Suppressive effect of nobiletin and epicatechin gallate on fructose uptake in human intestinal epithelial Caco-2 cells

Satsu, Hideo; Awara, Sohei; Unno, Toshihiro; Shimizu, Makoto

doi:10.1080/09168451.2017.1387515

Cited by 17 publications

(24 citation statements)

References 29 publications

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“…Furthermore, authors demonstrated that epicatechin gallate suppressed fructose permeation in Caco-2 cells, suggesting that this compound suppressed the transepithelial transport of fructose across epithelial cell monolayers, in addition to its effect on fructose uptake. Lastly, authors reported that similar effects on fructose uptake and permeation were observed with nobiletin, another phytochemical tested in this study [107].…”

Section: Plant Extracts Inhibitors Of Fructose Transporterssupporting

confidence: 82%

“…Likewise, Satsu et al demonstrated that epicatechin gallate inhibited fructose uptake in Caco-2 cells. Interestingly, this reduction in fructose uptake was not related to changes in the affinity (K m ) of GLUT5 for fructose, but with a decrease in the maximal velocity (V max ) [107]. Furthermore, authors demonstrated that epicatechin gallate suppressed fructose permeation in Caco-2 cells, suggesting that this compound suppressed the transepithelial transport of fructose across epithelial cell monolayers, in addition to its effect on fructose uptake.…”

Section: Plant Extracts Inhibitors Of Fructose Transportersmentioning

confidence: 98%

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Intestinal Fructose and Glucose Metabolism in Health and Disease

et al. 2019

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The worldwide epidemics of obesity and diabetes have been linked to increased sugar consumption in humans. Here, we review fructose and glucose metabolism, as well as potential molecular mechanisms by which excessive sugar consumption is associated to metabolic diseases and insulin resistance in humans. To this end, we focus on understanding molecular and cellular mechanisms of fructose and glucose transport and sensing in the intestine, the intracellular signaling effects of dietary sugar metabolism, and its impact on glucose homeostasis in health and disease. Finally, the peripheral and central effects of dietary sugars on the gut-brain axis will be reviewed.

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Section: Plant Extracts Inhibitors Of Fructose Transporterssupporting

confidence: 82%

Section: Plant Extracts Inhibitors Of Fructose Transportersmentioning

confidence: 98%

Intestinal Fructose and Glucose Metabolism in Health and Disease

et al. 2019

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“…5,20 In addition, there is no report of EGCG inhibiting PEPT1 activity, whereas (-)-epicatechin gallate, another gallate catechin, not inhibiting the uptake transport of glycyl-sarcosine, a typical substrate of PEPT1, in Caco-2 cells. 21 This suggests that catechins may display only a weak inhibitory potential on PEPT activity. OATPs could also be candidate influx transporters, because EGCG and (-)-epicatechin gallate have been shown to inhibit OATP1A2, OATP1B1, and OATP2B1 activities in vitro.…”

Section: Discussionmentioning

confidence: 99%

Impact of Green Tea Catechin Ingestion on the Pharmacokinetics of Lisinopril in Healthy Volunteers

Misaka

Ono

Uchida

et al. 2020

Clinical Translational Sci

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Lisinopril, a highly hydrophilic long‐acting angiotensin‐converting enzyme inhibitor, is frequently prescribed for the treatment of hypertension and congestive heart failure. Green tea consumption may reduce the risk of cardiovascular outcomes and total mortality, whereas green tea or its catechin components has been reported to decrease plasma concentrations of a hydrophilic β blocker, nadolol, in humans. The aim of this study was to evaluate possible effects of green tea extract (GTE) on the lisinopril pharmacokinetics. In an open‐label, randomized, single‐center, 2‐phase crossover study, 10 healthy subjects ingested 200 mL of an aqueous solution of GTE containing ~ 300 mg of (–)‐epigallocatechin gallate, a major catechin component in green tea, or water (control) when receiving 10 mg of lisinopril after overnight fasting. The geometric mean ratio (GTE/control) for maximum plasma concentration and the area under the plasma concentration‐time curve of lisinopril were 0.289 (90% confidence interval (CI) 0.226–0.352) and 0.337 (90% CI 0.269–0.405), respectively. In contrast, there were no significant differences in time to reach maximum lisinopril concentration (6 hours in both phases) and renal clearance of lisinopril (57.7 mL/minute in control vs. 56.9 mL/minute in GTE). These results suggest that the extent of intestinal absorption of lisinopril was significantly impaired in the presence of GTE, whereas it had no major effect on the absorption rate and renal excretion of lisinopril. Concomitant use of lisinopril and green tea may decrease oral exposure to lisinopril, and therefore result in reduced therapeutic efficacy.

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“…Caco-2 cell line is a human colorectal adenocarcinoma line with no mucus present, used in LGS studies as a model of the intestinal epithelial barrier. As a result, the fructose uptake is reduced to the levels below 60% of the control [57]. An in vivo study on rats fed with fructose and dietary polyphenol-chrysinshowed alterations in GLUT-5 expression leading to its lower activity [55].…”

Section: Fructose As a More Affecting Factor Than Glucosementioning

confidence: 99%

Dietary Carbohydrates and Lipids in the Pathogenesis of Leaky Gut Syndrome: An Overview

Binienda

Twardowska

Makaro

et al. 2020

IJMS

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This review summarizes the recent knowledge on the effects of dietary carbohydrates and lipids on the pathophysiology of leaky gut syndrome (LGS). Alterations in intestinal barrier permeability may lead to serious gastrointestinal (GI) disorders. LGS is caused by intestinal hyperpermeability due to changes in the expression levels and functioning of tight junctions. The influence of dietary habits on intestinal physiology is clearly visible in incidence rates of intestinal diseases in industrial and developing countries. Diseases which are linked to intestinal hyperpermeability tend to localize to Westernized countries, where a diet rich in fats and refined carbohydrates predominates. Several studies suggest that fructose is one of the key carbohydrates involved in the regulation of the intestinal permeability and its overuse may cause harmful effects, such as tight junction protein dysfunction. On the other hand, short chain fatty acids (mainly butyrate) at appropriate concentrations may lead to the reduction of intestinal permeability, which is beneficial in LGS. However, long chain fatty acids, including n-3 and n-6 polyunsaturated fatty acids have unclear properties. Some of those behave as components untightening and tightening the intestinal membrane.

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Suppressive effect of nobiletin and epicatechin gallate on fructose uptake in human intestinal epithelial Caco-2 cells

Cited by 17 publications

References 29 publications

Intestinal Fructose and Glucose Metabolism in Health and Disease

Intestinal Fructose and Glucose Metabolism in Health and Disease

Impact of Green Tea Catechin Ingestion on the Pharmacokinetics of Lisinopril in Healthy Volunteers

Dietary Carbohydrates and Lipids in the Pathogenesis of Leaky Gut Syndrome: An Overview

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