Supported Lipid Membranes at the Au‐Buffer Interface by Solvent Exchange: The Effect of Initial Solvent Concentration

Betlem, Kaï; Cordoyiannis, George; Losada-Pérez, Patricia

doi:10.1002/pssa.201900837

Cited by 6 publications

(5 citation statements)

References 32 publications

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“…Note that the Δ f and Δ D shifts due to step 1 are caused by changes in the viscosity and density of the bulk solution, and not protein adsorption itself. It is thus important to compare Δ f and Δ D shifts only when the initial and final values were recorded in the same bulk solution. , For this reason, the results of protein adsorption in the water–ethanol mixtures (relative to the water–ethanol baseline without protein, before and after water–ethanol mixture washing) were evaluated along with the final adsorbate properties after aqueous buffer washing (relative to the initial baseline values in aqueous buffer).…”

Section: Resultsmentioning

confidence: 99%

Unraveling How Ethanol-Induced Conformational Changes Affect BSA Protein Adsorption onto Silica Surfaces

et al. 2020

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Protein adsorption at solid−liquid interfaces is highly relevant to a wide range of applications such as biosensors, drug delivery, and pharmaceuticals. Understanding how protein conformation in bulk solution impacts adsorption behavior is fundamentally important and could also lead to the development of improved protein-based coatings. To date, relevant studies have been conducted in aqueous solutions, while it remains largely unknown how organic solvents and more specifically solvent-induced conformational changes might influence protein adsorption. Herein, using the quartz crystal microbalance-dissipation (QCM-D) and localized surface plasmon resonance (LSPR) techniques, we systematically investigated the real-time adsorption behavior of bovine serum albumin (BSA) protein onto silica surfaces in different water−ethanol mixtures ranging from 0 to 60% (v/v) ethanol. The results showed that there was greater protein adsorption at higher ethanol fractions in the 10−30% range, while more complex adsorption profiles were observed in the 40−60% range. The combination of QCM-D and LSPR measurements led us to further identify specific cases in water−ethanol mixtures where washing steps caused densification of the adsorbed protein layer as opposed to typical desorption of weakly adsorbed molecules in aqueous conditions. We discuss mechanistic factors that drive these overall adsorption trends by taking into account how ethanol fraction affects BSA conformation in bulk solution. Together, our findings demonstrate that BSA proteins can adsorb onto silica surfaces across a wide range of water−ethanol mixture conditions, while specific adsorption profiles depended on the ethanol fraction in a manner closely linked to solution-phase conformational properties.

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Section: Resultsmentioning

confidence: 99%

Unraveling How Ethanol-Induced Conformational Changes Affect BSA Protein Adsorption onto Silica Surfaces

et al. 2020

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“…The phase transition of pure DMPC when incubated at low temperatures displays a double-peak behavior. The presence of the two peaks has been previously observed in the literature from calorimetric and QCM-D measurements, and it is a direct consequence of the extrusion process and related to structural transitions in the vesicle itself [ 36 , 37 ].…”

Section: Resultsmentioning

confidence: 94%

Asymmetric Lipid Transfer between Zwitterionic Vesicles by Nanoviscosity Measurements

et al. 2021

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The interest in nano-sized lipid vesicles in nano-biotechnology relies on their use as mimics for endosomes, exosomes, and nanocarriers for drug delivery. The interactions between nanoscale size lipid vesicles and cell membranes involve spontaneous interbilayer lipid transfer by several mechanisms, such as monomer transfer or hemifusion. Experimental approaches toward monitoring lipid transfer between nanoscale-sized vesicles typically consist of transfer assays by fluorescence microscopy requiring the use of labels or calorimetric measurements, which in turn require a large amount of sample. Here, the capability of a label-free surface-sensitive method, quartz crystal microbalance with dissipation monitoring (QCM-D), was used to monitor lipid transfer kinetics at minimal concentrations and to elucidate how lipid physicochemical properties influence the nature of the transfer mechanism and dictate its dynamics. By studying time-dependent phase transitions obtained from nanoviscosity measurements, the transfer rates (unidirectional or bidirectional) between two vesicle populations consisting of lipids with the same head group and differing alkyl chain length can be estimated. Lipid transfer is asymmetric and unidirectional from shorter-chain lipid donor vesicles to longer-chain lipid acceptor vesicles. The transfer is dramatically reduced when the vesicle populations are incubated at temperatures below the melting of one of the vesicle populations.

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“…Apart from vesicle adsorption and rupture described earlier, the systematic use of QCM-D will greatly contribute to the understanding of emerging alternative approaches to form supported lipid membranes spanning from bicelle adsorption, [69] to solventassisted lipid bilayer formation, [70,71] and inkjet printing. [72] George Cordoyiannis obtained his Ph.D. in physics at the National Technical University of Athens, Greece.…”

Section: Discussionmentioning

confidence: 99%

QCM‐D Study of the Formation of Solid‐Supported Artificial Lipid Membranes: State‐of‐the‐Art, Recent Advances, and Perspectives

Bar

Villanueva

Neupane

et al. 2023

Physica Status Solidi (a)

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Solid‐supported lipid bilayers (SLBs) are excellent platforms for studying the biophysical properties of cell membranes. Among the existing approaches used to form SLBs, vesicle fusion and rupture onto solid supports are most commonly employed owing to their straightforward procedure. The current understanding of the mechanisms behind this approach has greatly benefited from the use of surface‐sensitive techniques, especially quartz crystal microbalance with dissipation monitoring (QCM‐D) in combination with other analytical techniques, such as atomic force microscopy (AFM) or localized surface plasmon resonance (LSPR). Herein, an overview of the pathways of vesicle adsorption and rupture under various experimental conditions is provided. Examples including recent findings of how the variation of the properties of lipid vesicles (size, charge), aqueous buffer (pH, ionic strength, osmotic pressure), and solid support (surface energy) affect the pathway mechanism of adsorption and rupture are provided. Recent reports on poorly understood properties such as surface roughness and topography are provided, together with the need for further studies relevant to biomimetic and sensing purposes.

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Supported Lipid Membranes at the Au‐Buffer Interface by Solvent Exchange: The Effect of Initial Solvent Concentration

Cited by 6 publications

References 32 publications

Unraveling How Ethanol-Induced Conformational Changes Affect BSA Protein Adsorption onto Silica Surfaces

Unraveling How Ethanol-Induced Conformational Changes Affect BSA Protein Adsorption onto Silica Surfaces

Asymmetric Lipid Transfer between Zwitterionic Vesicles by Nanoviscosity Measurements

QCM‐D Study of the Formation of Solid‐Supported Artificial Lipid Membranes: State‐of‐the‐Art, Recent Advances, and Perspectives

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