Suppression of liver receptor homolog-1 by microRNA-451 represses the proliferation of osteosarcoma cells

Li, Zhiyong; Wu, Shihao; Lv, Shouzheng; Wang, Huili; Wang, Yong; Guo, Qiang

doi:10.1016/j.bbrc.2015.04.013

Cited by 23 publications

(16 citation statements)

References 39 publications

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“…To date, the specific miRs that target and regulate LRH-1 have not been well recognized and investigated. A recent study elucidated that miR-451 directly targets and suppresses LRH-1 expression in osteosarcoma cells, thereby inhibiting cancer cell proliferation (56). In the present study, miR-381 targeted and regulated LRH-1 expression in HCC cells.…”

Section: Discussionsupporting

confidence: 55%

MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma

et al. 2015

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MicroRNAs (miRs) have emerged as prospective tools for human cancer therapy, including hepatocellular carcinoma (HCC) therapy. Previous studies have suggested that miR-381 functions as oncogenic or tumor-suppressive miRs in other cancer types. However, the role of miR-381 in HCC remains unknown. The present study investigated the expression and functional role of miR-381 in HCC. miR-381 expression was significantly decreased in HCC tissues and cell lines. miR-381 overexpression significantly inhibited HCC cell proliferation and colony formation, induced G0/G1 cell cycle arrest and suppressed cell invasion. Conversely, suppression of miR-381 showed the opposite effect in HCC cells. Bioinformatics analysis and dual-luciferase reporter assay results showed that miR-381 directly targeted the 3'-untranslated region of liver receptor homolog-1 (LRH-1), and quantitative polymerase chain reaction and western blot analysis results showed that miR-381 negatively modulated LRH-1 expression. Data elucidated that miR-381 directly regulated HCC cell growth and invasion, as well as the Wnt signaling pathways, by targeting LRH-1. Clinical tissue detection data revealed an inverse correlation between miR-381 and LRH-1 expression in HCC tissues, further indicating the functional significance of miR-381-LRH-1 in regulating HCC tumorigenesis. The present study indicates that miR-381 may be a novel tumor suppressor that blocks HCC growth and invasion by targeting LRH-1. The results present novel insights into understanding the molecular mechanism underlying HCC tumorigenesis and provide a future direction to the development of therapeutic interventions for HCC.

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Section: Discussionsupporting

confidence: 55%

MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma

et al. 2015

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“…19 LRH1 expression is also increased in pancreatic and osteosarcoma tumors compared to normal adjacent tissues. 13,20 Notably, high LRH1 expression was obviously correlated with poorer differentiation, advanced pTNM stage, ADC subtype, and, most importantly, lymphatic metastasis. Consistently, LRH1 gene polymorphisms significantly contribute to the possibility of distant metastasis or regional lymph node metastasis in gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

“…LRH1 expression was upregulated in mRNA or protein in tumor tissues compared to normal lung tissues. Elevated‐level gene amplification or overexpression of LRH1 has been found in various types of malignant carcinomas . qPCR assay showed that LRH1 mRNA expression is overexpressed in human gastric tumor tissues compared to self‐paired normal controls .…”

Section: Discussionmentioning

confidence: 99%

“…qPCR assay showed that LRH1 mRNA expression is overexpressed in human gastric tumor tissues compared to self‐paired normal controls . LRH1 expression is also increased in pancreatic and osteosarcoma tumors compared to normal adjacent tissues …”

Section: Discussionmentioning

confidence: 99%

“…Elevated-level gene amplification or overexpression of LRH1 has been found in various types of malignant carcinomas. 13,19,20 qPCR assay showed that LRH1 mRNA expression is overexpressed in human gastric tumor tissues compared to self-paired normal controls. 19 LRH1 expression is also increased in pancreatic and osteosarcoma tumors compared to normal adjacent tissues.…”

Section: Discussionmentioning

confidence: 99%

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LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non‐small cell lung cancer

et al. 2018

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BackgroundLRH1, which promotes the malignant transformation of carcinoma, has recently been documented in several types of malignancies. However, LRH1 has not been assessed as a potential clinical biomarker in any cancer.MethodsLRH1 expression was tested in fresh‐frozen tissue samples with quantitative real‐time PCR and Western blot analysis. Surgically resected tumor tissues were collected from 156 non‐small cell lung cancer (NSCLC) patients: 75 with adenocarcinoma and 81 with squamous cell carcinoma. Subsequently, the immunohistochemical expression of LRH1 was examined, and its clinical significance was evaluated.ResultsLRH1 overexpression was observed in NSCLC carcinoma tissues compared to adjacent normal lung tissues. LRH1 expression was correlated with poorer differentiation (P = 0.023), pathological tumor classification (P < 0.001), advanced pathological tumor node metastasis stage (P = 0.017), adenocarcinoma subtype (P = 0.031), and positive lymph node metastasis (P < 0.001). Multivariate analysis demonstrated that LRH1 expression status was an independent prognostic factor for overall (hazard ratio 1.372, 95% confidence interval 1.225–1.617; P = 0.003) and disease‐free survival (hazard ratio 1.497, 95% confidence interval 1.059–2.115; P = 0.011) in patients who suffered from resectable NSCLC.ConclusionThe results of our study indicate that LRH1 predicts NSCLC progression, metastasis, and a dismal prognosis, emphasizing its promising role as a novel target in NSCLC therapies.

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A comprehensive review on miR‐451: A promising cancer biomarker with therapeutic potential

Khordadmehr

Jigari-Asl

Ezzati

et al. 2019

Journal Cellular Physiology

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MicroRNAs (miRNAs) are proposed as a family of short noncoding molecules able to manage and control the expression of the gene targets at the posttranscriptional level. They contribute in several fundamental physiological mechanisms as well as a verity of human and animal diseases such as cancer progression. Among these tiny RNAs, miR-451 placed on chromosome 17 at 17q11.2 presents an essential role in many biological processes in health condition and also in pathogenesis of different diseases. Besides, it has been recently considered as a valuable biomarker for cancer detection, prognosis and treatment. Therefore, this review will provide the critical functions of miR-451 on biological mechanisms including cell cycle and proliferation, cell survival and apoptosis, differentiation and development as well as disease initiation and progression such as tumor formation, migration, invasion, and metastasis.

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Suppression of liver receptor homolog-1 by microRNA-451 represses the proliferation of osteosarcoma cells

Cited by 23 publications

References 39 publications

MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma

MicroRNA-381 suppresses cell growth and invasion by targeting the liver receptor homolog-1 in hepatocellular carcinoma

LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non‐small cell lung cancer

A comprehensive review on miR‐451: A promising cancer biomarker with therapeutic potential

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