2010
DOI: 10.1016/j.freeradbiomed.2010.02.002
|View full text |Cite
|
Sign up to set email alerts
|

Suppression of mutagenesis by 8-hydroxy-2′-deoxyguanosine 5′-triphosphate (7,8-dihydro-8-oxo-2′-deoxyguanosine 5′-triphosphate) by human MTH1, MTH2, and NUDT5

Abstract: To assess the functions of the three human MutT-type enzymes, MTH1, MTH2, and NUDT5, mutation induction by an oxidized form of dGTP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP, 7,8-dihydro-8-oxo-2'-deoxyguanosine 5'-triphosphate), was examined using human 293T cells treated with their specific siRNAs. Shuttle plasmid DNA containing the supF gene was first transfected into the cells, and then 8-OH-dGTP was introduced by means of osmotic pressure. Escherichia coli cells were transformed with the DNAs… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
30
0

Year Published

2010
2010
2024
2024

Publication Types

Select...
10

Relationship

1
9

Authors

Journals

citations
Cited by 53 publications
(31 citation statements)
references
References 45 publications
1
30
0
Order By: Relevance
“…Both the supF plasmid DNA and dG O TP were introduced into cells in which the expression of each protein was knocked-down. The A:T➔C:G substitution mutations induced by dG O TP were higher in the knockdown cells than in control cells [54]. The increase in the induced mutation was more evident in the triple knockdown cells.…”
Section: Introductionmentioning
confidence: 99%
“…Both the supF plasmid DNA and dG O TP were introduced into cells in which the expression of each protein was knocked-down. The A:T➔C:G substitution mutations induced by dG O TP were higher in the knockdown cells than in control cells [54]. The increase in the induced mutation was more evident in the triple knockdown cells.…”
Section: Introductionmentioning
confidence: 99%
“…Thus, in tumor cells, reducing the capacity to eliminate oxidized deoxyribonucleotides is likely to prove a novel and promising chemotherapeutic strategy, particularly in combination with drugs that induce oxidative stress. In a similar vein, assessing the levels and activity of MTH2 [160] and NUDT5, an enzyme that hydrolyzes 8-oxo-dGDP and 8-hydroxy-dADP [161], in cancer cells and determining their roles in inhibiting oxidative stress-induced DDR can provide additional therapeutic targets in the nucleotide pool sanitization pathways.…”
Section: Conclusion and Future Perspectivesmentioning
confidence: 99%
“…MTH1-a member of the family of hydrolases that eliminate oxidized precursors from the dNTP pool (Ishibashi et al, 2003)-degrades both 8-oxodGTP and 8-oxoGTP and prevents 8-oxoG accumulation in DNA and RNA (Sakumi et al, 1993;Hayakawa et al, 1999). MTH1 inactivation is associated with a mutator phenotype in mouse cells (Hori et al, 2010) and increased cancer incidence in mice (Tsuzuki et al, 2001). Recent findings link MTH1 deficiency to neurodegeneration.…”
Section: Introductionmentioning
confidence: 99%