Supplementation with Lactiplantibacillus plantarum IMC 510 Modifies Microbiota Composition and Prevents Body Weight Gain Induced by Cafeteria Diet in Rats

Abstract

Changes in functionality and composition of gut microbiota (GM) have been associated and may contribute to the development and maintenance of obesity and related diseases. The aim of our study was to investigate for the first time the impact of Lactiplantibacillus (L.) plantarum IMC 510 in a rat model of diet-induced obesity, specifically in the cafeteria (CAF) diet. This diet provides a strong motivation to voluntary overeat, due to the palatability and variety of selected energy-dense foods. The oral administration for 84 days of this probiotic strain, added to the CAF diet, decreased food intake and body weight gain. Accordingly, it ameliorated body mass index, liver and white adipose tissue weight, hepatic lipid accumulation, adipocyte size, serum parameters, including glycemia and low-density lipoprotein levels, in CAF fed rats, potentially through leptin control. In this scenario, L. plantarum IMC 510 showed also beneficial effects on GM, limiting the microbial imbalance established by long exposure to CAF diet and preserving the proportion of different bacterial taxa. Further research is necessary to better elucidate the relationship between GM and overweight and then the mechanism of action by which L. plantarum IMC 510 modifies weight. However, these promising results prompt a clear advantage of probiotic supplementation and identify a new potential probiotic as a novel and safe therapeutic approach in obesity prevention and management.

Probiotic Mechanisms Affecting Glucose Homeostasis: A Scoping Review

“…Oral intake of probiotic preparations of the strains Lactobacillus casei and Bifidobacterium bifidum (alone or both simultaneously) reduced the incidence of hyperglycaemia (increased blood glucose concentration) and dyslipidaemia (imbalance of blood lipid concentration) in rats with induced diabetes [ 79 ]. Similar antidiabetic effects in rats were obtained by administration of probiotic strains Lactobacillus plantarum TN627 [ 80 ], Bifidobacterium animalis 01 [ 81 ], Lactobacillus paracasei HII01 [ 82 ], 9 strains of Lactobacillus rhamnosus , Bifidobacterium adolescentis and Bifidobacterium bifidum [ 77 ], Lactobacillus rhamnosus BSL and R23 [ 83 ], heat-inactivated Streptococcus thermophilus [ 84 ], Lactiplantibacillus plantarum IMC 510 [ 85 ] and Lactobacillus fermentum RS-2 [ 86 ]. In the latter case, in addition to reduced glucose levels in fasting state, a low oxidative stress was observed [ 86 ].…”

Effects of probiotic Lactiplantibacillus plantarum IMC 510 supplementation on metabolic factors in otherwise healthy overweight and obese individuals

“…Each capsule contained 1.5 × 10 10 bacterial cells of the human origin strain Lact. plantarum IMC 510, isolated and characterized by Synbiotec S.r.l., and deposited at the Deutsche Sammlung von Mikroorganismen und Zelkulturen (DSMZ) collection with the identification number DSM 32548 (Coman et al, 2021;Coman & Cresci, 2014;Silvi et al, 2003). Compared with the probiotic capsules, the placebo ones contained just the same carrier material and were similar in size, shape and taste.…”

“…The potential probiotic strain Lactiplantibacillus plantarum IMC 510, tested in the present pilot clinical trial, was previously isolated from healthy human subjects, identified by 16S rRNA gene sequence analysis, characterized as probiotic strain and tested for safety (resistance to low pH, bile salts and pancreatic juice, ability to adhere to intestinal cells and colonize the mucosa, antipathogenic activity against gram-positive and gram-negative bacteria and yeasts, non-transmissible antibiotic resistance genes', absence of plasmids) by Synbiotec S.r.l. (Coman et al, 2021;Coman & Cresci, 2014;Silvi et al, 2003). Furthermore, Lact.…”

“…Furthermore, Lact. plantarum IMC 510 was evaluated in vivo in obese rats and it was able to decrease food intake, weight gain and, consequently, to induce relative beneficial effects, demonstrated by serological, biochemical and histological analyses, potentially through leptin control (Micioni Di Bonaventura et al, 2021). We hypothesized that daily consumption of one capsule of this probiotic strain could modulate the intestinal GM, thereby reducing body weight and fat, positively influencing several biomarkers of obesity-associated disorders including diabetes, hypertension and cardiovascular disease.…”

Exploratory Study for Probiotic Enrichment of a Sea Fennel (Crithmum maritimum L.) Preserve in Brine

“…Lactiplantibacillus plantarum IMC 509 is a further strain isolated by Synbiotec S.r.l. from the intestinal tract of elderly humans and is well characterized for its probiotic traits [ 15 , 17 , 18 , 19 ].…”