Supportive Role of Probiotic Strains in Protecting Rats from Ovariectomy-Induced Cortical Bone Loss

Montazeri‐Najafabady, Nima; Ghasemi, Younes; Dabbaghmanesh, Mohammad Hossein; Talezadeh, Pedram; Koohpeyma, Farhad; Gholami, Ahmad

doi:10.1007/s12602-018-9443-6

Cited by 72 publications

(87 citation statements)

References 36 publications

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“…(121) In addition to altering the gut microbiome and reducing intestinal inflammation, probiotics are known to promote intestinal barrier function and benefit bone health. (50,(53)(54)(55)(56)(57)63,67,68,71,73,75,76,(122)(123)(124)(125)(126)(127)(128)(129)(130)(131)(132) Consistent with these studies, we found that treatment with LR as well as LGG uniquely altered the microbiota composition, which was significantly different from mice treated with GC alone. We hypothesize that each of these compositions have a different balance of healthy versus disease-promoting bacteria, which could be playing a role in its differential effects on the intestinal barrier and furthermore bone health.…”

Section: Discussionsupporting

confidence: 85%

Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

Schepper

Collins

Rios‐Arce

et al. 2019

Journal of Bone and Mineral Research

133

119

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Glucocorticoids (GCs) are potent immune‐modulating drugs with significant side effects, including glucocorticoid‐induced osteoporosis (GIO). GCs directly induce osteoblast and osteocyte apoptosis but also alter intestinal microbiota composition. Although the gut microbiota is known to contribute to the regulation of bone density, its role in GIO has never been examined. To test this, male C57/Bl6J mice were treated for 8 weeks with GC (prednisolone, GC‐Tx) in the presence or absence of broad‐spectrum antibiotic treatment (ABX) to deplete the microbiota. Long‐term ABX prevented GC‐Tx‐induced trabecular bone loss, showing the requirement of gut microbiota for GIO. Treatment of GC‐Tx mice with a probiotic (Lactobacillus reuteri [LR]) prevented trabecular bone loss. Microbiota analyses indicated that GC‐Tx changed the abundance of Verrucomicobiales and Bacteriodales phyla and random forest analyses indicated significant differences in abundance of Porphyromonadaceae and Clostridiales operational taxonomic units (OTUs) between groups. Furthermore, transplantation of GC‐Tx mouse fecal material into recipient naïve, untreated WT mice caused bone loss, supporting a functional role for microbiota in GIO. We also report that GC caused intestinal barrier breaks, as evidenced by increased serum endotoxin level (2.4‐fold), that were prevented by LR and ABX treatments. Enhancement of barrier function with a mucus supplement prevented both GC‐Tx–induced barrier leakage and trabecular GIO. In bone, treatment with ABX, LR or a mucus supplement reduced GC‐Tx–induced osteoblast and osteocyte apoptosis. GC‐Tx suppression of Wnt10b in bone was restored by the LR and high‐molecular‐weight polymer (MDY) treatments as well as microbiota depletion. Finally, we identified that bone‐specific Wnt10b overexpression prevented GIO. Taken together, our data highlight the previously unappreciated involvement of the gut microbiota and intestinal barrier function in trabecular GIO pathogenesis (including Wnt10b suppression and osteoblast and osteocyte apoptosis) and identify the gut as a novel therapeutic target for preventing GIO. © 2019 American Society for Bone and Mineral Research.

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Section: Discussionsupporting

confidence: 85%

Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

Schepper

Collins

Rios‐Arce

et al. 2019

Journal of Bone and Mineral Research

133

119

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“…For example, whether vitamin D supplementation should be considered in African countries to reduce vaginal L. iners or rise L. crispatus , as was tried recently in a pregnant cohort (Jefferson et al, 2019). Gut probiotics such as L. casei , B. longum have been reported to increase vitamin D in ovariectomy-induced mice model of osteoporosis (Montazeri-Najafabady et al, 2018), and our results suggest that there might be counterparts in the female reproductive tract after getting married and pregnant.…”

Section: Discussionsupporting

confidence: 52%

The vagino-cervical microbiome as a woman’s life history

Jie

Liang

et al. 2019

Preprint

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The vagina contains at least a billion microbial cells, which are dominated byLactobacilli. Here we perform metagenomic shotgun sequencing on cervical samples from 1148 women. Factors such as pregnancy, delivery histories and breast-feeding were all more important than menstrual cycle in shaping the microbiome. Bifidobacterium breve was seen with older age at sexual debut;Lactobacillus crispatus negatively correlated with pregnancy history; potential markers for lack of menstrual regularity, heavy flow, dysmenorrhea, contraceptives were also identified. Other features such as mood fluctuations and facial speckles could potentially be deduced from the vagino-cervical microbiome. Gut and oral microbiome, plasma vitamins, metals, amino acids and hormones showed associations with the vagino-cervical microbiome. Our results offer an unprecedented glimpse into the microbiota of the female reproductive tract and call for international collaborations to better understand its long-term health impact. Highlights:Shotgun sequencing of 1148 vagino-cervical samples reveal subtypes; 1Other omics such as moods and skin features could be predicted by the vagino-cervical 2 bacteria; 3Factors such as delivery mode and breast-feeding associate with the vagino-cervical 4 microbiome; 5With dwindled Lactobacilli, postmenopausal samples are relatively enriched for viruses. 6 7 3 4 vulvovaginal candidiasis (Bradford and Ravel, 2017). The over 90% of human 30 sequences in female reproductive tract samples, in contrast to 1% in feces (Li et al., 31 2018; Methé et al., 2012; Wang and Jia, 2016), has made metagenomic shotgun 32 sequencing more expensive. Most studies of the vaginal microbiota used 16S rRNA 33 gene amplicon sequencing, which lacked a view of the overall microorganism 34 communities including bacteria, archaea, viruses and fungi (Byrd et al., 2018), as well 35 as the functional capacity encoded. Besides infection, studies on current sexual activity 36 and the menstrual cycle occurred naturally to the vaginal microbiota field (Gajer et al., 37 2012; Ravel et al., 2010). However, lasting impacts from other potentially important 38 factors such as sexual debut, pregnancy and breast-feeding have not been looked at in 39 a reasonably large cohort. 40 41 As a sizable reservoir of microbes instead of a transient entity, the female reproductive 42 tract microbiota might also reflect conditions in other body sites. It is however not clear 43 whether other omics in circulation or in the intestine cross-talks with the vagino-cervical 44 microbiome. Intersecting with hormones, metabolic and immune functions, we find it 45intriguing to explore the potential link of the vagino-cervical microbiome to the brain and 46 the face. 47 5 data, immune indices, physical fitness test, facial skin imaging, as well as female life 53 history questionnaire, lifestyle questionnaire, and psychological questionnaire (Figure 1). 54Our work pinpoints other metadata or omics that can predict or be predicted from the 55 microbiota in the female reproductive tr...

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“…The probiotics were isolated from traditional fermented dairy products, produced in Iran according to Montazeri et al (16). Brie y, 10 g of each dairy samples was homogenized, serially diluted and cultured on TSA medium.…”

Section: Bacterial Isolation and Formulationmentioning

confidence: 99%

“…It is supposed that their bene cial effect is related to many internal mechanisms including the skeletal system beyond control of blood glucose (15). In a preceding study, we reported that some types of probiotics could improve the bone formation, reduced bone resorption, and changes in the microstructure of the femur in ovariectomized-induced bone loss rat model (16,17). Dar et al disclosed that administration of lactobacillus acidophilus for 12 weeks could attenuate the bone loss and improve the mechanical bone strength in streptozotocin-induced diabetic rats with no impacts on glucose concentration (18).…”

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confidence: 97%

Probiotics Ameliorate Pioglitazone-associated Bone Loss in Diabetic Rats

Gholami

Dabbaghmanesh

Ghasemi

et al. 2020

Preprint

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Background: Pioglitazone as a PPAR-g agonist are used for management of type 2 diabetes mellitus. Nevertheless, evidence showed that the therapeutic modulation of PPARg activity using Pioglitazone may be linked with bone mass reduction and fracture risk in type 2 diabetes mellitus patients. The objective of the current research was to inspect the preventive role of some types of probiotic strains including (Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Bifidiobacterum longum and Bacillus coagulans) against pioglitazone-induced bone loss.Methods: Streptozotocin (60 mg/kg) was administered for diabetes induction. Diabetic rats were fed orally with pioglitazone (300 mg/kg) and probiotics (1×109 CFU/ml/day) alone and in combination of both for 4 weeks. Dual energy X-ray absorptiometry (DEXA) were used to asses BMD, BMC and area of the femur, spine and tibia at the experiment termination. Serum glucose, serum calcium, alkaline phosphatase, phosphorus, BUN, Creatinine, and urine calcium were also analyzed. Results: Administration of pioglitazone and probiotics alone and in combination significantly improved elevated blood glucose. Pioglitazone treatment significantly increased urinary calcium and BUN, and decreased ALP and creatinine. Co-treatment of probiotics with pioglitazone significantly decreased urinary calcium, creatinine and ALP. Pioglitazone showed detrimental effects on femur-BMD whereas treatment with probiotics remarkably ameliorated these effects. Among the tested probiotics Bifidiobacterum longum displayed the best protective effects on pioglitazone-induced bone loss in diabetic rats.Conclusion:This study suggests probiotic supplementation in diabetic patients on pioglitazone regime could be considering as a good strategy to ameliorate bone loss induced by pioglitazone.

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Supportive Role of Probiotic Strains in Protecting Rats from Ovariectomy-Induced Cortical Bone Loss

Cited by 72 publications

References 36 publications

Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

Involvement of the Gut Microbiota and Barrier Function in Glucocorticoid-Induced Osteoporosis

The vagino-cervical microbiome as a woman’s life history

Probiotics Ameliorate Pioglitazone-associated Bone Loss in Diabetic Rats

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