SALES et al 2020).…”

Section: Introductionunclassified

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Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

França

Marinho

Cândido

et al. 2020

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RESUMO As esterases (E.C. 3.1.1.1) são enzimas pertencentes à classe das carboxil ester hidrolases, e atuam diretamente na clivagem e na formação de ligações ésteres, enquanto as lipases (EC 3.1.1.3) são hidrolases que catalisam a hidrólise de triacilgliceróis em ácidos graxos livres e glicerol. A utilização de enzimas nas indústrias permite o desenvolvimento de processos tecnológicos tão eficientes quanto os realizados pela natureza e sem impactos ambientais. Nesse contexto, o objetivo deste estudo foi identificar micro-organismos isoaldos da Caatinga com potencial promissor na produção das enzimas esterase e lipase. Amostras de fungos filamentosos (6 amostras) do gênero Aspergillus sp. denominados de SIS (10, 11, 12, 14, 92, 112) e 2 isolados de Penicillium sp. denominados de SIS (21, 23) foram cultivados em meios de cultura específico utilizando as temperaturas de 28°C e 35°C, durante 72 h, com acompanhamento diário. A presença do halo característico evidenciou a presença das enzimas testadas. Os melhores resultados obtidos indicaram que a produção de lipase e esterase com o Penicillium sp. (SIS 23), apresentando índice enzimático de 2,0cm à 35°C e á 28°C e 1,5 cm após 72 horas de cultivo destacando-se como um potencial produtor de esterase e lipase. Palavras-chave: detecção de enzimas microbianas, fungos filamentosos, enzimas hidrolíticas ABCTRACT Esterases (E.C. 3.1.1.1) are enzymes belong to the class of carboxyl ester hydrolases and act directly in cleavage and formation of ester bonds, while the lipases (EC 3.1.1.3) are hydrolases that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol). The use of enzymes in industries allows the development of technological processes as efficient as those carried out by nature and without environmental impacts. In this context, the objective this study was identify microorganisms isolated from Caatinga with promising potential in production of enzymes esterase and lipase. Samples of filamentous fungi (6 samples) Aspergillus sp. genus, called SIS (10, 11, 12, 14, 92, 112) and 2 isolates of Penicillium sp., called SIS (21, 23) were grown in specific culture means using temperatures of 28 °C and 35 °C during 72 h, with daily monitoring. The presence of the characteristic halo showed the presence of the tested enzymes. The best results obtained indicated the production of lipase and esterase by Penicillium sp. (SIS 23) with enzyme index of 2.0 cm at 35 °C and at 28 ° C and 1.5 cm after 72 hours cultivation standing out as potential producer of esterase and lipase.

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Section: Introductionunclassified

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

França

Marinho

Cândido

et al. 2020

BJD

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“…The authors also investigated its application, in short, medium, and long-chain esters synthesis. Additionally, aiming at polyethylene terephthalate (PET) degradation, Sales et al [86] tested the combined use of watermelon peels and soybean meal to induce esterase and lipase production. This enzyme pool led to a higher concentration of terephthalic acid during PET hydrolysis than the enzyme pool obtained with soybean meal alone, showing potential for other hydrolysis applications.…”

Section: Lipase Production By Yarrowia Lipolytica In Solid-state Ferm...mentioning

confidence: 99%

A Temporal Evolution Perspective of Lipase Production by Yarrowia lipolytica in Solid-State Fermentation

et al. 2022

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Lipases are enzymes that, in aqueous or non-aqueous media, act on water-insoluble substrates, mainly catalyzing reactions on carboxyl ester bonds, such as hydrolysis, aminolysis, and (trans)esterification. Yarrowia lipolytica is a non-conventional yeast known for secreting lipases and other bioproducts; therefore, it is of great interest in various industrial fields. The production of lipases can be carried on solid-state fermentation (SSF) that utilizes solid substrates in the absence, or near absence, of free water and presents minimal problems with microbial contamination due to the low water contents in the medium. Moreover, SSF offers high volumetric productivity, targets concentrated compounds, high substrate concentration tolerance, and has less wastewater generation. In this sense, the present work provides a temporal evolution perspective regarding the main aspects of lipase production in SSF by Y. lipolytica, focusing on the most relevant aspects and presenting the potential of such an approach.

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“…Bioengineering 2022, 9, 98 2 of 29 with energy generation process, whereas 40% is disposed of in landfills and 19% ends up in the oceans or on coastlines [7].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Biological Recycling of Polyethylene Terephthalate (PET) Plastic Wastes

2022

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Polyethylene terephthalate (PET) is one of the most commonly used polyester plastics worldwide but is extremely difficult to be hydrolyzed in a natural environment. PET plastic is an inexpensive, lightweight, and durable material, which can readily be molded into an assortment of products that are used in a broad range of applications. Most PET is used for single-use packaging materials, such as disposable consumer items and packaging. Although PET plastics are a valuable resource in many aspects, the proliferation of plastic products in the last several decades have resulted in a negative environmental footprint. The long-term risk of released PET waste in the environment poses a serious threat to ecosystems, food safety, and even human health in modern society. Recycling is one of the most important actions currently available to reduce these impacts. Current clean-up strategies have attempted to alleviate the adverse impacts of PET pollution but are unable to compete with the increasing quantities of PET waste exposed to the environment. In this review paper, current PET recycling methods to improve life cycle and waste management are discussed, which can be further implemented to reduce plastics pollution and its impacts on health and environment. Compared with conventional mechanical and chemical recycling processes, the biotechnological recycling of PET involves enzymatic degradation of the waste PET and the followed bioconversion of degraded PET monomers into value-added chemicals. This approach creates a circular PET economy by recycling waste PET or upcycling it into more valuable products with minimal environmental footprint.

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Supplementation of watermelon peels as an enhancer of lipase and esterase production byYarrowia lipolyticain solid-state fermentation and their potential use as biocatalysts in poly(ethylene terephthalate) (PET) depolymerization reactions

Cited by 23 publications

References 59 publications

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

A Temporal Evolution Perspective of Lipase Production by Yarrowia lipolytica in Solid-State Fermentation

Recent Advances in Biological Recycling of Polyethylene Terephthalate (PET) Plastic Wastes

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