Abstract:Certain hormones play important roles in modulating mammalian reproductive behaviour. Daidzein is a well-known isoflavonic phytoestrogen that possesses oestrogenic activity. This study was conducted to probe the effects of daidzein supplementation in gestation diets on the reproductive performance in sows. A total of 120 multiparous sows (Landrace × Yorkshire) were randomly assigned to 2 groups (
n
= 60) and fed either a base diet (control) or one containing 200 mg/kg daidzein during ges… Show more
“…In the current study, we report three main findings: first, smaller sIUGR fetuses had lower placental SLC38A1 expression, contrasting with the upregulation 9 Disease Markers seen with miR-373-3p; second, introducing the miR-373-3p mimic or inhibitor to trophoblast cells led to SLC38A1 downregulation or upregulation, respectively; and third, we identified an interaction between miR-373-3p and the 3′-UTR of SLC38A1, experimentally verifying the regulation of the latter by the former. Other studies have also recognized the important contribution of SLC38A1 as an amino acid transporter in placental and fetal development [42][43][44][45][46]. Similarly, research has already described the relationship of SLC38A1 with miR-150-5p, miR-593-3p, and miR-4317 in specific pathological conditions [47][48][49].…”
The genetic pathogenesis of selective intrauterine growth restriction (sIUGR) remains elusive, with evidence suggesting an important role of epigenetic factors such as microRNAs. In this study, we explored the relevance of miR-373-3p to the occurrence of sIUGR. Hypoxia enhanced the levels of miR-373-3p and hypoxia-inducible factor (HIF)-1α, while HIF-1α knockdown not only boosted the migration and proliferation of HTR8 cells but also suppressed the hypoxia-induced upregulation of miR-373-3p and SLC38A1. By contrast, HIF-1α overexpression induced miR-373-3p downregulation and SLC38A1 upregulation, reducing cell growth and migration, which could be reversed by a miR-373-3p inhibitor. Importantly, the miR-373-3p inhibitor and mimic reproduced phenomena similar to those induced by HIF-1α downregulation and overexpression, respectively (including altered SLC38A1 expression, mTOR activation, cell growth, and migration). Mechanistically, the miRNA regulated cell behaviors and related mTOR signaling by targeting SLC38A1 expression through an interaction with the 3
′
-untranslated region of SLC38A1. The placental tissues of smaller sIUGR fetuses exhibited miR-373-3p and HIF-1α upregulation, SLC38A1 downregulation, and activated mTOR. Overall, miR-373-3p appears to restrict the growth and migration of HTR8 trophoblast cells by targeting SLC38A1, as observed in the placental tissues associated with smaller sIUGR fetuses, and it could have utility in the diagnosis and treatment of this disorder.
“…In the current study, we report three main findings: first, smaller sIUGR fetuses had lower placental SLC38A1 expression, contrasting with the upregulation 9 Disease Markers seen with miR-373-3p; second, introducing the miR-373-3p mimic or inhibitor to trophoblast cells led to SLC38A1 downregulation or upregulation, respectively; and third, we identified an interaction between miR-373-3p and the 3′-UTR of SLC38A1, experimentally verifying the regulation of the latter by the former. Other studies have also recognized the important contribution of SLC38A1 as an amino acid transporter in placental and fetal development [42][43][44][45][46]. Similarly, research has already described the relationship of SLC38A1 with miR-150-5p, miR-593-3p, and miR-4317 in specific pathological conditions [47][48][49].…”
The genetic pathogenesis of selective intrauterine growth restriction (sIUGR) remains elusive, with evidence suggesting an important role of epigenetic factors such as microRNAs. In this study, we explored the relevance of miR-373-3p to the occurrence of sIUGR. Hypoxia enhanced the levels of miR-373-3p and hypoxia-inducible factor (HIF)-1α, while HIF-1α knockdown not only boosted the migration and proliferation of HTR8 cells but also suppressed the hypoxia-induced upregulation of miR-373-3p and SLC38A1. By contrast, HIF-1α overexpression induced miR-373-3p downregulation and SLC38A1 upregulation, reducing cell growth and migration, which could be reversed by a miR-373-3p inhibitor. Importantly, the miR-373-3p inhibitor and mimic reproduced phenomena similar to those induced by HIF-1α downregulation and overexpression, respectively (including altered SLC38A1 expression, mTOR activation, cell growth, and migration). Mechanistically, the miRNA regulated cell behaviors and related mTOR signaling by targeting SLC38A1 expression through an interaction with the 3
′
-untranslated region of SLC38A1. The placental tissues of smaller sIUGR fetuses exhibited miR-373-3p and HIF-1α upregulation, SLC38A1 downregulation, and activated mTOR. Overall, miR-373-3p appears to restrict the growth and migration of HTR8 trophoblast cells by targeting SLC38A1, as observed in the placental tissues associated with smaller sIUGR fetuses, and it could have utility in the diagnosis and treatment of this disorder.
“…1 The basic structures of subclassified polyphenols ( Ahmadifar et al., 2021 ; Sobhani et al., 2021 ). ∗∗Polyphenols reported in sows ( Farmer et al., 2010 ; Norrby et al., 2011 ; Fan et al., 2015 ; Hu et al., 2015 ; Scott et al., 2015 ; Meng et al., 2018 ; Garcia-Contreras et al., 2019 ; Wang et al., 2019a ; Jiang et al., 2020 ; Li et al., 2021a , 2021b ); ∗polyphenols reported in swine except for sows ( Wu et al., 2004 ; Chen et al., 2018 ; Wan et al., 2018 ; Cai et al., 2020 ; Liao et al., 2020 ; Pomothy et al., 2020 ; Xu et al., 2020a , 2020b ; Long et al., 2021a ; Wang et al., 2021 ). Fig.…”
Section: The Structural Characteristics Of Polyphenolsmentioning
confidence: 99%
“…of piglets born and piglets born alive); Elevated serum antioxidant status of sows (↑ T-AOC [G 35 and G 85 ], SOD [G 35 and G 85 ], and GSH-Px [G 35 ]); Improved serum immunity of sows (↑ IgG [G 35 ]); Enhanced placental nutrient transport (↑ SNAT1 mRNA expression). Li et al. (2021b) Daidzein Dosage: 200 mg/kg Duration: G 0 –G 35 Improved reproductive performance of sows (↑ the no.…”
Section: The Structural Characteristics Of Polyphenolsmentioning
confidence: 99%
“…Recent studies have suggested that the dietary addition of polyphenols can effectively improve the antioxidant and immune functions of sows ( Fan et al., 2015 ; Hu et al., 2015 ; Meng et al., 2018 ; Wang et al., 2019a ; Jiang et al., 2020 ; Sun et al., 2020 ; Li et al., 2021b ; Long et al., 2021b ; Papatsiros et al., 2022 ). Flavonoids are the most abundant subgroup of polyphenols, and multiple flavonoids, including daidzein ( Li et al., 2021b ), glycitein ( Hu et al., 2015 ), and catechins ( Fan et al., 2015 ), have been demonstrated to promote the antioxidant and immune functions of sows. Daidzein is an isoflavonic phytoestrogen that is abundant in soybeans and other legumes ( Ren et al., 2001 ).…”
Section: The Latest Research Progress On Plant-derived Polyphenols In...mentioning
confidence: 99%
“…Daidzein is an isoflavonic phytoestrogen that is abundant in soybeans and other legumes ( Ren et al., 2001 ). Li et al. (2021b) demonstrated that the dietary addition of 200 mg/kg of daidzein in sow diets throughout the gestation period elevated the total antioxidant capacity (T-AOC) and immunoglobulin G (IgG) concentration, the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum of sows on d 35 of pregnancy, as well as the T-AOC and the activities of SOD in the serum of sows on d 85 of pregnancy.…”
Section: The Latest Research Progress On Plant-derived Polyphenols In...mentioning
A natural product is a compound or substance originating from a living organism and found in nature. Daidzein belongs to the class of bioflavonoids, which are plant‐derived compounds with various biological activities. Predominantly exists in soybeans and several legumes in either glycoside or aglycone forms. Its primary chemical constituents include metabolites like equol and trihydroxy isoflavone, generated through the influence of intestinal bacteria. Daidzein has exhibited pharmacological impacts on different ailments, including cardiovascular disease, cancer, diabetes, skin disorders, osteoporosis, and neurodegenerative disorders. Daidzein's mechanisms of action involve interactions with estrogen receptors, as well as its antioxidant and anti‐inflammatory properties, along with its ability to regulate apoptosis and the cell cycle. In efforts to enhance its solubility, stability, bioavailability, and targeting, daidzein has been innovatively formulated into novel dosage forms, including nanoparticles, liposomes, microemulsions, and nanosuspensions. As a promising nutraceutical, daidzein presents multiple health benefits and holds potential for various clinical applications. Additional investigation is required to comprehend the molecular mechanisms of this phenomenon and assess its safety. The purpose of this review is to provide a short description of the therapeutic properties, chemical composition, traditional use, toxicology profile, new insights on the dosage form, and future prospects of daidzein.
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