Suppression of v-Src Transformation by Andrographolide via Degradation of the v-Src Protein and Attenuation of the Erk Signaling Pathway

Liang, Fong Pin; Chao, Lin; Kuo, Cheng Deng; Chao, Hsueh-Ping; Fu, Shu Ling

doi:10.1074/jbc.m705877200

Cited by 46 publications

(42 citation statements)

References 47 publications

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“…Anti-inflammatory (Batkhuu et al 2002;Liu et al 2007;Parichatikanond et al 2010); Anti-parasitic (Misra et al 1992); Hepatoprotective (Chander et al 1995;Kapil et al 1993 (Yoopan et al 2007;; Anti Herpes (Wiart et al 2005); Vasorelaxant and Antihypertensive (Zhang and Tan 1999); Antioxidant and hepatoprotective (Akowuah et al 2009); Antithrombotic (Thisoda et al 2006); Cytotoxic (Tan et al 2005); Anti retroviral (Reddy et al 2005 Table 2 continued Chemical constituents: ent labdane diterpenoids Activity reported in literature Anticancer Shi et al (2008;Cheung et al 2005;Sheeja and Kuttan 2007b;Han et al 2006;Ji et al 2007;Manikam and Stanslas 2009;Zhou et al 2006;Liang et al 2008;…”

Section: Pharmacologymentioning

confidence: 96%

A bitter plant with a sweet future? A comprehensive review of an oriental medicinal plant: Andrographis paniculata

2011

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Andrographis paniculata (Burm.f) Nees is one of the most popular and important medicinal plant of the Orient, and South East Asia. It finds mention in various forms in Indian, Chinese, Malay, Thai, Unani, and Japanese systems of medicine. The plant exhibits anti-cancer, anti-inflammatory, anti-diabetic, antihypertensive, anti-venom, cholestatic, hepatoprotective, anti-thrombotic, anti-retroviral, anti-microbial, anti-pyretic, anti-malarial, anti-oxidant, immunomodulatory, and cardioprotective effects. The major active principles contributing to biological activity are diterpene lactones, but flavonoids, xanthones and caffeic acid derivatives also contribute to anti-oxidant, anti-proliferative, anti-atherosclerotic, and anti-malarial effects. As a result of its wide spectrum of pharmacological activity, almost impeccable safety profile, being a widely cultivated medicinal plant, we have collected and compiled various facets of this plant. Extensive datamining of the phytochemistry and pharmacology of Andrographis paniculata revealed more than 50 diterpene lactones, 30 flavonoids, 8 quinic acid derivatives, and 4 xanthones. This review contains information on around 80 isolated compounds, out of which more than half of the compounds have no reported pharmacological activity. Though there are some good reviews available on Andrographis paniculata, the authors of the earlier reviews focused on one or two aspects of the plant and none have attempted to integrate the available information on this plant. This provided us the much needed impetus, warranting a full-fledged and complete review on Andrographis paniculata, one of the most popular and important Oriental medicinal plant.

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Section: Pharmacologymentioning

confidence: 96%

A bitter plant with a sweet future? A comprehensive review of an oriental medicinal plant: Andrographis paniculata

2011

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“…3 In many cancer cell types, AP was also capable of inducing G 0 /G 1 cell-cycle arrest, 4,5 promoting tumor necrosis factor-related apoptosisinducing ligand (TRAIL; an important member of extrinsic apoptosis)-mediated apoptosis, 6 activating p53 via enhanced phosphorylation, and inhibiting nuclear factor-kappa B (NF-6B)-associated transcriptional and angiogenic factors. [7][8][9] There is good possibility that AP would be the next important naturally derived anticancer agent.…”

Section: Introductionmentioning

confidence: 99%

Poor oral bioavailability of a promising anticancer agent andrographolide is due to extensive metabolism and efflux by P‐glycoprotein

Wang

Tang

et al. 2011

Journal of Pharmaceutical Sciences

123

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“…In the previous studies, the expression of vimentin may have been regulated by Src via STAT3 activation [80,81]. However, in LA29-induced transformation of RK3E cells, the STAT3 pathway was not required for transformation [34]. By comparison, AKT pathway activation may increase vimentin's stability by phosphorylating vimentin and inhibiting caspase-induced degradation [82].…”

Section: Discussionmentioning

confidence: 95%

“…Temperaturesensitive v-Src-transformed RK3E cells have been previously reported to be used in monitoring regulation of Src activity [34]. Briefly, a temperature-sensitive v-Src mutant LA29 was Fig.…”

Section: Comparative Analysis Of Proteome Methylation In V-src-transfmentioning

confidence: 99%

“…RK3E-tva-RCAN (Control), RK3E-tva-LA29 (LA29), RK3E-pcDNA3, and RK3E-Src531 cell clones were generated as described previously [34]. Briefly, RK3E cells expressing avian viral receptor (tva) were infected with the avian retrovirus carrying a temperature-sensitive v-Src mutant LA29 [35,36] to generate the temperature-sensitive, v-Srctransformed RK3E cells.…”

Section: Cells and Cell Culturementioning

confidence: 99%

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Proteomics analysis of in vitro protein methylation during Src‐induced transformation

Chiou¹,

Fu²,

Lin³

et al. 2012

Electrophoresis

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Src, a nonreceptor tyrosine kinase, was the first oncogene identified from an oncogenic virus. Mechanistic studies of Src-induced transformations aid in understanding the pathologic processes underlying tumorigenesis and may provide new strategies for cancer therapy. Although several pathways and protein modifications are reportedly involved in Src-induced transformation, the detailed mechanisms of their regulation remain unclear. Protein methylation is an important PTM that is widely involved in cellular physiology. In this study, we determined if protein methylation was involved in Src activation and which methylated proteins were associated with this activity. Using in vitro methylation and 2-DE analysis of viral Src (v-Src)-transformed rat kidney epithelial cells (RK3E), several known and novel methylated proteins were identified based on their changes in methylation signal intensity upon transformation. Among these, elongation factor 2 (EF-2), heterogeneous nuclear ribonucleoprotein K (hnRNP K), and β-tubulin protein expressions remained unchanged, indicating that their altered methylation levels were due to Src activation. In addition, the altered expression of β-actin, vimentin, and protein phosphatase 2, catalytic subunit (PPP2C) as well as protein phosphatase 2, catalytic subunit methylation were also confirmed in RK3E cells transformed with a human oncogenic Src mutant (Src531), supporting their association with Src-induced transformation in human cancer. Together, we showed putative involvement of protein methylation in Src activation and our identification of methylated proteins provides important targets for extensively studying Src-induced transformations.

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Suppression of v-Src Transformation by Andrographolide via Degradation of the v-Src Protein and Attenuation of the Erk Signaling Pathway

Cited by 46 publications

References 47 publications

A bitter plant with a sweet future? A comprehensive review of an oriental medicinal plant: Andrographis paniculata

A bitter plant with a sweet future? A comprehensive review of an oriental medicinal plant: Andrographis paniculata

Poor oral bioavailability of a promising anticancer agent andrographolide is due to extensive metabolism and efflux by P‐glycoprotein

Proteomics analysis of in vitro protein methylation during Src‐induced transformation

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