Suppressive effect of poly-γ-glutamate on SOS response of Salmonella typhimurium induced by chemical mutagens

Sato, Machiko; Kanie, Kyoko; Soda, Kenji; Yokoigawa, Kumio

doi:10.1263/jbb.105.690

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…The suppressive effect of c-PGA on the SOS response of Salmonella typhimurium induced by several direct and indirect mutagens has been studied and evaluated (Sato et al, 2008). The sodium salt of c-PGA was used with a range of molecular masses: 50, 2000, 4000, 6000 and 8000 kDa.…”

Section: Medical Applicationsmentioning

confidence: 99%

Poly-γ-glutamic acid: production, properties and applications

et al. 2015

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Section: Medical Applicationsmentioning

confidence: 99%

Poly-γ-glutamic acid: production, properties and applications

et al. 2015

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“…Previously, we reported that PGA suppressed the decrease in leavening ability during prolonged fermentation time, probably because PGA adsorbed the inhibitory metabolites that accumulated in the dough (Yokoigawa and others ). We also reported that PGA showed high antimutagenic activity by adsorbing chemical mutagens (Sato and others ). Here, we found that PGA precipitated beyond its saturated density well adsorbed Stx.…”

Section: Discussionmentioning

confidence: 77%

Adsorption of Shiga Toxin to Poly‐γ‐Glutamate Precipitated

Goto

Tsuji

Kanemaru

et al. 2016

Journal of Food Science

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We screened foods containing indigestible ingredients in the ability to adsorb Shiga toxin (Stx). When 5 mg of foods and dietary fibers such as dry vegetables and inulin were mixed and incubated with 0.5 mL of Stx solution (100 ng/mL) containing 0.5% bovine serum albumin, both Stx1 and Stx2 seemed to be adsorbed by only a fermented food, natto (a traditional Japanese food prepared from steamed soybeans by the biological action of Bacillus subtilis). We purified the Stx-adsorbing substance from natto by extraction with H O, acid treatment, Proteinase K treatment, and an ion exchange chromatography. The purified substance showed an average molecular mass of about 600 kDa. We identified it as poly-γ-glutamate (PGA) by amino acid analysis of its hydrolysate and peptide analysis after its treatment with Proteinase K. Purified PGA (MW: molecular weight = about 600 kDa) was considered to adsorb both Stx1 and Stx2 when we separated adsorbed and unadsorbed Stxs (MW = about 72 kDa) by an ultrafiltration method with a centrifugal filter unit (MWCO: molecular weight cut-off = 100 K). However, PGA with the ability to adsorb Stx was an insoluble form precipitated in the filter unit during centrifugation. PGA precipitated beyond the saturated density was also confirmed to well adsorb both Stx1 and Stx2 by an equilibrated dialysis method. To the best of our knowledge, this is the 1st report on food-adsorbing Stx.

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