2018
DOI: 10.1159/000487872
|View full text |Cite
|
Sign up to set email alerts
|

Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK

Abstract: Background/Aims: Reversion-inducing cysteine-rich protein with kazal motifs (RECK) is a novel tumor suppressor gene that is critical for regulating tumor cell invasion and metastasis. The expression of RECK is dramatically down-regulated in human cancers. Harmine, a tricyclic compound from Peganum harmala, has been shown to have potential anti-cancer activity. Methods: Cell proliferation assay (CCK-8 cell viability assay), cell cycle analysis (detection by flow cytometry), apoptosis staining assay (TUNEL stain… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
11
0

Year Published

2018
2018
2025
2025

Publication Types

Select...
10

Relationship

0
10

Authors

Journals

citations
Cited by 24 publications
(12 citation statements)
references
References 31 publications
1
11
0
Order By: Relevance
“…Cell proliferation was measured with an CCK-8 assay according to the manufacturer's instructions as described before [21]. Stably transfected U87 and U251 cells were seeded at a density of 10 3 /well into 96-well culture plates for 24, 48, 72, 96 and 120h.…”
Section: Cck-8 Assaymentioning
confidence: 99%
“…Cell proliferation was measured with an CCK-8 assay according to the manufacturer's instructions as described before [21]. Stably transfected U87 and U251 cells were seeded at a density of 10 3 /well into 96-well culture plates for 24, 48, 72, 96 and 120h.…”
Section: Cck-8 Assaymentioning
confidence: 99%
“…More than 1.5 million people are diagnosed with LC every year (1,2). Lung adenocarcinoma (LUAD) is the most common histological subtype of LC (3,4). The 5-year survival rate for LUAD patients is less than 15%, as most are diagnosed at advanced stages (5).…”
Section: Introductionmentioning
confidence: 99%
“…The tumor progression of orthotopic xenograft mouse models were verified using H&E staining in previous studies. 31,32 The H&E staining results of mouse lung tumors suggested that miR-30a or miR-30b inhibitors abrogated the tumor growth-inhibiting effect by the LDR pretreatments, thereby confirming the central role of PAI-1 in the radiation response of tumors (Figure 6F). Finally, by analyzing the orthotopically xenografted tumor, we showed that the HDR-mediated increase in the phosphorylation of ERK1/2 and Akt, and the levels of PAI-1 and EMT markers were significantly suppressed by LDR pretreatment (Figures 6G-6K).…”
Section: Systemic Delivery Of Mir-30a and Mir-30b Increased Radiosensitivity In Orthotopic Mouse Xenograft Modelmentioning
confidence: 63%