Suppression of Aromatase Activity in Polycystic Ovary Syndrome

Magoffin, Denis A.; Agarwal, Sanjay; Jakimiuk, Artur

doi:10.1007/978-1-4613-8483-0_14

Cited by 4 publications

(5 citation statements)

References 45 publications

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“…The pathogenesis of PCOS in women is an androgen disorder that can increase the activity of the steroidogenesis pathway in ovarian follicles, which is achieved through theca and granulosa cells. In theca cells, it increases the expression of the enzyme 17βhydroxysteroid dehydrogenase (17β HSD), which causes an increase in testosterone levels in granulosa cells (Magoffin, 2006). Increased adrenal activity causes insulin phosphorylation, which causes insulin resistance, affecting approximately 50% -70% of women (Pate & Sirmans, 2014).…”

Section: Introductionmentioning

confidence: 99%

In vivo Evaluation of Extracted and Fraction of Moringa oleifera leaves against Testosterone-Induced PCOS Model in Rattus Norvegicus

Khairi,

Nuraini Harmastuti,

Gunawan Pamudji Widodo

2023

JFIKI

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Background: Polycystic ovary syndrome (PCOS) is an endocrine disorder. Parameters characteristic of the disorder include weight gain, insulin resistance and malondialdehyde (MDA). The traditional medicine used is Moringa oleifera. Objectives: The aim was to determine the effect of weight loss and blood glucose levels, MDA levels and histopathological features of the ovarian follicles in the rat model PCOS-insulin resistance induced by testosterone. Methods: Extraction process, followed by fractionation using n-hexane, ethyl acetate and water, identification of compound content using TLC, and rats were grouped into 7 groups (n=5), namely normal group, negative group, positive group, extract, fraction water, ethyl acetate fraction and n-hexane fraction by looking at the characteristic parameters and ovarian histopathology. Data analysis using ANOVA and Kruskal-wallis. Results: The yield of the extract was 30.4%, the water fraction was 85.59%, the ethyl acetate fraction was 6.64% and the n-hexane fraction was 4.05%. Positive for flavonoids, tannins, alkaloids in the ethyl acetate fraction, the water and extract fractions were positive for tannins, the n-hexane fraction was positive for steroids. The modeling sample group obtained extract body weight 195.40 g, water fraction 195.80 g, ethyl acetate fraction 194.00 g, n-hexane fraction 196.00 g, blood glucose level extract 83.00 mg/dL, water fraction 84.27 mg/dL, ethyl acetate fraction 80.00 mg/dL, n-hexane fraction 122.85 mg/dL, MDA extract content 2.704 nmol/mL, water fraction 3.547 nmol/mL, 1.685 nmol/mL, 5.308 nmol /mL and can improve ovarian histopathology. Conclusion: The most effective value is the ethyl acetate fraction because it has the highest decrease in PCOS characteristics.

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Section: Introductionmentioning

confidence: 99%

In vivo Evaluation of Extracted and Fraction of Moringa oleifera leaves against Testosterone-Induced PCOS Model in Rattus Norvegicus

Khairi,

Nuraini Harmastuti,

Gunawan Pamudji Widodo

2023

JFIKI

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“…The pathophysiology and underlying aetiology of PCOS are still not well understood [ 5 , 6 ]. Among the postulated etiopathologies of PCOS are the aberrations in steroidogenesis [ 3 , 7 ], folliculogenesis [ 8 ], and aromatase enzyme action [ 9 , 10 ]. Genomic and molecular investigations have shown that increased androgen synthesis is an inherent steroidogenic abnormality in PCOS, particularly involving steroidogenic enzymes found in ovarian theca cells [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

“…Another dysregulated enzyme in PCOS is the aromatase enzyme encoded by cytochrome P450 family 19 subfamily A member 1 Cyp19a1 [ 9 ]. Aromatase catalyzes the rate-determining step during the conversion of androgens into oestrogens [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Kelulut Honey Improves Folliculogenesis, Steroidogenic, and Aromatase Enzyme Profiles and Ovarian Histomorphology in Letrozole-Induced Polycystic Ovary Syndrome Rats

Kamal

Ibrahim²,

Ugusman³

et al. 2022

Nutrients

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Polycystic ovary syndrome (PCOS) has been linked to aberrant folliculogenesis and abnormalities in the aromatase enzyme (Cyp19a1) and the steroidogenic enzyme, 17-alpha-hydroxylase (Cyp17a1) expression. It has been demonstrated that Kelulut honey (KH) improves both female and male reproductive system anomalies in animal studies. Here, we examined the effects of isolated and combined KH, metformin, and clomiphene in improving folliculogenesis, aromatase, and steroidogenic enzyme profiles and ovarian histomorphology in letrozole-induced PCOS rats. Letrozole (1 mg/kg/day) was administered to female Sprague–Dawley (SD) rats for 21 days to induce PCOS. PCOS rats were subsequently divided into six experimental groups: untreated, treatment with metformin (500 mg/kg/day), clomiphene (2 mg/kg/day), KH (1 g/kg/day), combined KH (1 g/kg/day) and metformin (500 mg/kg/day), and combined KH (1 g/kg/day) and clomiphene (2 mg/kg/day). All treatments were given orally for 35 days. We found that KH was comparable with clomiphene and metformin in improving the expression of Cyp17a1 and Cyp19a1, apart from enhancing folliculogenesis both histologically and through the expression of folliculogenesis-related genes. Besides, the combination of KH with clomiphene was the most effective treatment in improving the ovarian histomorphology of PCOS rats. The effectiveness of KH in restoring altered folliculogenesis, steroidogenic, and aromatase enzyme profiles in PCOS warrants a future clinical trial to validate its therapeutic effect clinically.

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“…To date, the etiopathogenesis of PCOS is still not well defined [ 7 , 8 ]. However, the suggested etiopathology of PCOS includes abnormalities in steroidogenesis [ 9 , 10 ], follicular arrest [ 11 ], deficiency in aromatase enzymes [ 12 ], and insulin-resistant hyperinsulinism [ 13 ]. Additionally, recent studies have investigated the role of sex steroid receptors in women with PCOS [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model

Kamal

Ibrahim

Ugusman

et al. 2022

IJMS

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Reproductive and metabolic anomalies in polycystic ovary syndrome (PCOS) have been associated with the dysregulation of sex steroid receptors. Kelulut honey (KH) has been shown to be beneficial in PCOS-induced rats by regulating folliculogenesis and the oestrus cycle. However, no study has been conducted to evaluate KH’s effect on sex steroid receptors in PCOS. Therefore, the current study examined the effects of KH, metformin, or clomiphene alone and in combination on the mRNA expression and protein distribution of androgen receptor (AR), oestrogen receptor α (ERα), oestrogen receptor β (ERβ), and progesterone receptor (PR) in PCOS-induced rats. The study used female Sprague-Dawley rats, which were treated orally with 1 mg/kg/day of letrozole for 21 days to develop PCOS. PCOS-induced rats were then divided and treated orally for 35 days with KH, metformin, clomiphene, KH + metformin, KH+ clomiphene and distilled water. In this study, we observed aberrant AR, ERα, ERβ and PR expression in PCOS-induced rats compared with the normal control rats. The effects of KH treatment were comparable with clomiphene and metformin in normalizing the expression of AR, ERα, and ERβ mRNA. However, KH, clomiphene and metformin did not affect PR mRNA expression and protein distribution. Hence, this study confirms the aberrant expression of sex steroid receptors in PCOS and demonstrates that KH treatment could normalise the sex steroid receptors profile. The findings provide a basis for future clinical trials to utilize KH as a regulator of sex steroid receptors in patients with PCOS.

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Suppression of Aromatase Activity in Polycystic Ovary Syndrome

Cited by 4 publications

References 45 publications

In vivo Evaluation of Extracted and Fraction of Moringa oleifera leaves against Testosterone-Induced PCOS Model in Rattus Norvegicus

In vivo Evaluation of Extracted and Fraction of Moringa oleifera leaves against Testosterone-Induced PCOS Model in Rattus Norvegicus

Kelulut Honey Improves Folliculogenesis, Steroidogenic, and Aromatase Enzyme Profiles and Ovarian Histomorphology in Letrozole-Induced Polycystic Ovary Syndrome Rats

Kelulut Honey Regulates Sex Steroid Receptors in a Polycystic Ovary Syndrome Rat Model

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