Suppression of Spontaneous Dermatitis in NC/Nga Murine Model by PG102 Isolated from Actinidia arguta

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease, which requires safe and effective pharmacological therapy. We previously found that two preparations from Actinidia arguta, PG102T, and PG102E, could modulate Th1/Th2 pathways and suppress IgE biosynthesis. This study was performed to assess the therapeutic effects of PG102T and PG102E on the development of dermatitis in NC/Nga mice, characterized by the spontaneous onset of AD along with an elevated level of IgE under conventional conditions. PG102T or PG102E administration significantly reduced dermatitis severity as well as scratching tendency in conventional mice. The suppression of dermatitis by PG102 was accompanied by a decrease in the plasma level of IgE, IgG1, and IL-4 and also by an increase in that of IgG2a and IL-12. The splenic level of IL-4, IL-5, and IL-10 was downregulated, whereas that of IFN-gamma and IL-12 was increased. The number of eosinophils and the expression of eotaxin and thymus and activation-regulated chemokine were decreased by PG102T or PG102E. Histological findings also indicated that the thickening of epidermis/dermis and the dermal infiltration of inflammatory cells including mast cells were greatly inhibited. These data suggest that PG102 may be effective therapeutic agents for the treatment of AD.

Dietary Supplementation with Lactic Acid Bacteria Attenuates the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice in a Strain-Dependent Manner

“…However, KW3110 also decreased both IgG1 and IgG2a levels in the plasma. These results are different from recent observations that CpG-oligodeoxynucleotides [31] and preparations from Actinidia arguta [32], which decreased IgE and IgG1 but conversely increased IgG2a. It is known that IgE and IgG1 are commonly induced by IL-4 but IgG2a is differently regulated [33, 34].…”

Suppressive Effect of CheongGiGeoYangTang on Allergic Inflammation of RBL-2H3 Mast Cells and OVA/alum-sensitized Mice

“…淸肌祛痒湯의 RBL-2H3 비만세포와 OVA/alum 감작 생쥐의 알레르기 염증 반응 억제 효과 11 , Balb/c, NC/Nga 마우스, Hairless 랫드와 같은 실 험동물을 이용하여 피부자극 16) , 식이유도 17) , 환경유도 18) , 오발부민(OVA, Ovalumin)감작 19) 등의 (Fig. 3-4).…”

Administration of Antibiotics during Infancy Promoted the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice

“…The total clinical score for dermatitis severity was defined as the sum of the individual scores graded as 0 (none), 1 (mild), 2 (moderate), and 3 (severe) for each of five signs and symptoms (itching, erythema/hemorrhage, edema, excoriation/erosion, and scaling/dryness). 22) Scratching behavior was observed for 10 min per mouse to evaluate scratching frequency. Observers blinded to the experimental groups performed these observations.…”